经用

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

Industrial residue is combined with coke dust to produce granular materials that is transferred to liquation furnace for high - temperature processing after automatic computation and proportioning , and the melt is processed to be fiber by fiber machine , then after containing dust , floatation deslagging, shot separation and modification processing , whitening and dehydration , which the fiber is pressed to inorganic fiber for paper making in vacuum condition and the product is weighed,packaged and put in storage automatically .

工业废渣配焦炭粉造粒料，经自动计量配比后送熔化炉高温处理，熔化液经成纤机加工成纤维，再经集尘，气浮除渣，渣球分离及改性处理后，漂白脱水，真空压榨成造纸用无机纤维产品，自动称量包装入库。

Steering by automatic pilot and by magnetic compass in stead of automatic pilot, Measure the exactness of magnetic compass by qualified calibrator and provide compass adjustment card.

自动驾驶仪操舵和用磁罗经代替自动驾驶仪，通过合格的校准器来测定罗经的正确功能并提供罗经校正卡。

There were three major findings.(1) On the basis of the assessment of overall model fit and parameter estimates, the hypothesized second-order confirmatory factor analysis model represented an adequate fit to the sample data.(2) According to the cross-validation analysis, the second-order factorial validity of this scale was very stable.(3) This hypothesized model was invariance across these five important demographical variables, including school, gender, grade, right/left-handedness, and past experience in unintentional injury, via the testing of invariance in the multiple groups.

结果有三项主要的发现：第一、经整体暨个别的残差分析、整体模式之适配与其取代指标，以及估计参数之统计考验等结果，均指出假设此量表为二阶因素分析测量模式是相当恰当的；第二、经交互确认效度分析结果，显示此量表之二阶潜在因素结构是非常稳定；第三、经多组别不变量统计考验结果，指出此量表之二阶潜在因素结构，对於学校别、性别、年级、用手习惯，以及过去伤害经验这些社会人口变项，大致上具有因素个数、因素负荷量、因素变异数，以及测量误差变异数等不变量的特性。

Air Sea: Located in the lower abdomen before the middle line yfzhg05, 1.5-inch lower navel, with help Qi Yang, fixed upon the role of menstruation can be used to treat men, enuresis, impotence, nocturnal emission, Hua Jing, women's menopause, uterine bleeding, vaginal discharge , and neurasthenia.

气海：位于下腹部前正中线上，脐中下1.5寸，具有益气助阳、调经固经的作用，可以用来治疗男子遗尿、阳痿、遗精、滑精，女子闭经、崩漏、带下，及神经衰弱等。

The stepping-motor-driven warp let-off unit is applied to 1515-PJR flexible rapier loom. The warp tension is measured by using IBM-PC and CRAS software package. A new measuring index of warp tension for comparing the performance of warp let-off motion is given. The statistical analysis on the warp tension in the weaving process from full warp beam to empty is conducted. Also, the spectrum of the dynamic warp tension is analyzed, and the influences of the warp let-off motion' timing on warp tension are discussed.

本文研制的步进电机驱动送经装置在1515-PJR挠性剑杆织机上进行了实际织造运行，用IBM-PC微机和CRAS软件包对经纱张力进行了现场测试，提出了一项新的衡量送经装置性能好坏的经纱张力测量指标，对从满织轴到空轴织造过程中的经纱张力水平进行了统计分析，对经纱动态张力信号进行了频谱分析，并研究了送经运动的时间安排对经纱张力的影响。

And this paper also shows the structure and parameters of the optical system. The Xenon light of super high voltage is used as interferential source. The position of it is on the focus of the reflector of ellipsoid surface. After reflecting, its lights form a collective point . This point and objective focus of collimation lens are superposition. Though the collimation lens, the beam of light become parallel lights. Then though the Coude Reflector and radiation system of special prism, the lights become a beam of light with 3 ° emanative angle.

该光学系统选用超高压氙灯作光源，使其位于椭球面反射镜的一个焦点上，发出的光线经椭球面反射后所形成的汇聚点与准直负透镜组的物方焦点重合，光束经准直系统后变为平行光束，此平行光束再经库德反射镜和特殊发散棱镜系统，形成具有3°发散角的发散光束，用来干扰敌方设备。

Those biodegradable materials finally form into CO2 and H2O via culture of tricarboxylic acid cycle, which can be excreted from lung, kidney, and skin. Hydroxyacetic acid is a metabolic product of amino acids. Therefore, velocity of bioldegradable materials can be controlled in vivo. The recent contraception focuses on discover a sustained release system of biodegradable materials.

生物可降解材料包括聚乳酸、聚ε-己内酯、乳酸-羟基乙酸共聚物等，这些材料有很好的生物相容性，植入体内后可经生物降解成乳酸单体，经三羧酸循环后最终成为CO2和H2O，经肺、肾、皮肤排泄，羟基乙酸也是机体氨基酸代谢产物，而且生物可将解材料在体内降解速度是可控的，用生物降解聚合物作为药物载体的缓释避孕系统是目前材料学以及避孕节育研究的一大重点。

Results Out of the4527paˉtients treated by ESWL,stones were excreted completely from4512patients within2weeks;12patients who had comˉplication of the urethra stone street were cured by transcutaneous nephrocentesis,nephrostomy and ureterolithotomy through uretercystoscope;stones weretaken out by operation from the other3patients,from whom the excretion of stones was not possible.

目的 探讨ESWL治疗泌尿系结石的效果。方法回顾性分析经ESWL治疗的4527例泌尿系结石病人。结果经ESWL治疗的4527例泌尿系结石病人中，4512例在2周内排尽结石，12例并发输尿管&石街&，加用经皮肾穿刺造瘘和输尿管镜取石治疗，3例中转手术治疗。

METHODS:The gene of human ET1 was synthesized according to the preferential codons of E. coli, cloned into the EcoRI and SalI sites of vector pThioHisA. The recombinant plasmid pThioHisA-ET1 was constructed , sequenced and transformed into E.coli TOP10. Induced and expressed fusion protein were identified and analysed by 12% SDS-PAGE and densitometry analyses. After the elution, denature and renature, the fusion protein Thioredoxin-ET1 was obtained by ProBondTM chromatogragraphy. The purity of Thioredoxin-ET1 was detected by HPLC. Inoculate Thioredoxin-ET1 once per mouse every 2 weeks in 25, 50 and 100μg separately on 3 groups for 4 times. 10 days after last inoculation, we obtained venipuncture blood.

根据人ET1的多肽序列合成ET1基因，将其插入到pThioHisA的EcoRI和 SalI位点，重组质粒pThioHisA-ET1进行酶切鉴定及序列测定验证后转化TOP10，IPTG诱导的重组菌经SDS-PAGE检测融合蛋白Thioredoxin-ET1的表达量；表达的融合蛋白用ProBond亲合层析纯化并经HPLC鉴测其纯度；每只小鼠按25、50、100ug/次剂量的Thioredoxin-ET1每两周免疫一次，共4次，最后一次免疫10d后制备抗血清，经Western blot和ELISA检测证明Thioredoxin-ET1融合蛋白具有ET1免疫反应原性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。