经用

In order to develop antibacterial peptide biologics for prevention and control of endometritis of dairy cow,a pair of primers was designed and synthesized according to the sequence of bovine antibacterial peptide gene published in GenBank.The total RNA was purified from the dairy cow endometrial cells with the Total RNA Isolation Kit.

为研发防治乳牛子宫内膜炎的抗菌肽生物制剂，根据已发表的牛抗菌肽基因序列设计了1对引物，用试剂盒提取乳牛新鲜子宫内膜总RNA，经RT-PCR扩增，将克隆的抗菌肽BNBD5基因全长编码区的cDNA连接到原核表达载体pET30a中，经IPTG诱导原核表达，通过Western-blot检测融合蛋白BNBD5的表达水平。

And then the two different turfgrass seed germination tests conducted, results show that: 0.1 percent concentration of hydrogen peroxide concentration of 0.01% of the "Bi-care" and the concentration of 0.29 percent for the "God LU Hong Lu," The three kinds of reagents soaking treatment can significantly improve the wild Pennisetum and Eragrostis seed germination and growth of 0.10 percent by the concentration of the soaking H2O2 solution, then 0.29% concentration of the "God LU Hong Lu" after soaking solution, wild Pennisetum Eragrostis seeds and seed germination energy, germination percentage, germination index, root length and were significantly higher than control to deal with the seeds, but also to varying degrees higher than the other chemical reagents used to deal with the seeds, rapid germination and tidy.

然后对两种草坪草不同的种子处理进行了发芽试验，结果表明：浓度为0.1%的过氧化氢、浓度为0.01%的&碧护&和浓度为0.29%的&鲁虹神露&这三种试剂浸种处理能显著提高野生狼尾草和大画眉草种子的萌发和生长，经浓度为0.10%的H2O2溶液浸种，再经浓度为0.29%的&鲁虹神露&溶液浸种处理后，野生狼尾草种子和大画眉草种子的发芽势、发芽率、发芽指数、根长和芽长均显著高于空白对照处理的种子，也不同程度的高于用其他化学试剂处理的种子，发芽快而整齐。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的，我们设计了以下的实验步骤﹔（1）二次代谢物回收及检测样本处理：我们将发酵所得的培养上清液，利用疏水吸附性树酯Amberlite XAD-2吸附回收，并以甲醇溶离及真空减压浓缩脱水等方式处理，以取得提供抗氧化活性筛选之检测样本；（2）抗氧化活性检测及菌种筛选：以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用；经此筛选程序，我们选获了具有抗氧化物质高生产力的菌株，命名为AMBL-029C；（3）抗氧化物质的分离纯化：针对生产菌株的发酵回收处理液，以矽胶薄层色层分离法经物质层析纯化后，并以高效能液相层析法(High performance liquid chromatography；HPLC)分析物质可得一初级纯化物质，命名为AMBL-029C-TS；（4）抗氧化物质的物理化学性质分析：由酸碱转溶（acid-base fractionation）实验得知，此抗氧化物质属於中低极性的强酸性物质，对温度（37℃-100℃）及酸碱度（pH3.0-13.0）均表现出高稳定性；（5）在抗氧化机制探讨方面，我们针对数种不同的抗氧化机制进行探讨，即： 1。

Results Out of the4527paˉtients treated by ESWL,stones were excreted completely from4512patients within2weeks;12patients who had comˉplication of the urethra stone street were cured by transcutaneous nephrocentesis,nephrostomy and ureterolithotomy through uretercystoscope;stones weretaken out by operation from the other3patients,from whom the excretion of stones was not possible.

结果 经ESWL治疗的4527例泌尿系结石病人中，4512例在2周内排尽结石，12例并发输尿管&石街&，加用经皮肾穿刺造瘘和输尿管镜取石治疗，3例中转手术治疗。

Methods Undertake the technique of parotid sialography with48%Lipiodol ultra-fluideunder X-ray upon78patients who were given systemic anti-infections and supporting treatments only with non-obvious-results,and classify all the cases into chronic obstructive(21cases)and nonobstructive parotitis(57cases)accoding to the results of sialography through microcatheter,then go on...

根据影像所见，将患者分为慢性阻塞性(21例)和慢性非阻塞性(57例)腮腺炎。造影前，经微导管引流腮腺内分泌液，做细菌培养及药敏试验。对慢性阻塞性腮腺炎病人，给予经导管向腮腺内灌注2%利多卡因、1%甲紫治疗。对慢性非阻塞性腮腺炎病人，用同样方法注入α-糜蛋白酶、丁胺卡那霉素、生理盐水混合液治疗。结果慢性阻塞性腮腺炎组治愈率为80.95%，慢性非阻塞性腮腺炎组治愈率为87.72%。结论造影-介入是诊断和治疗慢性化脓性腮腺炎确切有效的方法。

In a cross-sectional study, the researchers used dual energy X-ray absorptiometry to compare measures of body composition and BMD in 28 girls with Turner syndrome who had neer receied GH and 39 girls who were treated with GH for at least 1 year. Hand radiography was used to compare phalangeal cortical thickness.

在一个代表性的研究中，研究人员利用双能X-线吸收仪分别测量28个没经生长激素治疗的患有Turner综合症的女孩和39个经生长激素治疗至少一年的患有Turner综合症的女孩的机体组分和骨矿物质密度，同时用手X线机测量她们的指皮质厚度。

The total synthese three natural compounds to explore enantioselective synthesis of flavanol: We use phloroglucin and 2, 4, 6-trihydroxyacetophone as starting materials to synthesis distenin in two ways; Resorcin was used to synthsis 2, 5-dimethoxymethoxyacetophone though 3 steps.

对黄烷醇化合物的立体选择性全合成进行了探索：以间苯三酚和2，4，6-三羟基苯乙酮为原料，尝试用两条路线对Distenin化合物进行了全合成研究；以对苯二酚为原料，经三步制得2，5-二甲氧甲氧基苯乙酮，与2，4，6-三甲氧甲氧基苯甲醛反应，经四步反应得到天然产物3，5，7，2′，5′-二五羟基黄烷关键中间体1-（2′，6′-二甲氧甲氧基苯）-3-（2&，4&，6-三甲氧甲氧基苯）丙烷-1R，2R-二醇83及其对映体84；简要介绍了EGCg类化合物的生物活性和研究概况。

Pyridazine was prepared by hydrogenolysis of 3,6-dichloro pyridazine obtained via chlorination of 3,6- pyridazine diol with phosphorus oxychloride.

以顺丁烯二酸酐与盐酸肼为原料经缩合成环反应生成顺丁烯二酰肼，再用三氯氧磷氯化合成3，6-二氯哒嗪，最后经催化加氢制得标题化合物哒嗪。

Thirty-five patients with carcinoma of the lower rectum were completed the cutting operation passing the belly in Dixon operation, clamping the sigmoid at the 5 cm of location for anastomosis passing anus to insert one piece of 22# foley urea pipe, using 15-20 mL of water filling into urea pipe ball bag, then pulling urea pipe to stop with clear blockage, and passing urea pope forward recta cavity to fill 100-150 mL of methylene blue dilution to observe the opening of anastomosis.

探讨直肠癌行低位Dixon手术，术中采用肠腔封闭式加压灌注检测及术后放置支架管引流对吻合口瘘的预防作用。1本组低位直肠癌患者经腹腔完成直肠癌直肠前切除吻合后，用肠钳在吻合口上端5cm处钳夹肠管，再经肛门插入1条22#Foley尿管，尿管球囊注入15~20mL水后向外拖动尿管至有明显阻力时停止。

Liang Qi-chao,Hu Shi and Shen You-ding applied western logicto compare Mo-jing logic and made Mo-jing logic refound.

梁启超、胡适、沈有鼎等人通过研究西方逻辑，从而用来比较研究墨经逻辑，才使墨经逻辑重新被发现。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。