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Benzene homologues in air of workshops were preconcentrated with the activated charcoal sampling tube, and then desorbed with carbon disulfide.

用活性炭采样管富集车间空气中的苯系物,以二硫化碳为解吸剂,经毛细管柱GC MS选择离子法测定样品中苯系物,峰面积定

The results showed that, using transient GUS expression, the frequency of resistant calli and shoot differentiation as a criteria, wheat mature embryos were cultured on the preculture medium of CM4C1P for 14 days, and immersed in inoculation suspension Ⅰ for 3 hours, then the explants were co-cultured under desiccation conditions for 3 days, which was optimal conditions for Agrobacterium-mediated transformation of wheat mature embryos.

为了进一步完善根癌农杆菌介导的小麦遗传转化体系,以小麦成熟胚为转化受体,研究了根癌农杆菌介导法转化小麦的主要影响因素,结果表明,以gus基因瞬时表达率、抗性愈伤组织获得率和分化率为转化指标,小麦成熟胚在预培养基CM4C1P上预培养14d后,用侵染培养液Ⅰ侵染3h,再经干燥共培养方式处理3d,是根癌农杆菌介导成熟胚转化的合适条件。

Targets and methods: Shanghai psychological counseling centers for treatment in patients with SAD voluntary CBGT, meet DSM-IV diagnosis of SAD standards, sex irrespective of age; Records of the patients general information and history information, including advisory, name, sex, age, occupation, culture, marriage, family history, course of illness, age of onset, inducing factor, premorbid personality, grandfather, and so on and treatment of MMPI; participate in group therapy treatment of SAD during the treatment of SAD did not take drugs, If that is used to the drug subject to a two-week drug washout period. Six cases of sleep well at night before bed without taking regular small doses of drugs Zhumian stability.

对象和方法:上海市心理咨询中心就诊的SAD患者中自愿参加CBGT者,符合DSM-IV关于SAD的诊断标准,性别年龄不限;记录患者的一般资料及病史资料,包括咨询号、姓名、性别、年龄、职业、文化、婚姻、家族史、病程、起病年龄、诱发因素、病前性格、既往治疗情况等及MMPI测试;参加集体治疗的SAD者治疗期间均未服用治疗SAD药物,如即往用过药者需经两周的药物清洗期,其中6例因睡眠不好于夜间睡前不定时服用小剂量安定类药物助眠。

The high vegetable, the seed introduces from Japan, becomes by seven kind of fine vegetables after the high tech hybrid, in the Zhuji mountainous area planter, spring harvests, does not have the insect pest, the pesticide pollution pure natural green agricultural product, this product uses the Japanese craft protosalt lactic fermentation the unique facture, carries on the processing souse turmeric yellow with the plant for the primary data, both maintained the salt has soaked the vegetables color, smell and tastes, and to include many kinds to the human body beneficial amino acid, this product had the natural flavor, tender, crisply in a body, ate the law to be diverse, might also make the seasoning, might also open package of edible, was the environmental protection, the health organic foods.

高菜,种子从日本引进,由七种优良蔬菜经高科技杂交而成,在诸暨市山区种植,春季收获,无虫害、农药污染的纯天然绿色农产品,本产品采用日本工艺低盐乳酸发酵的独特制法,用植物姜黄为主要原料进行加工腌制,既保持了腌渍蔬菜的色、香、味、又含有多种对人体有益的氨基酸,本产品具有天然风味,嫩、脆于一体,吃法多样,既可作佐料,又可开包食用,是环保、健康的绿色食品。

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法:利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变,使编码子249由精氨酸变成丝氨酸,然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中,将注射过的囊胚植入假孕的雌性小鼠子宫,到第14d取小鼠胚胎纤维母细胞,用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞,经测序证实细胞含有由249Arg到Ser的突变。

Standard enthalpy changes of the initial pyrolysis process of ethylbenzene were computed by five methods (UAM1, UHF/3-21G*, UB3LYP/3-21G* and UB3LYP/6-31G*). UB3LYP/6-31G* due to its accuracy compared to the experimental values was chosen to calculate the thermodynamic and kinetic parameters of the process at different temperature ranges (298 ~ 1573 K). The computation results showed that ethylbenzene began to pyrolyze at 973 K and the main product was styrene.

对乙苯的初期热裂解过程,用三种方法结合两种基组(UAM1、UHF/3-21G~*、UB3LYP/3-21G~*和UB3LYP/6-31G~*)计算了标准反应焓变,经与实验值进行对比,筛选出计算结果精确的UB3LYP/6-31G~*方法,并以此获得了乙苯初期热裂解过程在298~1573 K范围内的热、动力学参数。

Personality traits were evaluated by Eysency Personality Questionnaire that was compiled by Eysency and revised by Gong. Standard scores of Psychoticism, Extraversion and Neuroticism were calculated, and taking 50 points as a boundary. Each dimension was assigned into 2 grades non-psychotic type (P0) psychotic type (P≥50), introversion type (E0), extraversion type (E0), non-nervous type (N0) and nervous type (N≥50). 5 mL ulnar vein blood was obtained without adding decoagulant, and then serum was removed. Genome DNA was extracted from blood clot by conventional chloroform for PCR analysis. PCR product was sequenced by pyrophosphoric acid. SNP was determined by PSQ96 MA sequenator. Allele frequency was analyzed by mass spectrometry.

人格评定采用Eysency编制,龚耀先修订的艾森克人格问卷、计算精神质,内外问,神经质3个个性维度的标准分,并以50分为界,将各维度分为两个等级:非精神病倾向型(精神质0)、精神病倾向型(精神质≥50);内倾型(内外向0),外倾型(内外向≥多50);非常经质倾向型(神经质0),神经质倾向型(神经质≥50)取肘静脉血5 mL,不加抗凝剂,弃血清,血凝块常规酚氯仿提取基因组DNA,进行PCR分析PCR产物应用焦磷酸测序PSQ96 MA测序仪进行SNP检测,用质谱法进行及等位方基因频率分析。

the title compound was synthesized and its structure was studied in theory.the schiff base compound was synthesized by condensation of paeonol with furylfurylamine,then it was deoxidized by sodium borohydried.the energies,bound lengths,bound angles,the net charges of main atom and contributive percents of frontier orbital in the title compound have been calculated by software hyperchem 7.0 in half experience method.the yield rate of title compound was 65.6%.the result of calculation showed that the most of the bond lengths and angles in the system were in the the normal range,negative charge mainly concentrate on o1,o2,o3 and n1.the title compound behaves as quadridentate ligand.

目的合成标题化合物并对其结构进行了理论研究。方法以丹皮酚和糠胺为原料合成席夫碱,经硼氢化钠还原得标题化合物;使用hyperchem7.0程序,用半经验方法计算了化合物分子中能量分布、主要键长、键角以及主要原子的净电荷分布和前言轨道分布。结果标题化合物,收率65.6%;计算结果分析表明,分子中主要原子之间的键长、键角基本在正常的范围内;负电荷主要集中于o1,o2,o3和n1上。

Ultrasonic instruments were used to scan the normal heart transesophageally and radially.

同时用超声仪器对正常成人经食道水平和放射状扫查心脏。

Ecosystem management is the main stream of natural resource management now. The focused works of EM are collecting spatial data by remote sensing, raser surveyors and permanent sampling plots which standard for each ecosystems. Experimental management and monitoring would proceed to do adaptive management for accomplishing the management objectives of forest health, productivity and biodiversity maintaining.

生态系经营是目前自然资源经营的主,而其工作的重点在於用遥航测及卫星定位系统、射测仪等测仪器收集资,经取样、选址具有代表性的长期生态研究区或永久样区,进试验性经营、监测与适应经营调整,以达到森健康、生产提高、多样性回的生态系经营目标。

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