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Results: GINaTK retroviral vector containing HSV-tk was constructed and transduced into PA317 retrovirus packaging cell by lipofectin (named PA317TK). Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P <0.001.

结果:构建了带有HSV-tk基因的反转录病毒载体GINaTK,应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317,成为产病毒细胞PA317TK,用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞,命名为C6TK细胞,对GCV和ACV的敏感性分别高于亲本450倍和10倍;成功地建立了大鼠脑胶质瘤模型,并证实转染细胞C6TK的成瘤效应未改变,存活期约为15天;而经ACV治疗后,含有C6TK细胞的肿瘤生长明显被抑制,大鼠生存期延长为57.8±8.07天,尤其是采用PA317TK细胞混和治疗组和原位治疗组,肿瘤基本消失,大鼠生存期显著延长,混和治疗组存活120天以上,原位治疗组存活至71.4±36.1天。t检验,P值均小于0.001。

Methods Six cadaveric lumbar vertebrae were harvested and evaluated with dual-energy x-ray absorptiometry and quantitative computed tomography to assess bone mineral density. Matched, polyaxial pedicle screws were then randomly assigned by side and placed using the Magerl, Krag or caudad trajectory technique assisted by Iso-C 3D scan and 3D navigation system. Cyclic loading tests were performed with a maximum 250 N load at 3 Hz up to 30000 cycles while recording load and displacement. Stiffness quotients were calculated it comparison.

从尸体中获取6节腰椎椎体经DEXA和QCT检测骨密度值,证实为骨质疏松后在计算机导航下按随机分组的方法向12个椎弓根中置人不同方向的椎弓根螺钉,使用MTS-858生物力学仪脊柱力学测试平台进行±250N的循环负荷疲劳测试,记录负荷循环中的螺钉位移,计算系统的刚度系数并进行对比分析。

A dash of Maraschino makes the perfect daiquiri, as confirmed by daiquiri-making legend Constantino Ribalaigua of La Floridita in Cuba, reputed to have made over a million of those drinks in his lifetime .

古巴调制黛克瑞的传奇人物Constantino Ribalaigua ,在他的有生之年,调制出了超过1万杯的饮品,经他证实,少量野樱桃利口酒就能调出一杯完美的黛克瑞。

To explore the functions of SPANXA1 in cancerous phenotypes CL1-5 cells were transfected with a SPANXA1 expressing vector and evaluated by cell proliferation, cell migration, Matrigel invasion and colony formation assays in vitro as well as mouse metastasis assays in vivo. The results indicated that the induction of SPANXA1 could reduce cell invasiveness. In the other hand, immunostaining showed that SPANXA1 was predominately located at the nucleoplasm.

经RT-PCR验证得知SPANXA1在CL1-0的表现量比CL1-5多100倍以上,於是我们将SPANXA1转染在CL1-5细胞株中,并测量活体外的细胞生长速度试验、细胞迁徙实验、Matrigel侵袭实验、癌细胞群落形成实验及小鼠活体内细胞转移能力,探讨SPANXA1对癌细胞性状的影响,结果发现增加SPANXA1会降低癌细胞的转移能力,在另一方面,我们利用免疫萤光染色法侦测出SPANXA1是存在於核质中,并以西方点墨法再次证实SPANXA1是存在於核质中。

Approved the substitution effect of 2, 2-diphenylnaphthalinspyran on the phenyl ring, and clarified the property of the compound is not determined only by the pyran planarity and C-O bond, it not only changed the theoretic basis, but also provide a way to find materials with new properties.

经单晶分析证实了2,2-二苯基萘并吡喃的苯基上取代基的种类及其位置对光致变色性质的重要影响,改变了只有吡喃平面性及其C-O影响性质的观点,不仅改变了理论依据,还指导有望开发出新性质材料的方向。

WITHOUT here entering on a discussion of the doctrine of Angels of devils as present in Holy Scriptures, the Apocrypha, and the Pseudepigrapha, it will be admitted that considerable progression may be marked as we advance from even the latest Canonical to Apocryphal, and again from these to the Pseudepigraphic Writings.

但在这里进入了讨论的理论,天使作为当前德弗尔斯圣经中的伪经,以及Pseudepigrapha ,这将是承认,相当进展可能会被标记,我们事先甚至最新的典型,以未经证实,并再次从这些著作的

Polyethylene glycol (MW6000 35%)-Ca〓(40mmol/l) system was applied to research on the fusion of nuclei and anucleated protoplasts which were deactivated by ultraviolet rays. The fusion process was verified by -dCTP radioisotope tracing with radioautography.

选择PEG6000促融,40mmol/1Ca〓进行了K氏酵母细胞核与His酵母经紫外线灭活的无核原生质体融合的研究,利用放射性同位素dCTP的示踪结合放射自显影技术,对融合进行检测,证实了融合的确实发生。

In the application, the signal of refection is strong and the buried faults can be reflected evidently.

所给实例中的反射波组能量强,隐伏断层的反应明显,经跨断点钻探验证,结果证实地震勘探确定的界面深度、断点位置和基岩错距等参数都是可靠的。

We choose 20 cases of fungal rhinosinusitis who were treated in our department between the year 2007.4 and 2008.4 as our study group and 5 cases of purely nasal septum deviation as control group. The 20 cases contain 19 cases of fungus ball and 1 case of chronic invasive fungal rhinosinusitis. Their age fluctuate from 19 to 80, and the average age is 51 . All of the 25 cases blood samples were taken to detect the 1-3-β-D glucosan with the kit produced by Beijing jinshanchuan technology company and under the MB—80 quickly microorganismal detection system.

采用北京金山川科技公司生产的试剂盒及MB—80微生物快速检测系统分别检测中国医科大学附属盛京医院耳鼻喉科于2007年4月至2008年2月经鼻窦内窥镜手术治疗,并经术后病理证实为真菌性鼻窦炎患者(19例为真菌球型,1例为慢性侵袭性真菌性鼻窦炎)的血液样品20例,患者年龄19岁—80岁,平均年龄51岁,采取中国医科大学附属盛京医院耳鼻喉科于2008年1月住院单纯鼻中隔偏曲患者的血液样品5例作为对照组,患者年龄16—56岁,平均年龄32岁(以上血样标本的采集患者均已签署知情同意书)。

In the original studies,[4,5] all the acute stroke patients with angiographically proven internal carotid artery or M1 occlusion had ALN, presumably because the lentiform nucleus is fed by lenticulostriate arteries from M1 without collateral flow from cortical anastomoses.

先前的研究报道:所有经脑血管造影证实存在颈内动脉闭塞或大脑中动脉M1段闭塞的急性卒中患者,都会出现豆状核低信号征;可能系豆状核是由大脑中动脉M1段发出的豆纹动脉供血,缺乏来源于皮层吻合支的侧枝循环供血所致。

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Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时,他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

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世纪伟人邓小平的富民思想,是邓小平经济理论体系中的重要组成部分,是我国经济建设根本任务的一个重要方面。