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Pox formation increased from 13.3%-20% to 33.3%-40% after passaging through 9 days old chicken embryo. Typical virus particles were observed under transmission electronic microscope in ultrathin sections of pathological tissues, allantochorion membrane of chicken embryos and affected cells.

用待检病料感染的BHK-21细胞培养物接种9日龄鸡胚绒毛尿囊膜,随着传代次数的增加,痘斑病变的出现率从13.3%~20%上升至33.3%~40%;用山羊痘病变皮肤、鸡胚绒毛尿囊膜和感染细胞进行超薄切片,在电子显微镜下可以观察到典型的山羊痘病毒粒子。

When large quantities of viruses are required for vaccine production or chemical studies, the allantoic sac is utilized.

如绒毛尿囊膜是常用的接种部位,用于1、病毒滴定2、病毒形态学研究3、病毒鉴定,几种病毒能在绒毛尿囊膜上产生水泡样损伤,称为痘痕。

METHODS: The different combinations of the biofilm were put on the surface of chick embryo chorioallantoic membrane.

将制备的生物膜置于正对观察窗的鸡胚绒毛尿囊膜表面的血管最少处,然后贴在鸡胚绒毛尿囊膜表面上,封口膜封闭假气室,继续孵育。

Through upregulating the production of MMPs in deciduas and chorionic villi, the upregulation of the expression of EMMPRIN in syncytiotrophoblast and cytotrophoblast column of the villi in the threatened abortion can degenerate deciduas and denudates embryo.

发生先兆流产时合体滋养细胞、细胞滋养层柱所表达的EMMPRIN增强可能通过上调绒毛及蜕膜组织中基质金属蛋白酶的水平,降解细胞外基质,使绒毛剥离、蜕膜组织剥脱。

Results: The normal epithelial cells were intactly lined with microvilli, and lesions on the surface of Ct infected cells were showed by SEM. The expression of actin was obvious throughout the cytoplasm of uterine epithelial cells but appeared to be most intense along the apical plasma membrane on day 4, 5 and 6 of pregnancy. On the day 7, levels of actin had increased along the apical surface. The expression of actin in the infected group was weaker than that in of the normal group.

结果:SEM示对照组子宫腔可见有微绒毛的分泌细胞;实验组腔上皮细胞表面有的可见破损,微绒毛少见;免疫组化结果显示荧光强度主要定位于腔上皮细胞胞质/胞膜,妊娠第4,5,6天沿胞质顶部分布较密集,而底部较弥散;妊娠第7天顶部强度有所增加;感染组的荧光强度均弱于相应正常组,在妊娠第5天存在显著性差异(p.05)。

The HBsAg was located in the intervillous space (21 placentas) and...

HBsAg主要分布于蜕膜血管内皮细胞(18例)、蜕膜细胞(21例)、滋养层细胞(8例)、绒毛间质细胞(8例)和绒毛血管内皮细胞(5例)的胞浆和绒毛间隙(21例)中。

HBV transplacental transmission route may be realized by cell to cell transfer in placenta, which means that HBVs in maternal blood and/or intervillous space infect DCEC and/or DC and/or TC, then transmit to VMC, then to VCEC, and finally to fetus.

HBV经胎盘感染胎儿的路径可能是通过胎盘细胞至细胞的传递而实现的。即母血和/或绒毛间隙HBV→蜕膜毛细胞管内皮细胞和/或蜕膜细胞和/或滋养层细胞→绒毛间质细胞→绒毛毛细血管内皮细胞→胎儿。

Correlation analysis indicated that total chlorophyll contents in leaves of F. velutina and R. pseudoacacia Seedlings were negatively correlated with MDA content under both neutral salt stress and mixed salt stress. The total chlorophyll contents was positively correlated with Pro under neutral salt stress.However, the correlation was negative under mixed salt stress.

相关分析表明,两树种在中性盐和碱性盐胁迫下,总叶绿素含量与膜脂过氧化产物丙二醛含量呈负相关;中性盐胁迫下,刺槐和绒毛白蜡都与脯氨酸呈正相关,在碱性盐胁迫下则呈负相第3期武德等盐碱胁迫对刺槐和绒毛白蜡叶片叶绿素含量的影响

The villus and deciduas were collected at the third day of medical abortion or at the day of dilatation and curettage.

两药物流产组取服药(米非司酮配伍米索前列醇)第3天排出的绒毛和蜕膜组织,对照组取负压吸引出的绒毛和蜕膜组织。

Results of the experimental groups: Comparison with the control groups, in 6h and 8h groups eminences of ependymal cells with cobblestone-like appearance were seen, their microvilli were further richer, and a number of microholes could be found on their base. In body part of SFO cell membrane of some ependymal cells were broken completely, their cytoplasm was no longer seen, and cell nucleus located at one side of the cell, 5-7μm in diameter and dividing lobe-like in shape. Beside those cells some ependymal cells appeared many folds on the top of surface. In 2h and 16h groups the observation results seemed no significant differences from the corresponding control groups.

实验组结果:与对照组相比,在6小时和8小时实验组中,可见成团的鹅卵石状室管细胞隆起于表面,微绒毛更为丰富,微绒毛根部可见到若干微细小孔,在SFO体部的一些室管膜细胞的胞膜完全破裂,胞质几乎荡然无存,仅剩胞核居于细胞的一侧,直径有5.7μm,呈分裂叶状;与这些细胞邻近的室管膜细胞表面最高处多呈现皱襞。2小时与16小时的实验组的观察结果与对照组相比未发现明显差异。

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