经用

At the end of 12 and 18 weeks, rats were killed, taken the muscle of posterior limb, after separating the gastroenemius、soleus、plantaris, to prepare the frozen section of the each kind of muscle respectively to be confirmed to form the myopathy model by the histochemical stain.(3) RT-PCR、Western blot and immunohisto-chemistry stain were respectively used to detect the expression of NF-κB、MMP-2 and MMP-9 during different period.

2实验组用酒精给大鼠灌胃，对照组用含与酒精体积相等的生理盐水灌胃，两组均喂饲改良的全营养高脂饲料，于12周及18周后，取后肢肌肉，在分离出腓肠肌、比目鱼肌、跖肌后，将各类肌肉均分别地制备成冰冻切片和石蜡切片，经组织化学染色确认成模。

The wall-broken Cole bee pollen lOOg is extracted by 1:10 of anhydrous ethanol , continuously stirred in darkness for two hours. And then by means of centrifuge, cleaning, vacuum concentration, adjusting pH value, anion exchange resin chromatography, propanone extraction,being blown to dry and freeze-dried, we use HPLC to detect the content of the GA derivative of the freeze-dried powder.

将破壁油菜蜂花粉100g，在避光条件下用无水乙醇(料液比1：10)连续搅拌浸提2小时，浸提液经离心、洗涤、真空浓缩、pH值调节、阴阳离子树脂交换、乙酸乙酯萃取、吹干再冻干后用HPLC法分析，得冻干样品中赤霉素衍生物的含量为51.29％。

In this paper, the synthesis of polyamide acid resin, with 4, 4′-diam-inodiphenyl ether and pyromellitic dianhyride as monomers and dimethylacetamide as solvent, is reported; the preparation of polyimide fiber from PAA resin by dry- spinning method is described; and the effects of various factors of operating conditions upon the mechanical properties of the fiber is discussed.

本文报导了用4，4′—二氨基二苯醚与苯均四酸酐作单体，在N，N′—二甲基乙酰胺溶剂中合成聚酰胺酸树脂，以及用该树脂经干纺法制备纤维和制备过程中各项参数对纤维力学性能的影响。

Then recrystallize the thick product with the petroleum ether, the end product obtains its melting point after infrared lamp drying with the melting point detector and compares with the pure Diphenyl carbinol.

粗产物用石油醚重结晶，终产品经红外灯烘干后用熔点测定仪得到其熔点，并与纯品二苯甲醇比较。

With ultrasonic wave and lysozyme, the recombination N protein is split from bacteria. We dissolve it in guanidine hydrochloride, purify it with Ni〓 affinity chromatography column and renature it in vitro. By testing the antigen of the purified N protein, it shows that its activity is very high.

建立了用超声波和溶菌酶裂解菌体，用盐酸胍溶解，Ni〓亲合层析柱纯化及体外复性等方法，经检测证明纯化的VSV重组核蛋白抗原具有较高的活性。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，将溶酶体的靶向信号肽KFERQ连接在RTA的羧基端；将构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受态大肠杆菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，將溶酶体的靶向信號肽KFERQ连接在RTA的羧基端；將构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受態大肠桿菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

A complete bovine 18ku-bFGF gene was cloned by nested-PCR and subcloned into expression vector pET-28a.The recombinant plasmid pET-28a-bFGF was transformed into Escherichia coli BL21.At 25℃,recombinant protein was induced by 0.5mmol/L IPTG for 5 hours.Supernatant of cell lysate was purified using Ni-NTA method and the products was submitted to detect the bioactivity by Western-blotting,which indicated that the fusion protein contained recombinant bovine bFGF.Results showed that recombinant bovine bFGF was produced and solubly existed in the supernatant.NIH-3T3 fibroblasts were used to detect the bioactivity of the fusion protein.

采用巢式PCR方法克隆了牛18ku-bFGF基因完整的编码序列，并构建了原核表达载体pET-28a-bFGF，将其转化大肠杆菌BL21，在25℃低温条件下，用0.5mmol/L IPTG诱导表达5h，用Ni-NTA亲和纯化细胞裂解上清液，经Western-blotting检测，结果显示，在特定的诱导条件下，重组牛bFGF基因在大肠杆菌中获得了表达，并且主要以可溶性状态存在于细胞中。

Methods: By phenol-vitriolic coloring, determining its content by spectrophotography and analyzing its composition by PC method.

经苯酚－硫酸显色，用分光光度法测定多糖含量；用Pc法检识多糖成分。

Technology of laser hardening surface of diesel locomotive camshaft using austenite-bainite nodular-iron instead of 20Cr has been systematically elaborated on,which includes a series of measurements such as mechanism of choosing austenite-bainite nodular-iron and it's pretreatment,ascertaining laser technological parameter,spiry laser scanning strip,designing four-motor control devise using microcomputer and so on.

阐述用奥贝球铁代替 2 0Cr制造内燃机车凸轮轴的激光表面强化的工艺过程，其中包括奥贝球铁的选择、激光工艺参数的确定、螺旋激光扫描带、四联动微机控制装置的设计等。研究表明，用奥贝球铁代替 2 0Cr制造凸轮轴，经激光表面强化后，能获得更均匀的硬度分布和更高的硬度值。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。