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Tenella YL attenuated strain were used to infect chickens perorally, then the abundant sporulated oocysts were collected by saturated saline floating measure. The size of the attenuated oocysts was about 19.8~21.2×15.5~16.6μm and the conformation of the strains were integral. 4 The prepatent period of E.

tenella YL减毒株孢子化卵囊经口服感染鸡,用饱和食盐水漂浮法能够收集得到球虫孢子化卵囊,杂质少,形态完整,减毒株卵囊的大小为19.8~21.2×15.5~16.6μm。

Methods:Fifty-six patients with metacarpal and phalangeal fractures who were reviewed in the study were divided into two groups:Group A and Group B.Thirty-two patients in Group A were treated with open reduction,Kirschner internal fixation and postoperative plaster splint external fixation.Other 24 patients in Group B were treated with open reduction,Mini-plate internal fixation,without any external fixation.

回顾分析经完整随访的56例掌指骨骨折患者的治疗和康复情况,A组:32例掌指骨骨折患者予以行切开复位交叉克氏针内固定接骨术,术后石膏夹板外固定;B组:24例掌指骨骨折患者予以行切开复位微型接骨板内固定接骨术,术后未用外固定。

"Half-water-hydrolysis"method is used to modify the preaminolysis of phenylenediamine in"step coupling polymerization". First, dehydrochlorination polycondensation between Si-OH and Si-Cl group during polycondensation reaction is employed instead of dehydration reaction between Si-OH groups before, which avoids the disconnection of Si-N bond destreyed by isolated water and improves the H-bonding template effect of the bridged-phenylenediamine.

探索用&半水水解-脱氯化氢缩聚&法对我组过去提出的&全水水解法&的预胺解法加以改进,经&半水水解&得到的含硅羟基和硅氯基的单体再以脱氯化氢代替脱水的方法减少了极性溶剂存在下游离水断裂梯撑模板的硅-氮键的可能性,有效地提高了对苯二胺梯撑中氢键的模板作用。

Comparison from the Germany isolate, phocine distemper virus type Ⅱ(PDV-2) and vaccine strain, the major difference is the long signal peptide domain while the mature protein exhibit high identity, and all of the 13 serine residues, four potential asparagine glycosylation sites and two hydrophobic regions supposed to affect the fusion function are completely identical. These data supports the view that F protein is more conservative than H protein in CDV.

三、犬瘟热病毒中国分离株囊膜糖蛋白基因免疫小鼠诱发的抗体应答经一系列步骤将CDV-YZ0101株的两囊膜糖蛋白基因定向导入真核表达载体pcDNA3.1中,DNA测序和酶切分析筛选阳性重组质粒克隆pcDNA-H和pcDNA-F,以重组质粒DNA和脂质体共转染COS-7细胞,用间接免疫荧光试验证实转染的COS-7细胞的胞浆中分别表达了CDV的H和F蛋白。

Photolettering: Method of setting display sized type photographically from film master.

照相标题字:用照相方法经菲林母片影排标题字的方法。

Results 5-FU inhibits growth of Lovo cells; it is found that within a limited dosage and period of time, there has been an obvious increase in apoptosis cells under the photomechanical scope; the analysis of Lovo cells by FCM showed that after the induction of 5-FU, the percentage of apoptosis cells has increased; and FCM also displays dosage and time effects. Electrophoresis was obviously in a stripe shape of trapeziform.

结果 5-FU能抑制 Lovo细胞生长,在一定剂量和时间范围内,通过光镜观察,见凋亡细胞明显增多,经5-FU诱导后,用FCM 分析 Lovo细胞,发现凋亡百分率增加并显示剂量和时间效应。

The fresh chicken feces were obtained from different age 18 healthy chicken, and were not detectedchlortetracycline. The mixture standard solution of CTC and OTC was added in fresh chicken feces at the concentration of 0.5, 1.0 and 4.0ug/ml respectively. The chicken feces were divided to two groups,one were put in the incubator under photophobic condition; another were under the natural illumination.Samples were collected after 0, 0.5, 1, 2, 3, 5, 7, 9, 12 and 15 days, The concentration of chicken feceswas determined by HPLC. According to the theory of degradation kinetics, we fitted the changed trendof concentrations in chicken feces under different time and studied the degradation characteristics ofchlortetracycline in chicken feces.

选取不同日龄健康鸡18只,实验期新鲜鸡粪,,经检测不含四环素类药物,然后加入金霉素标准液,使得各样品中制得的相应药品的浓度为0.5ug/mL、1.0ug/mL、4.0ug/mL,分为两个实验组,第1组放入培养箱中避光黑暗条件下;第2组置于室外自然光下,定时取样,取样时间为0、0.5、1、2、3、5、7、9、12和15d,鸡粪中药物的浓度用本实验建立的高效液相色谱法测定,根据药物降解动力学原理,对鸡粪中药物的浓度随时间的变化趋势进行曲线拟合,研究金霉素在环境鸡粪中的降解动态规律。

The platelets were labeled by FITC injected by jugular artery. Adherence of platelets to endothelial cells in pial vessel was observed by fluorescent microscope. The expression of GPⅢa protein was identified by CD61 histochemistry.

荧光显微镜下观察活体软脑膜微血管中血小板对内皮细胞的粘附,用CD61抗体经免疫组化方法观察缺血再灌注沙土鼠脑组织血管内血小板和内皮细胞上GPⅢa糖蛋白的表达。

B-glucosidase the elicitor collected from Pieris brassiae larva oral secretions , which incur indirect defense of cabbage plant to P.

用已明确的大菜粉蝶口腔分泌物诱导因子b-葡萄糖苷酶(b-glucosidase)处理小麦幼苗,经鉴定、分析,该酶没有引起小麦的间接防卫反应。

Tissue engineering is a multidisciplinary science merging with cell biology, material science, engineering and chemistry. Its basic idea is to expand a number of cells in vitro and seed them on a polysynthetic biodegradable polymer structure, and finally enable the organization of 3-dimmentional and functional tissues or organs.

组织工程技术是融合了细胞生物学、材料科学、工程学和化学的一门边缘学科,它的基本思想是用少量组织细胞经体外培养扩增后播种在可降解的生物支架材料上,再回植入体内形成有功能的组织或器官。

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