经用

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

In this paper,the biological effects of ~(60)Co-γradiation to Bombyx mori and Antheraea pernyi were studied,and the changes of blood protein were observed through electrophoresis.

中文摘要本试验用~(60)Co-γ射线对家蚕和柞蚕生物学效应进行了研究，通过电泳试验证实了家蚕和柞蚕经辐射后体内血液蛋白质的变化。用~(60)Co-γ射线对家蚕卵进行照射，辐射剂量在10～30 Gy之间。

After arsenous acid was injected via femoral artery, arsenic concentration in serum and brain were tested by atomic absorption spectrometer.

方法用冰片对雄性Wistar大鼠灌胃给药后，经股动脉注射亚砷酸，用原子吸收光谱仪测定脑和血清中砷浓度。

Sawn, Calibrated, 4-cut and 6-cut....+ 1 mmEdges also can be chamfered / bevelled. Chamfering removes whiteness and chipped edges. This looks very good. Chamfering is a simple process in which a polishing brick is run at an angle of 45 ?.

这种类型的人造石材的生产工艺与陶瓷的生产工艺相似，是将斜长石、石英、辉石、石粉及赤铁矿粉和高岭土等混合，一般用40%的粘土和60%的矿粉制成泥浆后，采用注浆法制成坏料，再用半干压法成型，经1000℃左右的高温焙烧而成。

Abstract］ Objective To evaluate the role of PSA,PSAD（prostate specific antigen density）,FPSA and F/T prostate specific antigen percentage in the diagnosis at the prostate cancer.Methods Levels of PSA,PSAD and FPSA were examined in serums of patients with prostate cancer（56 cases） and bisectional prostate hypertrophy（149 cases）.The comparison analysis were carried to these values.

目的 评价列腺特异性抗原、游离前列腺特异性抗原、游离/总前列腺抗原百分比及前列腺特异性抗原密度在前列腺癌鉴别诊断中的价值方法对56例前列腺癌和149例前列腺增生患者用微粒酶免疫法的血清总PSA、游离PSA及游离/总前列腺抗原百分比（F/T百分比；用经直肠超声检查测得前列腺体积V，计算出PSAD值对些值进行比较分析。

Orthogonality L9(34) methods on the basis of the concentrations of caladium alkali was used to optimize the preparation technology. The final technology was determined as follow:12 times water soaking herbs, volatility oil distilled by vapor 2h, decoction 2 times every 1.5h and 85% alcohol forming granules.

经正交实验四因素三水平L9(34)方法，以西贝碱的含量为检测结果，优选了最佳的提取工艺，确定了用12倍量水浸泡4h，以水蒸汽蒸馏法收集挥发油2h，煎煮1.5h，干膏用85%乙醇制粒的科学工艺。

The proteins were purified and used to prepare the rabbit antiserum against caprine IFN-γor IL-2,the results showed that the antibody could react with the purified proteins in Western blotting.

分别用ProBond~蛋白纯化试剂盒纯化，用所获得的重组蛋白纯化产物经三次背部皮下多点注射新西兰白兔，制备了兔抗山羊IFN-γ和兔抗山羊IL-2多克隆血清。

Alloy of polyethylene of element of the methodological freeboard that make, titanium and sample of alloy of cobaltic chromic molybdenum, FITC of classics of cuticular grape coccus labels, the cuticular grape coccus that after sample of artificial and articulatory material is disinfected, vaccinal FITC labels, sample surface cent is slick surface and rough surface, every groups each 6 sample, brood the 24 orifice plate that contain bacterium and sample below 37 ℃ after Yo 30min, with fluorescent microscope observation, sample uses observation of scanning report lens.

方法制作超高分子聚乙烯、钛合金和钴铬钼合金试样，表皮葡萄球菌经FITC标记，人工关节材料试样消毒后接种FITC标记的表皮葡萄球菌，试样表面分为光滑表面和粗糙表面，每组各6个试样，将含有细菌和试样的24孔板在37℃下孵育30min后，用荧光显微镜观察，试样用扫描电镜观察。

Methods:GroupⅠ:fresh bovine pericardium was acellularized with Courtman's method and fixed with glutaraldehyde;GroupⅡ:first treated as GroupⅠ,then was modified with2,3-butanediol.

方法Ⅰ组新鲜牛心包片经Courtuman法脱细胞后用戊二醛(GA固定；Ⅱ组脱细胞及固定方法同Ⅰ组，随后用2，3丁二醇改性。

After repeated discussions and numerous consultations with many learned practitioners, we decide to follow this Buddhist tradition of building stupas and, by taking advantage of the modern manufacturing techniques, put and consecrate " The Sutra Of The Casket Seal Dharani Of The Secret Body Essence Of All Tathagatas " and " The Mantra of the Casket Seal Dharani " within these stupas.

经过反复研讨，并就教于诸方大德，我们决定用建造宝塔这一佛教传统形式，并将《一切如来心秘密全身舍利宝箧印陀罗尼经》和《宝箧印陀罗尼咒》用现代工艺制作，安奉到宝塔之中，并赋予世界和平吉祥之义。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。