经用

A study was conducted to synthesize oligoester alcohol s from poly production wastes via transesterification with diethylene glycol and tribasic alcohol.

将涤纶废产物用二甘醇和三元醇经酯基转移合成齐聚酯多元醇，并用其制备聚氨酯硬质泡沫塑料。

The recombinant pET-Lip vector was transformed into E.coliBL21 and induced to express by 1mmol/L IPTG at 37℃. An expected 80kDa fusion proteinwas expressed. SDS-PAGE analysis showed the fusion protein located in the supernatant ofbacteria lysate by sonication. The fusion protein was purified by HisTrap~ HP Kit and coulddegrade tributyrin.

该菌株经IPTG诱导可表达分子量约80 kDa的融合蛋白，SDS-PAGE分析表明融合蛋白位于菌体超声裂解后的上清中，用蛋白纯化试剂盒HisTrap~ HP纯化后得到单一的目的蛋白，约80 kDa，且纯化的融合蛋白具有脂酶活性，可以分解三丁酸甘油酯。

Urine samples were first precipitated with trichloroacetic acid, then centrifuged at 12000 r/min for 15 min at 4 ℃, and finally added with NaOH to neutralize the supernatant.

尿标本经三氯乙酸处理，在4 ℃下，以12000 r/min的速率离心15 min，上清液用碱中和后，取30 μL直接进样。

Methods 100 mg of muscle of rat corpse was preprocessed before trimethylamine was extracted with anhydro-methylbenzene, then trinitrophenol was added into extracts, and yellow compounds emerged. The optical density was detected by spectrophotometer at 410 nm and the concentration of TMA-N was calculated using the standard curve. Results There was good linear correlation (R^2=0.9991, P.001) between OD and the concentration of TMA-N in the range of 1~10 mg/L.

大鼠肌肉组织样本100 mg经预处理后，将三甲胺抽提于无水甲苯中，与苦味酸作用，形成黄色的苦味酸三甲胺盐，用分光光度计在410 nm处检测吸光度，标准曲线法计算TMA-N含量。

Using wheat variety Fukuho pollinated with Tripsacum dactyloides,fertilization and early embryo development were studied.

用石蜡制片法和整体解剖法，对小麦品种Fukuho经鸭茅状摩擦禾芬粉授粉后不同时间固定的子房样品进行了细胞胚胎学观察。

Magnesium trisilicate was synthesized by precipitation using Na2OnSiO2 and Mg (NO3)2 as raw materials.

由Na2OnSiO2和Mg(NO3)2经沉淀法合成了三硅酸镁，用450℃煅烧或酸化方法对合成的样品进行改性。

Methods Transmission turbidity and turbidimetric assay were used respectively to detect serum rheumatoid factors from 35 RA cases and 60 healthy controls and the results were analyzed by statistics.

用透射比浊法和散射比浊法检测35例RA患者和60例健康体检者血清RF值，经统计学处理进行分析比较。

Products are divided into cutting,缎档, spiral, jacquard, printing, embroidery, twistless, bamboo fiber several major series, all with high-quality lint yarn, Twistless Yarn, AB yarn by special refined form.

产品分为割绒，缎档，螺旋，提花，印花，绣花，无捻，竹纤维几大系列，均用优质棉绒纱，无捻纱，AB纱经特殊工艺精制而成。

It is a braided packing made of Kevlar yarns impregnated with P.T.F.E.dispersion and treated with lubricant,This packing can bear hither-pressure applications than other typees of braided packings ,Due to its steel-lide strength,this yarn can be employed separately or as back-up rings with other types of packing.

TW-203芳纶纤维盘根：它是同Kevlar纤维经浸渍四氟乙烯乳液及用润滑剂，处理再编织而的盘根，这种盘根比其它各种编织盘根能承担更高的介质压力，由于这种线像钢丝一样强，它可和其它种类盘根分隔安装或作为端面环。

When total protein was assayed by 2-D PAGE andstained with sliver, about 2000 protein spots with a pI of pH 3-10 and Mrof 12-120 kDa could be detected. All these protein spots were excised foridentification, among which 213 spots corresponding to 192 differentproteins were identified undoubtful by searching the mass spectra againstthe SwissProt/TrEMBL database.

我们利用双向凝胶电泳对小鼠卵巢蛋白进行了分离，在考马斯亮兰染色后将所有可见的蛋白点切取下，经胰蛋白酶消化以后用MALDI-TOF质谱仪进行鉴定。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。