经用

I open mailbox monthly, a pill of bill in it. Tap water, electricity, Gas, telephone and broadbone internet, moble phone,and so on. These fees usually was well controlled, not be a trouble matter. But the housing loan and credit card loan is the most in my life.Every month I controlled the credit card spending. I am furtunate that I can endure the figures of credit card bill. I paid off the bill, no debts.

昨天做了一个很奇怪的梦，梦见我又回到尚飞，辛苦的做了一天，周志麟跑过来问我情况怎么样，我说我明天不做了，回家了，这小子不满意用拳头打我，我也不是好惹的，一顿暴打，这小子被我打的口吐白沫，倒地抽搐，没想到表面上貌似很强悍的Mattew其实不经打，三拳两拳就趴下了。

Two approaches are employed to prepare thiocyanuric acid derivatized gold nanoparticles, including the direct reduction of gold ions by potassium bitartrate and NaBH〓 and then through ligand exchange reaction.

用两种不同方法合成了三聚硫氰酸衍生的金纳米粒子，包括由酒石酸氢钾直接还原金离子及NaBH〓还原金离子后经配体交换反应。

Methods Guinea pigs were, to begin with, given MBP through the nasal route to induce the development of peripheral immune tolerance, and then immunized with specific antigen MBP plus complete Freund's adjuvant. The clinical symptoms of guinea pigs were investigated, and the erythrocyte rosette test, and lymphocyte blastogenesis test, the MBP-specific skin delayed-type hypersensitivity response and the levels of interlukin-4 (IL-4) and interferon-γ were also detected, and the pathological changes were examined as well.

先使豚鼠经鼻粘膜摄入MBP，诱导建立外周免疫耐受，然后用MBP加完全弗氏佐剂免疫豚鼠，观察动物的临床症状、E花环形成试验、淋巴细胞转化试验、迟发型超敏反应、细胞因子水平及病理改变。

We also confirmed in this paper that it is injected somatic cells but not broken cytoplasm formed multi blastomere through mitosis in the reconstructed embryos.

在本实验中还通过用DNA染料（Hoechst-33342）对核移植体进行染色的方法，对融合后成纤维细胞在核移植体内不同时间的运行及发育模式进行了观察，确认了是成纤维细胞经有丝分裂形成多个卵裂球而不是由于细胞质碎裂形成的两个或多个碎块。

Objective:To analyse the McAb anti blood group A substance secreted by 50A hybridoma at molecular level and then lay the foundation for preparation of the reagent used for blood grouping of new generations genetic engineering antibody.

从杂交瘤细胞提取总RNA，经RT－PCR扩增、克隆抗体的轻、重链可变区基因，用PCR及双脱氧核苷酸链终止法分析其核苷酸序列，采用PCR技术重叠延伸拼接法构建50A单抗单链抗体基因。

From there, use of boxcar cow to Chicago, after slaughtering, cutting and freeze and then transported to the East.

从那里，用棚车把牛运至芝加哥，经屠宰、切割和冷冻后再运往东部。

For excluding poorly preserved shells, a shell alteration index is suggested to estimate the state of preservation of brachiopod shells through examination under cathodoluminescence and scanning electron microscopes. This study shows brachiopod taxa e.

基于壳体保存程度的详细分析，以阴极发光程度、壳体显微结构为参数建立了壳体成岩作用改造程度的量化模式，经化石壳体氧、碳同位素值的验证，这一模式基本可以用来对不同保存环境下的样品进行筛选。

The replica material used in this study is Polydimethylsiloxane. The enlargement of replica compared to original size is 2.34%. Since PDMS is transparent, a layer of metal should be sputtered on its surface to measure optical characteristic, however the accuracy will be affected by the metal coated. The result assumes that Sternocera aquisignata, Chrysochroa rajah and Catoxantha opulenta may have one-dimensional photonic crystal structure. The fabrication of quasi-structure with elytra from Buprestid can promote the applications in the field of photonic structure and optical components.

经PDMS翻印的结果，最小仅增加原始尺寸的2.34%，翻印结果良好，但因翻印结果需镀上一层材料，使得镀上的材料严重影响结构的反射率，造成结构不同反射率也类似的情形，将结构刻画在不需镀金的材料上，将可防止镀上材料影响反射率的问题，并将所制成之结构用於防伪、光子结构研究和光学元件上。

Ethyl 4-[(1-hydroxy-1-methyl)ethyl]-2-propyl-1H-imidazole-5-carboxylate,the key intermediate of Olmesartan ,was synthesized from butanoic acid and o-phenylenediamine via condensation under microwave-irradiation,oxidation,esterification and the Gringard reaction.

用正丁酸与邻苯二胺为原料，在微波照射下缩合成2-丙基苯并咪唑，再氧化开环制得2-丙基咪唑二羧酸，再经酯化、Gringard反应制得抗高血压药物奥美沙坦的关键中间体2-丙基-4-[(1-羟基-1-甲基)乙基]-1H-咪唑-5-羧酸乙酯，总收率32.2%。

The sample was extracted with carbon bisulfide, and the extraction was injected into the GC system for analysis after dehydration by sodium sulfate anhydrite.

水样用二硫化碳萃取，萃取液经无水硫酸钠脱水后在适宜的色谱条件下进样分析，5种氯苯类化合物的回收率为92.3%~102.5%，精密度为

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。