经用

The best optimum conditions of the Agrobacterium mediated genetic transfornation were that leaves explants were immersed in the diluter 10 times Agrobacterium bacilli at OD600 of 0.6 for 8 minutes after being pre-cultured for 2 days,and then co-cultivated for 3 days.Then these explants were transferred to selected and rooting medium which contained 50 mg/L Kanamycin and 500 mg/L Carbenicillin.According to the transformation system, 54 transgenic plants were initially screened,then after PCR analysis and RT-PCR analysis 25 transgenic plants were obtained.

根癌农杆菌介导的芪合酶基因遗传转化的最佳条件为：将预培养2 d的烟草外植体，用稀释10倍的GV3101菌株菌液（OD600=0.6）浸染8 min后，共培养3 d，然后转入含卡那霉素50 mg/L和羧苄青霉素500 mg/L的筛选分化培养基上诱导分化，待抗性芽长到2～3 cm后，转入含Km 50 mg/L和Cb 500 mg/L的筛选生根培养基上进行筛选，在以上最佳遗传转化条件下，初步筛选到54株转化体，经PCR和RT-PCR检测获得25株转化烟草植株。

Materials and Methods: 12 patients with confirmed liver tumor (4 hepatocytic carcinoma, 8 hepatic metastases) were treated with a Diomed semiconductive laser device.

材料和方法：经病理证实肝肿瘤12例（原发肝癌4例，肝转移癌8例），用达美德半导体激光治疗仪行激光治疗。

SW620/pRETRO-On-TRAIL cells become round and small,dioptre of ellsbroken cells increase.

用相差显微镜观察细胞形态发现，SW620/pRETRO-On-TRAIL细胞经四环素诱导后，变圆变小，贴壁性差，折光性强，细胞碎片增多。

Ethernet data stream with HTML format and DVB-C data stream with MPEG2 format is inputted in form of ASI and SPI, and send it into FIFO via 74F675A. 5416 packs and encrypts and transfers to DVB-C and Ethernet by time diplex mode. Thereby, data bus access is realized.

HTML格式以太网数据流和MPEG2格式DVB-C数据流以ASI和SPI形式输入，经74F675A串并转换进入FIFO.5416进行打包和加密并以时分复用方式传输DVB-C和以太网数据，实现数据总线访问。

Article 8 The mission may for official purposes communicate freely with the Government and the other missions and consulates of the sending State In so doing, it may employ all appropriate means, including diplomatic couriers, diplomatic bag, and messages in code or cipher

第八条 使馆为公务目的可以与派遣国政府以及派遣国其他使馆和领事馆自由通讯。通讯可以采用一切适当方法，包括外交信使、外交邮袋和明码、密码电信在内第九条使馆设置和使用供通讯用的无线电收发信机，必须经中国政府同意。使馆运进上述设备，按中国政府的有关规定办理

Milk volumes are measured by means of a calibrated dipstick or, more often nowadays, by use of an automatic flow meter on the collection vehicle .

牛奶的体积可以用经校准的量尺测量，现在，更多是利用收奶运输装置上的一个自动流动计量器。

By Code extraction method is to investigate the edge of each image pixel in a neighborhood Gray-scale changes in the region, using the edge of the neighboring first-order or second-order directional derivative Changes of a few simple ways to detect the edge.

经 典的边缘提取方法是考察图像的每个像素在某个邻域内灰度的变化，利用边缘邻近一阶或二阶方向导数变化规律，用简单的方法检测边缘。

The iris radius of 60 (93 eye) patients with early pupillary block primary angle closure glaucoma was measured with this method, it showed:(1) there was a statistically significant difference in iris radius between the patients with early stage primary pupillary block angle closure glaucoma and the normal subjucts (53 cases 106 eye) with the age matched group (P.01),(2) it is proved with the stepwise discriminatory analysis that the iris radius-the most significant bio-anatomic parameter of the anterior segment is usefull to diagnose early primary pupillary block angle closure glaucoma by stepwise discriminatory analysis.

本研究用此测量法测量了60例93只眼早期原发性瞳孔阻滞性闭角型青光眼的虹膜曲率半径，发现：（1）早期原发性瞳孔阻滞性闭角型青光眼患者的虹膜曲率半径小于同年龄正常人（53例106眼）的虹膜曲率半径，其差异具有显著性统计学意义（P.01）；（2）经多因素逐步判别分析证明本研究所提出的活体眼前节解剖学测量参数——虹膜曲率半径有助于原发性瞳孔阻滞性闭角型青光眼早期诊断。

Its steps include these:(1) purification of WPA;(2) neutralization reaction between purified WPA and sodium carbonate for the salt mixture of disodium hydrogen phosphate and monometallic sodium orthophosphate;(3) adjustment of the mixture's neutralizing degree with additive acid or alkali;(4) quick separation of crystal with additive organic solvent under continue stir;(5) polymerization of crystal under the high temperature for the high quality product of STPP.

其步骤为：(1)湿法磷酸净化；(2)净化后的磷酸与碳酸钠中和反应生成磷酸一氢钠和磷酸二氢钠混合盐溶液；(3)用酸、碱调节确定该混合盐溶液的中和度；(4)加入有机溶剂，通过不断地搅拌使晶体快速析出；(5)晶体经高温聚合，生成高品质的三聚磷酸钠。

Methods All 6 cases had endophthalmos and blepharophimosis. Four cases also complained of diplopia. The horizontal and coronal CT of orbit showed the lamina papyracea fracture clearly.The degree and range of the lamina papyracea displacement was measured on CT films.The reduction under nasal endoscope was performed with local and topical anesthesia. We selected the Meeserklinger＇s route of entry.The uncinate process,ethmoidal bulla,and ethmoidal cells were removed successively.Then,with the help of a tonsil dissector, the lamina papyracea was pushed outward and replaced and was supported with iodoform gauze.After 2 to 3 weeks,the gauze was removed.

对6例伴有眼球凹陷、睑裂变小及复视等症状、眶部CT扫描均显示纸板击出性骨折的患者，在确定骨折位移程度及范围后，在局部麻醉＋鼻粘膜表面麻醉下行鼻内窥镜经筛窦纸板骨折复位术，手术采用常规Meeserklinger入路，小心咬除骨折区筛房，显露骨折区纸板，用钝性剥离子，由前向后将内移的纸板外推复位，局部碘仿纱条填塞支撑，2～3周去除填塞纱条。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。