结合

Clau (5, 10, 20〓) was bound to rat plasma a greater extent than clau, while in rabbit plasma the reverse was the case. By Scatchard plots using bovine serum albumin, clau had a higher equilibrium constant and the greater numbers of binding sites than its antipode.

在与大鼠血浆蛋白的结合中，clau的结合率平均为28.37±0.31（n=3，三个剂量5，10，20μM），clau的结合率平均为38.05±2.61（n=3），clau结合率低于clau；而在与兔血浆蛋白的结合中，clau的结合率平均为47.21±4.91（n=3，三个剂量），clau的结合率平均为41.43±5.21（n=3），clau的结合率略高于clau；在与牛血清白蛋白的结合中，clau的结合率也高于clau，立体选择方向与兔血浆蛋白结合相同，而与大鼠血浆蛋白结合相反，提示存在种属差异；在与系列浓度的牛血清白蛋白结合中，通过Scatchard作图法求得蛋白结合点数和平衡常数；在clau，N和K分别为90.02±35.76和5.326±1.196（n=4）；在clau，N和K分别为59.92±20.39和4.203±1.250（n=4），两者的比值分别为1.50和1.27；进一步说明clau对映体的蛋白结合存在立体选择性差异。

I think there was some coalescence结合联合 to..

我认为两党应该进行一些结合，但是我不知道。

Peripheral plasma membrane proteins extracted from the spermatozoa from caput,corpus and cauda epididymis as well as vas deferens were probed with four lectins—Arachis hypogaea agglutinin、Canavalia ensiformis agglutinin、Dolichos biflorus agglutinin、Triticum vulgaris agglutinin.The results indicated that the 104 and 95kD AHA binding glycoproteins,and the 117、114、104、87、76、70kD ConA binding glycoproteins were synthesized and modified in the testis;the 120 and 65kD ConA binding glycoproteins,and the 54kD DBA binding glycoprotein were modified or lost during epididymal maturation;the 109kD ConA binding glycoprotein,the 74、70、45kD DBA binding glycoproteins,and the 57kD TVA binding glycoprotein were modified during epididymal maturation.

结果表明：与花生凝集素结合的104kD和95kD糖蛋白，与伴刀豆凝集素结合的117、114、104、87、76、70kD糖蛋白在睾丸中已完成了蛋白合成及修饰过程；与伴刀豆凝集素结合的120kD和65kD糖蛋白，与双花扁豆凝集素结合的54kD糖蛋白在精子附睾成熟过程中被修饰成其它种类的蛋白或被丢失；与伴刀豆凝集素结合的109kD糖蛋白，与双花扁豆凝集素结合的74、70、45kD糖蛋白，以及与麦胚凝集素结合的57kD糖蛋白是在附睾中完成其修饰过程的。

In inequality in the equivalent transformation process requires a lot of mathematical ideas, discuss issues such as classification, the overall for-yuan,数形结合, transformation and so on as appropriate to the infiltration of these thinking, to improve the mathematical abilities of students have great help.

在不等式的等价转化过程中需要用到诸多的数学思想，如分类讨论问题、整体换元、数形结合、转化化归等等适当地渗透这些思想方法，对提高学生的数学能力有极大的帮助。

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

This suggested there might have cis-acting element in 3'-UTR. In order to search the possible trans-acting factor, we found RNA-protein band in H〓 cells and no band in 95D cells using 3'-UTR probe by RNA EMSA assay. At the same time, this band could be competed with the unlabled probe. There was also no band obsearved using 5'-UTR probe in both cells.

为了寻找可能与3'-UTR结合的蛋白质反式作用因子，用RNA凝胶阻滞(electrophoresis mobility shift assay，EMSA)的方法，我们在H〓细胞的胞浆蛋白质提取物与3'-UTR探针的结合反应中，发现了一条RNA-蛋白质的结合带，而在95D细胞没有结合带出现；而且这种结合带能被未标记探针竞争性抑制；5'-UTR的结合反应也没有观测到结合带的形成。

Radiolabeled pharmacological binding assay showed: 1 BmK AS bound specifically to a single class of non-interacting binding sites on both rat brain and cockroach nerve cord synaptosomes with high affinity and low low capacity. The association of BmK AS with both synaptosomes was quick to reach equilibrium, but the dissociation seemed to be only partial. The specific binding of BmK AS was independent of membrane potential, and couldn't be modified by veratridine. Moreover, the binding of BmK AS could be inhibited by native BmK AS, BmK AS1, BmK IT2 and BmK IT on both synaptosomes, but not or partially inhibited by BmK Ⅰ and suggesting BmK AS type neurotoxins might bind to a distinct receptor site on mammal and insect sodium channels with a similar manner from β-scorpion toxins.

二。蝎毒素多肽配体与哺乳动物和昆虫靶离子通道受体的药理结合特性及其靶结合位点的研究：经放射性标记结合和竞争抑制实验表明：1）BmK AS在大鼠脑和蟑螂神经索突触体上有单一非协同性高亲和力低结合容量结合位点，能快速结合并达到平衡，但仅能部分解离，其结合不依赖于膜电位，也不受藜芦碱调节，天然的BmK AS，BmK AS-1，BmK IT2和BmK IT可抑制〓AS与两种突触体的特异结合，而BmK Ⅰ无或仅有部分抑制效应。

These results indicated that palmatine had one common binding site on the immobilizedβ_2-AR column,the association constant was 2.93×10~4 L/mol at pH 7.2 and 25℃,while jatrorrhizine had two bingding sites,the association constants at these two sites were 1.93×10~4 and 1.56×10~5 L/mol,respectively.And the molar ratio of weak bingding sites to strong binding sites was 93:7.Palmatine and jatrorrhizine had competed for low-affinity site in immobilizedβ_2-AR.

结果表明，盐酸巴马汀与β_2-AR只有一类结合位点，在流动相pH 7.2，温度25℃时盐酸巴马汀与β_2-AR的结合常数为2.93×10~4 L/mol；盐酸药根碱与β_2-AR有两类结合位点，在这两类结合位点上的结合常数分别为1.93×10~4和1.56x10~5L/mol，两类结合位点数的比值为93：7，且盐酸巴马汀和盐酸药根碱竞争低亲和力的结合位点。

It was found that La〓 does not affect the binding affinity between calmodulin and Polistes Mastoparan, a known calmodulin binding peptide, neither the conformation of the ternary complex. Excessive amount of La〓 result in the decrease of the binding constant and the disrupted conformation. The binding affinity of La〓 to calmodulin increased in the ternary complex (La〓-CaM-Mas/Mas X), which suggested the coordination between the two global domains. The binding priority between the two global domains is also changed: La〓 more is likely to bind to the C-terminal of calmodulin than to N-terminal, thus facilitates the binding of Mas/Mas X to the C-terminal of calmodulin as the first step of the Mas/Mas X binding.

在以钙调蛋白结合肽—Polistes Mastoparan和Mastoparan X为对象的研究中，我们发现，除非过量，否则La〓的存在不影响钙调蛋白与Mas间的结合常数以及构象；过量的La〓（La〓/CaM摩尔比＞4）将引起钙调蛋白结合功能的下降，同时明显改变金属-钙调蛋白-Mas三元复合物的构象；La〓对Ca〓CaM-Mas的N末端表现高度选择性，显示了在三元复合物中两种离子间的协同效应；在三元体系中，La〓与钙调蛋白的亲和力明显上升，而且亲和力上升的程度因钙调蛋白结合肽的不同而有明显差异，显示了作用的选择性；Mas和MasX的存在改变了La〓在钙调蛋白上的结合顺序，La〓很可能优先与C末端结合，从而使Mas/Mas X首先与C末端结合，该顺序与钙离子相同；La〓的参与使三元复合物在动力学上更加稳定。

From such two levels as the history and logic of forming and developing Marxist educational economical thoughts, and practice and exploration of reforming and developing China's socialist enterprise, the chapter makes a systematic and deepened research and hackling of Marxist educational economical thoughts and their Sinicised theories, makes a profound investigation on the logical relationships between the reform and development of socialist educational enterprise, economic construction, and social progress, proves the scientificity of the judgement of " education has a leading and overall roles in modem construction" in a scientific way, and by combining with the hackling and researches of Jiang Zemin's educational economical thoughts, puts forwards such several important principles as converting the economic construction into the orbit of depending on the progress of science and technology, and raising the diathesis of the workers, insisting on the prior development of educational enterprise, insisting on overally implementing the strategy of science and education make a country stronger, pushing forward the two basic changes of educational turns, continuously deepening the educational reform, promoting education development, and combining with the progress of the economic society.

第二章 教育要与生产劳动和社会实践相结合从梳理分析马克思主义关于教育与生产劳动相结合思想的形成与发展及其实质与意义入手，论证了现代教育坚持与生产劳动和社会实践相结合的重要意义，考察了毛泽东、邓小平、江泽民等三代领导核心在中国革命和社会主义现代化建设的不同历史阶段对马克思主义教育与生产劳动相结合原理的创新与发展，对实行教育与生产劳动相结合的内容、原则、途径和方式等进行了全面阐述，并结合对江泽民有关论述的研究，提炼出教育与生产劳动相结合是坚持社会主义教育方向的根本措施，教育与社会实践相结合是当代青年成长的必由之路，推进经科教、产学研一体化是教育适应经济社会发展的崭新途径、适应经济发展和社会进步是教育与生产劳动相结合的本质等结论。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。