经用

It,s allelchemicals were isolated through extraction, thin-layer chromatography, and column chromatograpathy. After the white clover was infused in ethanol and evaporation, the leavings was dissolved by water. The aqueous extract was extracted by petroleum,chloroform, ethyl acetate and n-Butanol successively, and the bioassay showed that the chloroform phase has obvious inhibitory effect, the inhibitory rate was 44.32%,88.08% on Abutilon theophrasti M., seedling length and root length, 82.08%,92.16% on Echinochloa crusgalli L., seedling length and root length, and the Amaranthus retroflexux L., germination rate is 0, the secondly was ethyl acetate phase.

运用萃取、薄层层析、柱层析等方法对白三叶草地上部分的化感物质进行初步分离，结果表明，白三叶草经乙醇浸提旋转蒸发后用蒸馏水溶解，依次用石油醚、氯仿、乙酸乙酯、正丁醇萃取，生物测定表明氯仿组分对杂草的抑制作用最强，对苘麻芽长和根长抑制率分别为44.32%、88.08%，对稗草芽长和根长的抑制率分别为82.08%、92.16%，反枝苋的发芽率为0；其次是乙酸乙酯相。

52 Cases of femoral intertrochanteric fracture were fixed with L-trapezoid plate,while 25 cases three cannulated lag screws with the third screw inserted at a low angle,just adjacent to medial calcar to obtain rigid fixation.

作者用切开复位，L形钢板内固定手术治疗股骨粗隆间骨折52例，用闭合复位三枚空心拉力螺钉经皮内固定治疗25例，其中第三枚螺丝钉紧贴内侧股骨矩，低角度打入。

An aliquot of the reaction mixture was injected into a C18-column with a mobile phase consisting of 0.05 molL^(-1) phosphate buffer (pH 5.5)-methanol (65:35, V/V) and detected at 235 nm.

土壤样品用0.6 molL^(-1) KOH提取，经对甲基苯磺酰氯衍生化后，注入C18柱中分离，用紫外检测器在235 nm波长检测，流动相组成为0.05 molL^(-1)磷酸盐缓冲液(pH 5.5)：甲醇＝65：35。

The surplus gold and silver materials in a unit using gold and silver may be redistributed to other units requiring it for use, subject to the consent of the local Peoples Bank of China or its agency, and at the same time the quota of allocated sales for the userunitshall be checked and reducedcorrespondingly.

使用金银单位多余的金银材料，经当地中国人民银行或其委托机构同意，可调剂给其他需用的单位使用，同时相应核减需用单位的配售指标。

Cloning of the Schwanniomyces occidentalis α-amylase and high expression in S.cerevisiae.The E.coli / yeast shuttle plasmid YCEpl partial library of Schwanniomyces occidentalis DNA was constructed and α-amylase gene fragments were screened in Saccharomyces cerevisiae by amylolytic activity.Several transformants with amylolysis were obtained and one of the fusion plasmids has about 5.0 kb inserted DNA fragment.It contains the upstream and downstream sequences of α-amylase gene from S.occidentalis .

以E.coli/yeast穿梭质粒YCEp1为载体构建西方许旺酵母部分基因组文库，在大肠杆菌中扩增后提取混合质粒DNA，经电转化非缺陷标志酒精酵母 AS.2.1364，在YPDS平板(含1%葡萄糖和1%可溶性淀粉)上用淀粉水解活力筛选含水解淀粉的阳性转化子，从阳性转化子中分离重组质粒证实含5.0kb的插入片段，用α-淀粉酶基因两端序列设计的引物PCR扩增及扩增片段序列分析证实该片段中含有α-淀粉酶全部编码序列。

The genetic inactivation of eggs is the first key stage of androgenesis in fish. In order to observe the effect of UV irradiation for the inactivation of female nucleus of zebrafish the eggs were irradiated by UV with solution-free, pure water, Ringer's solution and Cortland solution. The results showed that abnormal fry were obtained in all groups and haploids were validated. But irradiation with solution was confirmed it could not inactive fully female nucleus and the fertilization rate of the eggs irradiated with pure water was very low.

雌核灭活是雄核发育的第一个关键步骤，为研究紫外线对斑马鱼卵的雌核灭活效果，将斑马鱼的卵采用干法或置于纯水、Ringer氏溶液和Cortland鱼用生理盐水进行紫外线照射，结果表明，各种方法中均得到畸形鱼苗，并经染色体检查大多为单倍体；四种方法中在Cortland鱼用生理盐水中灭活效果最好，120 mJ/cm2紫外线可以使雌核完全灭活；干法处理不能达到雌核完全灭活的目的，在纯水中灭活后的卵子受精率较低。

Methods(1)4 cities were chosen randomly according to their economic status and geographical location.(2)An autorefractor was used to detect the diopter in the preschool children from 20 kindergartens randomly selected from each of the 4 cities.(3)1%atropine ointment was applied to the c onjunctival sac of the children with suspect myopia for 3 days and the refractio n of light in their retina was then examined by a barred beam skiascope.(4)The eye axis and corneal curvature of the myopic children were measured.(5)The diop ter,eye axis,and corneal curvature were examined every 6 months during 1-year f ollow-up after the diopter was properly rectified.

(1)依经济状况和地理位置按随机原则抽取4市县；(2)从4市县中各随机选择20间幼儿园，对所抽取的在园学龄前儿童用自动屈光仪进行屈光筛查；(3)可疑近视患者予以1%阿托品眼膏涂双眼3d，由专人用带状检影镜进行视网膜检影验光；(4)确诊为近视的患者自愿进行角膜曲率和眼轴测量；(5)近视患者经适当的屈光矫正后，按要求进行为期1年的前瞻性随访，每半年进行1次屈光状态、角膜曲率和眼轴的检测。

After repeating 5 times, the result shows that the genome DNA can be extracted from the fresh specimens by the methods ,but fail to be extracted from the preserved specimens with the same methods through electrophoresis.

经 5次重复实验，结果表明，用两种方法均能从新鲜标本中获得较完整的DNA ，且DNA得率较高；而对于无水乙醇保存的标本，提取出的DNA用琼脂糖凝胶电泳检测不出DNA条带，说明标本在保存过程中DNA可能发生了变化，致使提取出的DNA量太少或未分离出DNA 。

The auditory thresholds were examined through the auditory brainstem responses to click, which tone pips were 8, 16, and 32 kHz. The mice were intracardically perfused with 4% paraformaldehyde. The bulla were further fixed in 4% paraformaldehyde, processed and sectioned with paraffin embedded method.

用听性脑干反应测试短声、短音8，16和32 kHz听阈，经活体心脏灌注固定、听泡石蜡包埋和耳蜗连续切片，用免疫组织化学方法确定上述3种酶在内耳的分布情况。

This paper analyzed the air-conditioning system of one specific office building in Beijing, calculating the costs of unit cold and heat exergy and the unit cold and heat capacity of adopting GSHP system and air source heat pump system separately. With these numbers as standard for evaluation and comparison, it supports the conclusion that adopting GSHP system is better than adopting air source heat pump system and the heat economics analyzing method is more accurate than that based on energy.

以北京某一办公楼建筑空调系统为背景，选用地源热泵系统和风冷热泵系统两种方案，具体计算出二者的单位冷火用成本和单位冷热量成本，并以此作为评价指标，经分析比较，得出地源热泵系统方案要优于风冷热泵系统方案；以火用为基础的热经济学分析法要比以能为基础的分析法更全面准确。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。