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Hen THP-1 macrophages were induced to the foam cells by ox-LDL. Treatment of THP-1 macrophage derived foam cells with apoA-Ⅰ, forskolin (FRK, an adenyl cyclase activator), and SQ-22536 for long periods of time (24 h). In addition, THP-1 macrophage derived foam cells were treated with increasing amounts of FRK (0, 10, 20, 40 and 80 μmol/L) and treated with FRK for increasing time(0, 6, 12 and 24 h).

HP-1巨噬细胞源性泡沫细胞经各种因素处理后,采用油红&O&染色,观察细胞内的脂滴,运用液体闪烁计数器检测细胞内胆固醇流出,高效液相色谱分析细胞内总胆固醇、游离胆固醇和胆固醇酯含量,用逆转录-聚合酶链反应和蛋白质印迹分析法分别检测ABCA1 mRNA与ABCA1蛋白质的水平。

The fusion protein was purified through Glutathione-Sepharose 4B affinitive chromatography and GST part was cut off with thrombin.

然后经Glutathione-Sepharose 4B亲和纯化后,用凝血酶切除GST部分。

The skillful application of various materials and the updating of the treatment approaches become a new form of expression as well as one factor enlightening the artist: the painting pigment for Zangzuxiangba adopts the natural ores and plant raw materials which is produced on snow-covered plateau and characterized by pure color, high quality, good light fastness, strong weather resisting property, fast coloring and gorgeous but not ordinary hue; the paper for Zangzuxiangba is Tibetan paper taking radix stellerae chamaejasmes and agilawood produced on snow-covered plateau as raw materials and manually manufactured by scores of procedures, thus having such properties as corrosion protection, mothproof property and dampproof property.

同时,藏族祥巴在颜料和用纸应用上也不断探索和创新,其对各种材料的巧妙应用和处理手法的更新,不仅成为一种新的表现形式,也成为启迪艺术家创作灵感的因素之一:藏族祥巴的绘画颜料采用产于雪域高原的天然矿、植物原料,色泽纯正、品质优异,耐光极佳,耐候性极强,具有色彩历久不褪、色相艳而不俗的特点;藏族祥巴的用纸是用产生高原雪域的瑞香狼毒、沉香等为主要原料,经数十道工序手工制作而成的藏纸,具有防腐、防蛀、防潮的特性。

Aglaonema commutatum White Rajah was used as the main test material to study the technology of hydroponic culture with different concentrations of indole-3-acetic acid treatrnent to induce aquatic root system and choose the best concentration of IAA.

以天南星科植物白雪公主为试材,采用水培法经不同浓度的吲哚乙酸处理,进行诱导水生根系试验,然后用诱导的水生根系材料进行营养液培养。

The following question can be used to distinguish real-time systems from the rest:"is alate answer as bad, or even worse, than a wrong answer?"

以下的问题可经被用来区分实时系统与其他的系统:&是迟到的回答坏,还是一个错误的回答坏?&

TiO2 powder was prepared from TiCl4 with addition of aqueous ammonia by changing Ti4 hydrogel into its corresponding alcogel followed by ethanol-thermal ,dryness and calcination.

用四氯化钛为原料,经氨水沉淀、水洗、无水乙醇洗涤得到Ti4醇凝胶,对Ti4醇凝胶进行乙醇热处理、干燥、焙烧制得了TiO2粉体。

He taught himself Hebrew in readiness for his aliyah , or formal return to Israel.

但是他从来没有忘记,他自学用希伯来语读经或正式访问以色列。

METHODS: From January 2000 to May 2002, 52 cases with various types of bone defect were treated with tissue engineered bone, which was constructed in vitro by allogeneous osteoblasts from periosteum 1 x 10(6/ml with bio-derived bone scaffold following 3 to 7 days co-culture. Among them, there were 7 cases of bone cyst, 22 cases of non-union or malunion of old fracture, 15 cases of fresh comminuted fracture of bone defect, 4 cases of spinal fracture and posterior route spinal fusion, 3 cases of bone implant of alveolar bone, 1 case of fusion of tarsotarsal joint.

2000年1月~ 2002年1月,用同种异体骨膜来源的成骨细胞 1×106 / ml与生物衍生骨支架材料构建的组织工程骨,经体外培养 3~ 7天后,植入修复52例各种类型的骨缺损,其中骨囊性病变7例,陈旧性骨折不愈合或畸形愈合 2 2例,新鲜粉碎性骨折伴骨缺损 15例,脊柱骨折后路植骨融合术4例,牙槽骨植骨 3例,足跗中关节植骨融合 1例。

Methods: 60 SD rats were divided into 4 groups randomly, the nomal group, the model group, QZHJ group, and the hydrocortisone group. Rats were sacrificed in bdtch on the 3rd, 7th and 21st day after treatment, then the concentration of interleukin-8(IL-8) in bronchoalveolar lavage fluid and the content of transforming growth factor-β1 (TGF-β1) of lung tissue were deected, while the severity of alveolitis and fibrosis were measured.

将60只健康SD大鼠随机分为正常组、模型组、芪术合剂组和强的松组,经气管滴入博莱霉素复制肺纤维化大鼠模型,并分组予以治疗,于治疗后第3、7、21日分批处死大鼠,每组每次6只;用免疫组化法观察肺组织转化生长因子-β1的表达,并测定支气管肺泡灌洗液中中性粒细胞和白介素-8的水平。

Purpose With immunohistochemical methods under the light and eletronic microscopic level, together with the computer analytic techniques , the expression changes of the synaptophysin in the hippocampus of the rats that have obtained the abilities of spatial discriminative learning and memory established by water maze during the different periods will be studied, after they stopped training. Probing into the relationship between the amenia of the spatial discriminative learning and memory with the expression changes of the synaptophys in.

目的 用免疫组化的光、电镜方法结合图像分析技术,检测经水迷宫训练获得空间辨别性学习记忆功能后的大鼠在停止训练的不同时间段其海马结构内突触素表达的变化。

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年美国个人破产法的一个改动使得破产登记急速下降,而后引起了信用卡大规模的亏损。

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弗四23 而在你们心思的灵里得以更新

Lao Qiu is the Chairman of China Qiuyang Translation Group and the head master of the Confucius School. He has committed himself to the research and promotion of the classics of China.

老秋先生为中国秋阳翻译集团的董事长和孔子商学院的院长,致力于国学的研究和推广。