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RESULTS: After differentiation of human adherent myoblasts by neural induction medium, no cells with a neural cell morphology (ie., small, refractile cell body with dendritic cell extensions) were seen. All remaining myoblasts cultured with neural induction medium, myoblasts with proliferation medium and myotubes with differentiation medium containing 20 mL/L HS were positive for β Tubulin Ⅲ. C2C12 myoblasts were negative for β Tubulin Ⅲ. In contrast, all the above cells were negative for the markers Neurofilament Mr 68×103 and GFAP.
结果:用诱导分化液作用后,未见小的、伴有突起的放射状形态的神经细胞;抗β Tubulin Ⅲ对经神经元胶质细胞诱导分化液作用后的人成肌细胞、增殖培养液培养后的各代人成肌细胞及仅含20 mL/L HS分化液分化形成的肌管细胞染色均为阳性; C2C12细胞β Tubulin Ⅲ抗体染色阴性;上述所有细胞抗Neurofilament Mr 68×103和抗GFAP染色均为阴性。
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Especially , the rearrangement reaction of converting styrene oxide to phenylacetyldehyde were investigated. In this paper, we also studied the method of preparing SO42-/TiO2 by vitriolic titanium boiling regurgitant hydrolyzation and applications of SO42-/TiO2 in the preparation of iso-amyl acetate.
对环氧苯乙烷经分子重排反应制备苯乙醛进行了深入研究;本论文还用硫酸钛沸腾回流水解法制备了纳米SO_4~(2-)/TiO_2固体超强酸粉末,并应用于冰乙酸与异戊醇酯化合成乙酸异戊酯的反应。
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Freehand has a cutting tool, the picture may not be used, a graphic image after relink will return to its original condition.
Freehand洋有一个切图片的工具,不克不及用,图形图像经功从头链接会恢复原状。
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Heteroduplex molecules have been made by mixing pJSA1175 and pJSAl175ADRV4, pJSA1175ADRV4 and pJSAl175RRV4 DNA molecules which have been linearized by Pst Ⅰ and Bgl Ⅱ respectively, denaturing them in alkaline or high temperature, allowing them to renature and then spreading in the presence of formamide.
以痘苗病毒表达载体pJSA1175及重组质粒pJSA1175ADRV4(含ADRV VP4基因)、pJSA1175RRV4(含猴轮状病毒VP4基因)为材料,用碱变性方法或热变性方法制备了载体质粒与重组质粒DNA之间的异源双链,电镜下成功观察到载体序列部分经变性、复性后配对呈双链状态,而外源基因区域突出呈单链环状结构,测量长度为2.3kb。
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The ultra-fine disperse black ECO was prepared from traditional disperse black ECO by ball milling, reprecipitation and pressure homogenization. The effects of ratio of dyes and milling media, milling time, ratio of dyes and organic assistants, etc on were investigated.
将普通分散染料黑ECO各组分按比例混合后加入复合分散剂及有机助剂,用球磨机高度分散后滴加到复合分散剂的水溶液中,最后经高压均质机均质后得到超细分散染料黑ECO液体染料。
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The dogs were administrated with carboplatin at the dosage of 16.5mg/kg intravenously, intra-arterially or retroperitoneally. The blood samples were taken at 0, 5, 15 minutes, and 0.5, 1, 2, 4, 8, 24 and 72 hours after instillation. The competent platinum concentrations were measured with high-performance liquid chromatography. Then the pharmacokinetic parameters were calculated and analysed.
随机将18只雌性家犬分为3组,分别经静脉,动脉及腹膜后推注卡铂(16.5 mg/kg),然后于给药后的0、5、15分钟,0.5、1、2、4、8、24、72小时取静脉血,用高效液相色谱法测定血浆活性铂的浓度,计算和比较不同给药方式下卡铂的药代动力学参数。
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A highly active hydrogen added catalytic nanometer Ru/C is made by reducing metal ruthenate, getting nanometer ruthenate metal glue under the protection of surface activator, filtering, washing glue solution drawing by the carrier to get highly scattered loading nanometer ruthenate metal catalyst.
本发明涉及一种高活性加氢催化剂纳米Ru/C的制备方法,该方法首先通过化学还原金属钌盐,制得表面活性剂保护下的纳米钌金属胶体,然后用载体吸附制得的胶体溶液,经过滤,洗涤,获得高度分散的负载型纳米钌金属催化剂。
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Methods:30 nomal subjects and 12 old myocardial in farction patients were inclade. The wall motion scove were analysed by experieced obsservers and less experienced obsevers and ck techniques.
分别对30例健康者及12例经冠脉造影证实为左冠状动脉狭窄≥75%的陈旧性心肌梗塞患者的左室各节段室壁运动状态用常规室壁运动分析法和CK技术进行超声评分。
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The yellow serofluid esterified fluid after the treatment such as precipitation and decoloration etc. was used directly in liquor blending, which could improve liquor quality and increase quality product rate and reduce greatly production cost.
将经沉淀、脱色等酯化技术处理后的黄浆水酯化液用来直接勾兑白酒,可提高酒的质量和优质品率,并可大幅度降低生产成本。
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β-Cyclodextrin with the 6-hydroxyls protected by dimethyl-tert- butylsilyl was selectively oxidized by lead tetraacetate. After the silyl groups were removed off by boron fluoride-etherate, mono-2,3- dialdehydro-β-cyclodextrin as a cuniform molecule could be constructed.
用四醋酸铅将七元(6-O-二甲基叔丁基硅基)-β-环糊精选择氧化、并用三氟化硼乙醚脱硅基保护基后,再经分离、纯化,可获得具有&楔筒状&空腔的单-2, 3-二醛基-β-环糊精。
- 推荐网络例句
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For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether
年美国个人破产法的一个改动使得破产登记急速下降,而后引起了信用卡大规模的亏损。
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Eph. 4:23 And that you be renewed in the spirit of your mind
弗四23 而在你们心思的灵里得以更新
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Lao Qiu is the Chairman of China Qiuyang Translation Group and the head master of the Confucius School. He has committed himself to the research and promotion of the classics of China.
老秋先生为中国秋阳翻译集团的董事长和孔子商学院的院长,致力于国学的研究和推广。