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The recombinant xVEGF and mVEGF were obtained and purified by Ni-NTA affinity chromatography under denature conditions and enterokinase specific digestion, respectively.

表达产物经Ni-NTA亲合层析及肠激酶特异性酶切进行分离纯化,最后用SDS-PAGE和蛋白免疫印迹法进行鉴定。

Methylation production were hydrolyzed, deoxidize, then analyzed by GC-MS.

甲基化产物经酸水解、还原、乙酰化后用GC-MS联机分析。

It's fixed by swing depressor fixtume structure thedespower can be assured by checking computations.

在前述定位基准面选择的前提下,用摆动式压板杠杆夹紧结构,夹紧力经验算可以保证。

It's fixed by swing depressor fixtume structure the despower can be assured by checking computations.

在前述定位基准面选择的前提下,用摆动式压板杠杆夹紧结构,夹紧力经验算可以保证。

On the base of the single-diameter laser-scanned measuring technology, a double direction laser-scanned measuring system is presented. Which combinate the laser-scanned measuring technology with special optical system. In the system, the collimation semiconductor laser-scanned beam scanning two perpendiculars direct of one plane of the measured workpiece at the same time is made. The beams with the dimension information of two perpendiculars direct are processed by the scanning receive system, the high-speed photoelectric transition and electronic data process. Two measured results of the diametric directs and ellipse tolerance,etc, parameter, of the turning workpiece on the same plane are obtained by non-contact automatic measurement. The problem of non-contact measurement of two perpendiculars direct of one plane of turning workpiece is settled. It processes many peculiarities, such as high speed, high accuracy and non-contact automatic measurement, etc.

在单向激光扫描检测技术的基础之上,提出了一种双向激光扫描检测系统,其采用激光扫描检测技术与特殊光学系统相结合,用准直半导体激光扫描光束对被测工件径向某一截面的两个相互垂直方向同时扫描,经扫描接收光学系统、高速光电变换、电子学系统和微机数据处理系统,对将携带有垂直方向被测量信息的光束进行处理,实现了回转体工件同一截面两个垂直方向的径向尺寸和椭圆度等参数的非接触自动测量,解决了同时非接触测量回转体零件同一截面两个径向尺寸的难题,它具有高速,高精度和非接触自动测量等特点。

In the system , the collimation semiconductor laser - scanned beam scanning two perpendiculars direct of one plane of the measured workpiece at the same time is made . the beams with the dimension information of two perpendiculars direct are processed by the scanning receive system , the high - speed photoelectric transition and electronic data process . two measured results of the diametric directs and ellipse tolerance , etc , parameter , of the turning workpiece on the same plane are obtained by non - contact automatic measurement

在单向激光扫描检测技术的基础之上,提出了一种双向激光扫描检测系统,其采用激光扫描检测技术与特殊光学系统相结合,用准直半导体激光扫描光束对被测工件径向某一截面的两个相互垂直方向同时扫描,经扫描接收光学系统、高速光电变换、电子学系统和微机数据处理系统,对将携带有垂直方向被测量信息的光束进行处理,实现了回转体工件同一截面两个垂直方向的径向尺寸和椭圆度等参数的非接触自动测量,解决了同时非接触测量回转体零件同一截面两个径向尺寸的难题,它具有高速,高精度和非接触自动测量等特点。

The cationic diamide softener can be emulsified by emulsifying agent, and the emulsion of cationic diamide softene can be used as softening agent in Papermaking. The results showed that the emulsion of the cationic diamide softener is stable and homogeneous when the dosage of emulsifying agent cetyltrimethylammonium chloride is 10% in the emulsifying process.

摘 要:以硬脂酸和二乙烯三胺经酰化反应制得二硬脂酰化物,用环氧氯丙烷阳离子化后,再加入乳化剂乳化,可制得带有反应性基团环氧基的新型双酰胺阳离子乳液型纸张柔软剂。

The obtained Dichondra Repens forst polysaccharides were determined by spectrophotometry at wavelength 490nm with phenolsulfuric acid as a color reagent.

多糖经苯酚-硫酸显色后在490hm处用紫外-可见分光光度计进行比色测定。

After the HRP was treated with different strength of electric field,the effect of electric field on the secondary structure of HRP was studied by circular dichroism.

辣根过氧化物酶经不同强度的电场处理,用圆二色光谱研究了电场对辣根过氧化物酶二级结构的影响。

Thirdly, in order to improve the learning efficiency and stability of feedforward neural network, a fast learning algorithm for neural networks based on CGM-OC approach is presented. Compared with other learning methods such as BP method, Broyden Flecher Goldfarl Shanno method and Powell method in which the derivative is replaced by the difference quotient etc, simulation results show that the proposed method is an efficient and fast one.

第三,为了提高前馈神经网络权值的学习效率及稳定性,提出一种基于正交校正共轭梯度优化方法的神经网络学习算法,通过与其它学习算法(如:BP算法、变尺度法、用差商近似代替导数的Powell法等)的比较,经仿真试验表明,本算法是一种高效、快速的学习算法。

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。