经用

Globin chain analysis proved the α chain to be abnormal The fingerprint of the abnormal α chain and the amino acid sequence of the abnormal peptide obtained indicated that the α 34 leucyl residue had been replaced by arginyl...

将异常α链经柱层析分离纯化后作指纹图，把指纹图的异常肽斑洗脱，用微量双偶合法作氨基酸顺序测定，发现异常肽斑为α34亮氨酸被精氨酸取代，由此证实此异常血红蛋白为Hb Queensα_2~(34Leu→Argβ_2。

Methods By the bioinformatics analyzing tools in bioinformatics webs site,a Ta CRISP 2 fulllength gene from the Taenia saginata asiatica fulllength cDNA plasmid libratory was identified and the coding region sequence and the characteristics of the deduced protein were analyzed.The coding region of Ta CRISP was amplified with PCR,endonuclease digestion and cloned into the prokaryotic expression vector pET30a and then expressed in E.coli BL21 with IPTG induction.

用生物信息学方法从亚洲牛带绦虫成虫全长cDNA质粒文库中识别TaCRISP的全长编码基因并预测编码蛋白质的各种结构与功能；将其编码区序列克隆到原核表达载体pET-30a上，测序鉴定重组质粒，之后进行诱导表达，表达产物经十二烷基磺酸钠-聚丙烯酰胺凝胶电泳鉴定。

The mitochondrial cytochrome oxidase subunit Ⅰ gene fragments (682 bp) were amplified and cloned from two pure alcohol preserved Lingula anatina specimens collected from Qinhuangdao, northern China.

从河北秦皇岛采集的用无水乙醇保存的鸭嘴舌形贝样品中提取了DNA，经PCR扩增后进行克隆，得到了2条长度为682bp的线粒体细胞色素C氧化酶第Ⅰ亚基基因片段。

These ferments are made of the roofs, leaves and stems of 58 handpicked kinds of plant and vegetables through exclusive the biochemical technology, with the high-reactivity, high-stability and high-safety; The second is the fruit compound ferments(the approval No.J11000510836) with the abundant vitamin, minerals, favorable viable groups, multi-amino acid groups, oligosaccha groups and glucose , is made from handpicked 103 kinds of fruits and 20 kinds of Chinese herbal medicines through microbial treatment and fermentation method; The third is grain and bean high-fiber nutritious ferments (the approval No.J11000600369) with abundant proteases, amyloclast enzyme and lipolytic enzyme, is made from 60 kinds of grains and beans through special biologic technology, and is used to lose weight, expel toxin, clear the bowels, supply nutrition and activate cells in human body.

公司精选台湾58种植物、蔬菜的根、茎、叶天然原料，以独特的生化科技手段研制而成，不仅具有高活性、稳定性和安全性的特点，而且具有耐高温、耐强酸的特点，不含任何添加剂，其活性是当今世界任何厂家生产的酵素都不可比拟的。第二宝是水果复合酵素（检验审核号是J11000510836）。公司精选台湾103种水果，通过微生物科技处理，经半年多时间发酵酿制而成，含有丰富的维生素、矿物质、有益活菌群、氨基酸、果寡糖、葡萄糖等。第三宝是谷豆高纤营养酵素（检验审核号是J11000600369）。公司精选60种谷、豆及种子，用生物科技手段，经过特殊研制而成，含有丰富的蛋白质分解酵素、淀粉分解酵素及脂肪分解酵素。

T included a land tax which the prime minister .loyd george ,had put it on purpose, knowing that the majority of land-owning lords would resist it.it was a clever trap,and they fell straight into it .for centuries, custom had allowed them to refuse an ordinary bill;but this time they were refusing a budget because it was against their own interests.it was time that their power was reduced.the next year Edward VII died,and a new election showed that the people supported the government,Edward's son,george v,had to make the lords change their minds.he threatened to make now liberal lords,just as william iv had done to pass lord grey's reform bill.the country was relieved to see that the royal power could still be used to support its will,and the position of the crown was strengthened.

这是一个绝妙的陷阱，他们跳了进去。几百年来，习惯上他们可以拒绝通过普通的法案，但这一次他们拒绝的是政府预算，因为经有悖于他们切身利益。这正是削弱他们势力的时机。次年，爱德华七世去世，新一轮选举表明人民支持政府。爱德华的儿子乔治五世不得不说服上议院改变主意，他威胁上议院，他要加封自由党新贵族，正如威廉四世为通过《格雷议会改革法案》时所采取的措施那样。全国人民欣慰地看到皇室权势还能用来支持他们的意愿，皇室的地位得到了加强。

The colour of most detected bands became light in the five media with the order of mannosan, mannose, xylan, glucose and pectin.(4)The pectinase in the supernatant was purified by centrifuge , acetone precipitation and sephadex G-75 cloumngel filtration. The specific activity was increased 9.8-fold with an activity recovery of 32.98%.The molecular weight of pectinase was 42.2KD estimated by SDS-PAGE.

确立了以葡萄糖培养基6h的发酵液为提取果胶酶的最初取样点，发酵液经离心，丙酮沉淀，Sephadex G-75葡聚糖凝胶柱层析后获得了凝胶电泳均一的样品，比活力较原酶液提高了9.8倍，回收率达到了32.98%，用SDS-PAGE凝胶电泳测的纯化后的果胶酶分子量为42.2KD。

Such as thin lightweight precast concrete blocks, hollow clay blocks and timber studding covered with either plasterboard, fibreboard, matchboarding, plywood, chipboard, resin bonded blockboard or other form of cladding.

如用轻薄混凝土砌块、空心砖、细木条再覆以壁纸板、纤维板、假型板、胶合板、经纤化处理后的废纸板、松脂粘合板或各种类型的复合材料板等。

Medical heavy magnesium carbonate was made from soda (NaCO3) and magnesium chloride (Mgcl2*6H2O) by the metathetical reaction followed by pyrolysis,separation and desiccation.

方法用纯碱和盐卤作为原料，经复分解反应后再热解、分离、干燥制得医用重质碳酸镁。

The crudeglycerol from biodiesel production could be directly converted to 1,3-PD without any priorpurification. In flask cultures, the 1,3-PD yields of 0.62 and 0.44mol/mol on crude glycerolfrom lipase-and alkali-catalyzed methanolysis of soybean oil were lower than that of0.68mol/mol on pure glycerol. In fed-batch fermentations, the 1,3-PD yields of 0.47 and0.46mol/mol on crude glycerol were comparable to that of 0.49mol/mol on pure glycerol.

生物柴油的副产物甘油不经纯化可以直接用来发酵生产1，3-丙二醇，在摇瓶培养中，由酶法和碱法得到的甘油获得的1，3-丙二醇的转化率分别为0.62mol／mol和0.44mol／mol，而纯甘油得到的转化率为0.68mol／mol；在批式流加发酵中，由酶法与碱法得到的甘油和纯甘油得到的1，3-丙二醇的转化率相差不大，分别为0.47mol／mol、0.46mol／mol和0.49mol／mol，纯甘油得到的生产强度最高，为2.0g／，两种粗甘油得到的生产强度都为1.7g／。

He impedance change of pulmonary circulation was detected With the esophageal electrodes put in the esophagus below the left atrium of rabbits and the surface electrodes of the thorax in the fifth intercostal space at the right midaxillary line.

将食道电极置于家兔食道下段左房水平以下，将体表同心圆电极置于右腋中线第五肋A间，用食道电极结合体表电极检测肺部血管阻抗变化，此法简称经食道肺血流图。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新