经用

The sample was then rinsed with 10% HF + 40 % HNO3 + 50% H2O, ultrasonic-cleared in acetone, dried, oxygenized in H2O2, rinsed, dried, sterilized, and lixiviated. L-929 cells were cultured in vitro and assigned into negative control, surface oxidation (surface oxidation + NiTi-SMA leaching liquor), and non-treatment groups (NiTi-SMA leaching liquor alone).

用10%HF+40%HNO3+50%H2O进行酸洗，丙酮中超声波清洗，干燥，在H2O2溶液中氧化，再经清洗、干燥、灭菌、浸提等处理。L-929细胞体外培养，实验分为阴性对照组：培养液培养；表面氧化组：面氧化处理NiTi-SMA浸提液培养；未处理组：未经处理NiTi-SMA浸提液培养。

The effects of different liquefaction time on DE value and the lixiviating rate of raw materials were investigated.

以国产大麦为原料，采用全酶法经预水解、液化和两段糖化制备酿造啤酒用大麦糖浆生产工艺。

Methods:DNA was extracted from tissues of HCC and amplified by PCR.MI was determined by using seven microsatellite markers and mutational ana lyses of IGFⅡR by running sequencing gel electrophoresis.

用QIAamp方法提取肝细胞癌DNA，选择7对引物，经PCR扩增，走DNA序列胶电泳，测定其MI的阳性率及有无IGFⅡR基因的突变。

When the temperature was at 25℃, the EFS30 OPS two-step method had a blastocyst rate of 62.22%, the highest rate in all groups. After the morulae cultured from thawed embryos from the best straw group and OPS group were transferred, all groups have obtained offspring.

在25℃温度条件下细管法和OPS二步法玻璃化冷冻后的小鼠2-细胞胚胎，经培养发育至囊胚后，用H33342染色，在荧光显微镜下观察并进行细胞计数。

BALB/C mice were immunized with the purified PrV of MIN A isolate,mouse spleen cell were fused with myelomas NSo.Culture supernatants of hybridomas were screened by indirect ELISA and immobilization ELISA,positive colonies were cloned three times by limited dilution.

用纯化的伪狂犬病病毒免疫BALB/C小鼠，取脾细胞与NS_0骨髓瘤细胞融合，经间接ELISA和固相化ELISA筛选，3次有限稀释法克隆，获得了1株能稳定分泌抗伪狂犬病病毒单克隆抗体杂交瘤细胞株，命名为OH_6。

Similarly, the way some singers "lead up" to a note, using a nasalized slur is a device passed in oral transmission from person to person, possibly influenced by traditional fiddle-playing technique.

同样，一些歌手用鼻音于音符间滑唱的方法，也是经口头流传下来的，可能是受到小提琴演奏技巧的影响。

Methods Rats were infected with MP for 9 times during a period of 24 weeks by ultrasonic nebulizing inhalation to establish animal models of chronic pulmonary MP infection. In situ hybridization was performed with bFGF cDNA probe and results were quantitatively analysed to measure the changes of bFGF mRNA expression in lung tissues.

将8只大鼠于24周内反复9次经超声雾化吸入肺炎支原体以复制其慢性肺部感染模型，另以4只为对照，随后用bFGF cDNA核酸探针行核酸分子原位杂交及定量图像分析，观察肺组织bFGF mRNA表达的变化。

METHODS: After Feulgen staining, 40 paraffin samples of astrocytic tumors were observed about DI and 5cER by image analysis, MRI appearances were reviewed, including edema around tumor, clearity of boundary, equality of MRI singal, enhancement of tumor, shift of medline, necroses and cyst, then compared them with pathological grade and survival rate.

40例星形细胞肿瘤石蜡标本经Feulgen染色后，用图像分析仪检测其DNA指数及超5倍体率(5cER)；复习患者MRI表现，包括：瘤周水肿程度、边界是否清楚、信号是否均匀、病灶是否明显强化、病灶是否过中线、有无囊变坏死6项指标，与肿瘤病理分级诊断及生存率相比较。

Methods: The teeth of 4 cases were treated by filling the perforations with the 3M ESPE Single Bond Adhesive and the 3M ESPE F200 Compomer through the pulp cavities,the root canals were obturated well and follow-up surveys were conducted for 3 months.

对4例医源性髓腔穿孔的患牙用3M SingleBond粘接剂和3M F200光固化复合体经髓腔充填修复穿孔，进行完善的根管充填后3个月随访观察。

According to the bactericidal assay of quantitative vehicle, the bacteria on the ozonized vehicles and unozonized vehicles was washed, 50μl eluant was seeded on the TSA plates.

用碘滴定法测定打开臭氧发生器不同机组及不同时间时的臭氧浓度，按载体定量杀菌试验，将经臭氧作用的载体与相同环境下未经臭氧作用的载体上的细菌洗下，各取洗脱液50μl，接种于TSA平板并置厌氧箱（90％N2，10％CO2，37℃）培养24-48h后，进行活菌菌落计数。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。