经证实的

On the functional differentiation,we found and that the type II ras-GTPaseactivating protein (p100-GAP),which was specifically expressed in humanplacenta,was largely expressed in the highly-differentiated syncytiotrophoblastcells and moderately expressed in the cultured intermediate trophoblast cells.While in the non-differentiated cytotrophoblast cell line-NPC,no expression was observed.Its mRNA and protein expressingcharacterization was in consistent with that of hCGβ,which was one of the mostimportant markers of the trophoblast cell functional differentiation.In addition,the expressing amount of p100-GAP increased with the progressing of pregnancyand the syncytium formation in vitro.

细胞功能分化的研究结果表明：经重组质粒的构建获得特异表达于人胎盘的p100－GAP的cDNA探针后，从蛋白和分子水平证实p100－GAP大量表达于分化程度高的合体滋养层细胞，少量表达于体外培养的中间型滋养层细胞，而非分化的细胞滋养层细胞NPC则不表达，这种表达特性与作为滋养层细胞功能分化重要指标之一的hCGβ高度一致；p100-GAP表达量还随妊娠过程和体外合体化过程而逐渐增加。

Maleic rosin has been added to the surface of wood flour in the form of monoester, i.e., the formation of esterified wood flour bearing a pendent carboxylic group. The rheological study showed that the complex viscosity η(superscript *, storage modulus and loss modulus of WF-HDPE composites decreased firstly and increased afterward and declined lastly as the dosage of maleic rosin increased, however, the viscosity of the composite melts decreased as a whole. After the modification of wood flour, the rheological properties of composite melts were improved markedly. According to the mechanical test it was indicated that, by proper treatment of wood flour with maleic rosin, the tensile strength and flexural strength of WF-HDPE increased obviously.

WF-HDPE流变学研究表明：复合材料的复合黏度η(上标*、储能模量和损耗模量随马来松香用量的增加先减小后增大最后减小，但体系的黏度和模量总体呈下降趋势，木粉经马来松香改性后复合熔体的流变性能显著改善，这不仅有利于提高复合材料的成型加工效率，而且适当的马来松香处理也能够提高复合材料的拉伸强度和弯曲强度，后者被静态力学试验结果所证实。

Therefore, Dr. Ciechomska and her coinvestigators wanted to see if ultrasound would accurately identify the features of nerve compression in patients with CTS. They obtained ultrasound images of 126 wrists in 52 patients who had been diagnosed as having CTS by EMG. They also obtained ultrasound images of the wrists of 22 healthy people. They used the Aspen unit (Acuson, Aliso Viejo, California), which was equipped with a linear, wideband transducer at 6 to 11 MHz.

Ciechomska和她的研究同僚于是对超音波进行研究，确定超音波是否可以正确的侦测出腕隧道症候群患者受到压迫的神经；研究人员从52位患者处获取了126个手腕超音波造影，这些患者皆经肌电图证实患有腕隧道症候群；同时，研究人员也取得了22位健康者的腕部超音波造影以为对照；所使用的仪器为Aspen (Acuson公司，位于加州Aliso Viejo市)，配备一线性的宽频电能转换器，频率设定在6到11 MHz。

Bone marrow containing hematopoietic stem cells has already been known , theses another group stem cells exists in bone marrow named mesenchymal stem cells. MSCs retain the potentiality of differentiating into osteoblast, chondroblast, adipoblast, tendonblast, myoblast, cardiomyoblast, neuron, etc , which displays a bright prosperity of its use in tissue enigeering, wound healing , cell transplatation and gene therapy. MSCs can be obtained and expanded easily compared to ESC without any problems of morality , law and ethic , immunology.

最早在130年前，德国病理学家Cohnheim在研究创口愈合时就推测在创口愈合过程中出现的部分细胞可能来自骨髓。20世纪70年代，Friendenstein发现一小部分骨髓中贴壁最牢固的细胞经数此传代后形成独特的纺锤形外观，具有形成成骨细胞或软骨细胞的能力。80年代以来，许多研究小组进一步证实了Friendenstein分离到的这些细胞是多能的，在体外特定诱导条件下，可以分化为成骨细胞、软骨细胞、脂肪细胞、成肌细胞、神经元等，被称为骨髓间充质干细胞(Bone MarrowMesenchymal Stem Cells，MSCs)。

Methods: Immunohistochemistry and image analysis techniques are applied to examine the phosphorylated extracellular signal－regulated kinase in epidermis of non－expanded skin and expanded skin. Results:Phosphorylated ERK expressed in basal cell layer of epiderm including non－expanded skin and expanded skin are expressed. But in expanded skin they are expressed significantly and densely compared to non－expanded skin. There are significant difference in relative to location of expanded skin and regions of expanded skin.

结果： 1扩张皮肤和正常皮肤表皮基底层都有磷酸化ERK 的表达和分布，但扩张皮肤中的磷酸化ERK的分布和表达较明显，染色较深且密，部分阳性细胞呈多层排列，有散在的增殖团区；2扩张皮肤的顶部和侧部差别不十分明显，而侧部和顶部与基底部有一定的差别，基底部染色较深，多为细胞核着色；3 经图像分析不同部位的正常皮肤和扩张皮肤以及扩张皮肤的不同部分的相对灰度值和阳性细胞密度，进一步证实了上述观察结果。

E., on the instructions of the waterworks committee had prohibited the use of municipal water for purposes other than those of consumption (envisaging the possibility of recourse being had to the impotable water of the Grand and Royal canals as in 1893) particularly as the South Dublin Guardians, notwithstanding their ration of 15 gallons per day per pauper supplied through a 6 inch meter, had been convicted of a wastage of 20,000 gallons per night by a reading of their meter on the affirmation of the law agent of the corporation, Mr Ignatius Rice, solicitor, thereby acting to the detriment of another section of the public, selfsupporting taxpayers, solvent, sound.

都市监察官兼水道局技官、土木工程师斯潘塞。哈蒂奉水道局的指示（鉴于有可能会像一八九三年那样被迫利用大运河和皇家运河那不宜饮用的水），除了饮用外，下令一律禁止使用市里供应的自来水。尤其是南都柏林济贫院，尽管限定用六英寸的计量器，每个贫民每日配给十五加仑水，然而在市政府法律顾问、辩护律师伊格内修斯。赖斯的监督下，经查表证实，每夜要浪费两万加仑水，从而使院外的社会各阶层（也就是自费并有支付能力的纳税者们）蒙受损害。

objective to study the ct diagnosis of ureteral calculus at the distal end of ureter.methods 82 cases of ureteral caculus were examined by ct scanning.all cases were proved by clinical therapeutic results.results ureteral calculuses were showed as high density image in ct scanning.the ct values of the ureteral calculuses less than 7.5mm in diameter were beyond 83hu.the detection rate by ct was 96.3%.in all the 82 cases,ureteral with calculuses were found dilated and 69 cases with nephrohydrosis.in 13 cases with no nephrohydrosis,caculuses less than 3.8 mm in diameter were found.conclusion ct is the most valuable way to diagnose ureteral calculus at the lower end of ureter.appropriate methods of ct scanning are essential.

目的 回顾性分析输尿管膀胱入口处结石的ct诊断结果，评价其ct检查的临床应用价值。方法分析82例输尿管膀胱入口处结石的ct检查结果，所有病例均经尿道排石证实。结果输尿管膀胱入口处结石具有特征性的ct表现，即圆形或枣核状钙化高密度影，结石直径＜7.5mm，ct值≥83hu，ct确诊率96.3%。82例均发现病侧输尿管增粗，69例发现病侧肾盂少量积水，肾盂未见积水的输尿管膀胱入口处结石13例，其结石直径＜3.8mm。结论 ct检查输尿管膀胱入口处结石具有准确、无创、直观等优点，是诊断输尿管膀胱入口处结石不可替代的检查方法。

Based on the above experiments, the author studied the mixed metallic pillared and modified montmorillonites. The experimental result indicated that the microporous structure of Fe-Al-PLMs was affected by the changing of interlayer Fe-Al polymer with different Fe/Al ratio. But the presence of Al iron was helpful to incorporate Fe iron into interlayer of montmorillonites.. Introducing Zr helped Al-PLMs getting better larger spacing, thermal stability, surface area and pore volumes. Its successful to apply Zr-Al-PLMs to treat wastewater containing Cr iron. The experiment of modifying Al-PLMs by PVA showed that the order of adding starting materials (Al-pillaring solution, PVA, montmorillonite solution) was related to the property of Al-PLMs. The PVA as precursor was beneficial for Al to go into interlayer of the clay and to form larger basal spacing porous clay. The surface area and pore volumes of Al-PLMs increased mainly because of increasing the external surface area and mesoporous volumes after acid-activating. This conclusion confirmed that the microporous structure of pillaring clay depends upon the interlayer pillar.

实验结果表明：在Al-PLMs中引入Fe离子，不同Fe／Al比制得的Fe-Al-PLMs因层间的Fe-Al聚合离子结构不同而影响其微孔结构，但羟基Al离子的存在有利于Fe离子进入蒙脱石层间；在Al-PLMs中引入Zr离子，有助于其层间距、比表面积、孔体积增大，所得Zr-Al-PLMs应用于含Cr废水处理效果显著；PVA改性后的Al-PLMs其物化性质变化与铝柱化剂、PVA溶液、蒙脱石溶液三者不同添加顺序有关，说明PVA的前撑有利于Al进入蒙脱石层间得到较大层间距的Al-PLMs；经酸化处理得Al-acid-PLMs，其比表面积增大以外表面积为主，孔体积增大以中孔为主，这也证实了柱撑蒙脱石的微孔结构取决于其层间的柱化物。

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法：利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变，使编码子249由精氨酸变成丝氨酸，然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中，将注射过的囊胚植入假孕的雌性小鼠子宫，到第14d取小鼠胚胎纤维母细胞，用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞，经测序证实细胞含有由249Arg到Ser的突变。

The objectives of this study are:(1) To amplify Tb wbbL gene encoding rhamnosyl transferase bypolymerase chain reaction from the genomic DNA ofM.tuberculosis H37RV strain;(2) To clone PCR product of Tb wbbLgene into a cloning vector pMD18-T for sequencing;(3) To subcloneTb wbbL gene to an expression vector pET16b to construct pET16b-Tb wbbL; and to overexpress Tb WbbL protein in E.coli BL21(DE3)under different induction conditions;(4) To establish theco-expression system for expressing chaperons of pKJE7 plasmidand soluble Tb WbbL protein in E.coli BL21(DE3) under differentinduction conditions;(5) To test expressed WbbL protein by SDS-PAGEand Western blot methods.

本论文的目的是：(1)利用 PCR 方法从结核分枝杆菌 H37Rv 菌株的基因组DNA 中扩增出 Tb wbbL 基因；(2)将 Tb wbbL 基因克隆到pMD18-T 克隆载体中，经 DNA 序列测定证实为正确的基因；(3)将 Tb wbbL 基因亚克隆到 pET16b 表达载体中并通过改变不同的诱导条件在大肠杆菌 BL21(DE3)中表达 Tb WbbL 蛋白质；(4)建立在 BL21(DE3)大肠杆菌中共表达 Tb WbbL 蛋白质与分子伴侣的体系，优化表达条件以高效表达出可溶性 Tb WbbL蛋白质；(5)用 Western blot 方法鉴定所表达的 Tb WbbL 蛋白质为 Tb wbbL 基因产物。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了