经用

To express chicken osteoprotegerin in Pichia pastoris and determine its inhibitive effects on mature osteoclastic bone resorptive function in vitro. The chOPG cDNA encoding for 382 amino acid without signal peptice was subcloned into Pichia pastoris expression vector pPICZα-A. The constructed plasmid was transformed into yeast X-33 by electroporation. The recombinant transformants were selected by Zeocin. Induced by the addition of methanol every 24 hours, the product analyzed by SDS-PAGE was sized about 43kDa and 53kDa at a yield of 200mg per litter of culture. The result of Western blotting indicated that the recombinant protein had specific antigenicity mainly owing to heterogeneous glycosylation.

为了在毕赤酵母中分泌表达鸡骨保护素（osteoprotegerin，OPG），采用DNA重组技术将chOPG成熟肽cDNA片段插入毕赤酵母表达载体pPICZα-A中，以电穿孔法转化酵母X-33，用Zeocin 平板筛选重组子，经甲醇诱导表达后， SDS-PAGE 和免疫印迹分析表达产物，由于糖基化不同，表达产物有两种，其相对分子量分别为43kDa和53kDa，表达量约为200mg/L，经Western印迹验证，有较好的抗原性。

Shakyamuni Buddha also had used the Sutra to protect himself.

这部经是佛祖当年也用来护身的经。

Objective To study the pathogenesis and clinical symptoms of patient with central type of interˉvertebral disc protrusion manifested intermittent claudication as the mainly clinical presentation,so as to reach the early diagnosis and treamtmet in time.Methods In58cases,X-ray film,lumbar discography,CT examinations were done to confirm the diagnosis,then total laminectomig was performed to reveal the vertebal body without involving small arˉticular process.Intervertebral tissue was excised via duralotomy approach in2cases in remaining56cases,through right and left longitudinal syndesmotomy to resect the nucleus pulposus of lumber intervertebral disc.Results In58cases treated by operation,the operative findings were in according with the X-ray and CT examinations ofpre-opˉeration.

目的 对呈间歇性跛行主要临床表现的中央型椎间盘突出症患者的发病机制和临床表现进行研究，达到早诊断、早治疗方法对58例患者进行X线拍片，腰椎碘造影，CT扫描检查，根据检查结果确诊后，采用全椎板切除显露椎体，未累及小关节突，2例经硬膜切开入路切除间盘组织，余56例经从双侧用神经剥离子带棉片将硬脊膜带神经根向中线剥拉至显露圆形突出物止，分别从左右纵韧带摘除髓核。

Methods In58cases,X-ray film,lumbar discography,CT examinations were done to confirm the diagnosis,then total laminectomig was performed to reveal the vertebal body without involving small arˉticular process.Intervertebral tissue was excised via duralotomy approach in2cases in remaining56cases,through right and left longitudinal syndesmotomy to resect the nucleus pulposus of lumber intervertebral disc.Results In58cases treated by operation,the operative findings were in according with the X-ray and CT examinations ofpre-opˉeration.

对58例患者进行X线拍片，腰椎碘造影，CT扫描检查，根据检查结果确诊后，采用全椎板切除显露椎体，未累及小关节突，2例经硬膜切开入路切除间盘组织，余56例经从双侧用神经剥离子带棉片将硬脊膜带神经根向中线剥拉至显露圆形突出物止，分别从左右纵韧带摘除髓核。

Torenia fournieri genomic DNA was digested with DraI、EcoRV、PvuII、SmaI respectively. A special adaptor was ligated to the ends of the digested DNA fragments as a template for adaptor PCR. With the adaptor and TfPLC1 gene-specific primers, bands of 798bp and 813bp upstream of TfPLC1 were obtained successively.

应用衔接头PCR技术，以蓝猪耳全基因组DNA分别经DraI、EcoRV、PvuII、SmaI消化后与衔接头连接产物为模板，用衔接头引物和TfPLC1基因的特异引物经过多轮的巢式PCR，先后克隆到两个大小为798bp、813bp的TfPLC1基因上游序列；经测序、blastn比较分析和拼接得到一个蓝猪耳TfPLC1基因的启动子序列，共1432bp。

Hemodynamicvariables were assessed with transpulmonary thermodilution andtransesophageal echocardiography.

用经肺热稀释法和经食道超声心动图评估血流动力学参数。

Methods: We examined bladder with half filling of the bladder of 8695 subjects via the healthy physical examination with 5.0~7.5 MHz probe, and suggested the suspected cases perform urethrascope examination.

在膀胱少许充盈状态下，用5.0~7.5MHz的探头对8695名健康体检的人员进行常规经直肠经阴道膀胱检查，对可疑病例建议做膀胱镜检查及随诊。

After making berry pulp, being added with granulated sugar, calcium chlorid, citric acid, low-methoxyl pectin and β-cyclic-dextrin, controlling the concentrate, the low-sugar jam was processed.

用野生蓝靛果新鲜浆果为原料，经烫漂、硬化、浸糖、50℃渗糖、干燥，可制出蓝靛果低糖果脯；鲜果经打浆，加入白砂糖、氯化钙、柠檬酸、低甲氧基果胶和β-环状糊精进行控制浓缩，可制得蓝靛果低糖果酱。

For the purposes of paragraph 1 the initialling of a text constitutes a signature of the treaty when it is established that the negotiating states so agreedthe signature ad referendum of a treaty by a representative,if confirmed by his state,constitutes a full signature of the treaty.

此句中的目的状语简练短小，不妨就用&就&字把其放于句首：就适用第一项而言：倘经确定谈判国有此协议，约文之草签构成条约之签署；代表对条约作核准之签署，倘经其本国确认，即构成条约之正式签署。

In this paper, purification of growth hormone receptor from ovine liver and identification of its biochemical character have been done.Firstly, by homogenizing, triturating, high-speed centrifuging, hypervelocity centrifuging and sucrose density grade centrifuging cell membrane of ovine liver was separated and its electron microscope slice wasprotein was disL~olved with TritonX-100,amL-thebinding activity between cell membrane or membrane protein and ~I-hGH using RRAwas measured, perfect saturated curve wasgained. Thirdly, specific binding of GILR protein was measured ,by SDSPAGE, its molecular weight is about 1 19KD.

本研究采取新鲜羯羊肝脏，匀浆、研磨，经高速离心，超速离心和蔗糖密度梯度离心，分离肝脏质膜，得到肝脏质膜的电镜切片：其次，用去垢剂TritonX-100增溶膜蛋白，利用放射受体分析法，测定细胞膜及膜蛋白与~(125)I-hGH的结合活性，均得到理想的饱和曲线；再次，将两点结合试验法与凝胶层析法结合检测到特异结合物，然后，经SDS-PAGE电泳分析，纯化后的样品呈现一条带，与标准分子量比较，得出分子量为119KD。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。