经皮的

The antibodies were purified and the purity and specificity were identified by SDS-PAGE and Western-blot,respectively.Lung tissues from five pigs with PRRS were tested with the established method,which clearly demonstrated the abundant presence of viral antigens in the cytoplasm of alveolar macrophages,and to less extent in alveolar and bronchiole epithelial cells.

SDS-PAGE和Western-blotting检测结果表明，制得的兔抗PRRSV IgG纯度较高，具有良好的特异性，可用于原位检测病猪肺组织中PRRSV抗原的分布；Envision法的检测结果显示，PRRSV抗原的阳性表达产物主要出现在肺巨噬细胞胞浆内，其次为肺泡上皮细胞和细支气管黏膜上皮细胞；通过对5份经RT-PCR检测确认的PRRS病猪肺组织进行检测，均有较高的阳性细胞表达，平均阳性细胞率为46.5%。

Series is divided into knitted fabrics, woven two major categories, mainly a variety of mesh cloth, Wen Zhangbu, velveteen, mercerizing velvet, gold velvet, velvet, for the cloth, polyester cotton cover, rib and Chinese cloth, double-sided fabric, Flannel, fleece, ants cloth, beads and mesh fabrics, elastic cloth, jeanette Tasi cashmere, spent shake, Chun Yafang, Dita Fu 210 T, 190T, 180T, 170T, all kinds of knitted, woven composite Cloth, leather, PVC, PU, bath towels cloth, special cloth, and so on.

布料系列分为针织、梭织两大类；主要有各种网眼布、蚊帐布、平绒、丝光绒、金光绒、天鹅绒、经编布、涤盖棉、罗纹、汉布、双面布、绒布、摇粒绒、蚂蚁布、珠地网眼布、弹力布、桃皮绒、塔丝绒、花摇、春亚纺、涤塔夫210T、190T、180T、170T、各种针织、梭织的复合布、人造革、PVC、 PU、澡巾布，特种布等等。

Also cavity in the elytra was found, which are supported by pier formed pillars formed by integument sinking for supporting and reducing weight.

膜翅的背腹壁之间也具有内腔和皮细胞，并充满油脂腺(经分析是棕脂肪细胞腺)及其分泌物。

The streptomycin perfusion of labyrinth via a fenestra of LSC could treat disabling Meniere's disease and other dizziness caused by inner ear disorder by selectively destroying the sensory epithelium of utricular macula and three canal cristae.

经水平半规管开窗链霉素迷路灌注术可以通过选择性破坏椭圆囊斑和三个半规管腹壶嵴感觉上皮根治难治性梅尼埃病和其它内耳疾病引起的眩晕或头昏。

About the Calculation of Arc length of the Oriented Smoth Curve or Jordan;2. The experimental results show that the optimum melting voltage is 35～36 V,the reasonable arc length is 25～29 mm.

以海绵钛和中间合金压制的电极经两次熔化得到的TC11合金铸锭为原材料，采用真空自耗电弧炉，在不同的电压下熔炼3次TC11合金铸锭，研究熔化电压、弧长与TC11合金铸锭扒皮深度和扒皮量的关系。

Concept of leakage coefficient of rod pump and amendment of its calculating method

性质：为修饰改善原料皮及加工中造成的粒面缺陷，通过磨革磨去革面较浅的伤残，经涂饰和压成假粒面，建立一个新的表面，经这样修饰的面革称为修正面革。

It indicates that the raw materials can be stored in temperature of 0-4℃ for 60 days, they must be immersed in clean water for more than one hour before processing, and peeling be carried out in flowing water; that color preserving solution should be 1.0% NaCl+0.2% citric acid+0.5% Vitamin C+110ppm dichloro isocyanuric acid sodium; that combined browning inhibiting solution is conspicuously effective, and immersion time better be around 20 minutes; that sterilization solution ought to be 200ppm dichloro isocyanuric acid sodium+0.5% ascorbic acid+0.3 citric acid, and 10 minutes' immersion will achieve the effect of sterilization and inhibiting browning; and that after 20-21 minutes of quick-freezing in temperature of -31℃~-32℃, the products can be preserved under -18℃ for a whole year.

通过对速冻牛蒡加工过程中原料储存、削皮方法、护色、浸泡时间、无热杀菌、包装形式等参数进行研究表明：原料0-4℃可储存60天，加工前用清水浸泡1小时以上，削皮时在流水中进行，护色液筛选的1.0% NaCl＋0.2%柠檬酸＋0.5%维生素C十110ppm二氯异氰脲酸钠；复合褐变抑制溶液在抑制褐变方面有显著的效果，浸泡处理时间为20分钟为宜；杀菌液为200ppm二氯异氰脲酸钠＋0.5%抗坏血酸＋0.3柠檬酸，浸泡10分钟即可达到灭菌和抑制褐变的作用；经-31℃~-32℃温度下速冻20~21分钟，产品在-18℃条件下可储存一年。

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果：倒置显微镜和透射电镜观察显示，经SAHA处理的细胞增殖速度显著减慢，细胞体积增大，细胞核较小，形状较为规则，核仁数量减少、体积变小，核内常染色质增多而异染色质减少，核质比例减小，细胞质内线粒体数量增多、线粒体嵴发达，高尔基体较为典型，粗糙型内质网增多，呈现出与正常上皮细胞相似的形态变化；MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用，并有明显的剂量依赖和时间依赖关系；免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达；流式细胞仪检测结果显示，SMMC-7721细胞经SAHA处理后，G0/G1期细胞明显增加，S期细胞则明显减少，细胞被阻滞于G0/G1期；RT-PCR检测结果表明，SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加，24h后更为明显。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！