经用

Wild baccae of lonicera edulis was used as raw material.

用野生蓝靛果新鲜浆果为原料，经烫漂、硬化、浸糖、50℃渗糖、干燥，可制出蓝靛果低糖果脯；鲜果经打浆，加入白砂糖、氯化钙、柠檬酸、低甲氧基果胶和β-环状糊精进行控制浓缩，可制得蓝靛果低糖果酱。

The data of mechanical properties test proved that the composite materials compounded with 0.39~0.46um PS organic rigid particles at 0.2% and PP have excellent strength and toughness. SEM photograph found that the organic rigid particles were separated into 60~80nm size in the composite by melting process, which has belter toughness compare with the PP compounded with ultra-fine organic rigid particles.

用2％粒径为0.39～0.46um的XPS复合PP，经力学性能测试，有最好的增韧效果；SEM扫描电镜发现，经热加工的复合材料中最小的有机刚性粒子粒径可部分分散到60～80nm，该复合材料比超细有机刚性粒子复合的PP有更好的冲击韧性。

Methods Fourteen healthy pigs were anesthetized and intubated, the right carotic artery was cannulated for blood pressure monitoring and blood biochemical sampling.

术中输入不含糖的平衡盐液，供肝用Collin's液灌注。在无肝期采用体外静脉转流，经左侧股静脉和门静脉插管，引流出的血流经体外转流系统输入左侧腋静脉。

A polyclonal antibody against vitellin was prepared in male rabbits and the specific antibody against vitellin was made through absorbtion by male worm homogenate. After purification, HRP was used to mark antibody, then ELISA was set up to detect vitellin content in the Chrysopa septempunctata fat-body, hemolymph and ovary.

提取了大草蛉的卵黄蛋白，免疫家兔，并将所得抗血清经雄虫匀浆液吸收，获得抗卵黄蛋白的特异抗体，经纯化后，用辣根过氧化物酶标记抗体，建立了ELISA法测定大草蛉脂肪体、血淋巴和卵巢中卵黄蛋白含量的方法。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

In the third part of this paper, a facile method is presented for synthesis of malonaldehyde dianil from propargyl alcohol by oxidizing using chromium trioxide and addition reaction with aniline.

对肼基苯磺酸是由对氨基苯磺酸经亚硝酸钠重氮化得到的重氮盐用亚硫酸氢钠还原、水解、硫酸酸化制得，本文经多次实验确定了反应原料、用量及反应条件，得到的产品纯度好，产率高。

Hydroxy-5, 10-dihydrophenophosphazine 10-oxide (1) reacted with SOCl2, and then with MeONa in MeOH to give a methyl phosphinate (2) which can be converted to a dinitro compound 3 (62%) by nitration and the resulting dinitro compound 3 can further be converted to 2, 8-diamino-10-methoxy-5, 10-dihydrophenophosphazine 10-oxide (4, 71%) by hydrogenation over Pd/C (w = 0.05). And the effect of amount of Pd/C (w = 0.05) on the reduction was also studied.

10－羟基－5，10－二氢磷杂吖嗪－10－氧化物（1）经醯氯化、甲基化得到10－甲氧基－5， 10－二氢磷杂吖嗪－10－氧化物（2），2经硝化得到10－甲氧基－2，8－二硝基－5，10－二氢磷杂吖嗪－10－氧化物（3，62%）。3在Pd／C（w = 0.05）存在下，用氢气还原为2，8二氨基－10－甲氧基－5，10－二氢磷杂吖嗪－10－氧化物（4，71%），考察了Pd／C（w = 0.05）用量对氢化还原反应的影响。

Other three alien translocation lines referring to chromosome Lr.14 of L.racemosus were also induced via irradiated pollens.After irradiated by gamma raysof 1KR does,the pollens of T.aestivum-L.racemosus Lr.14 disomic addition lineswere used to pollinate wheat varieties"Yangmai No.5"and"Mianyang No.11".M1plants have pairing between wheat chromosomes and Lr.14 ofL.racemosus at PMCMI stage were self-pollinated,and the M2 seeds used to be Giemsa C-banded andfluorescence in situ hybridized.As the result,three alien translocation lines wereobtained and T12 was observed to have distal translocation chromosomes betweenLr.14S and a wheat chromosome segment(TW-Lr.14S·Lr.14L).T13 wasidentified to contain mostly-alien-translocation chromosome(TLr.14L·Lr.14S-W).T14 was proved to contain a translocation between Lr.14L and 6BS,with approximatebreakpoint at 6BS2.2 region, FL0.22 in the short arm of chromosome T6BL.6BS-Lr.14L.

用普通小麦-大赖草Lr.14二体异附加系经辐射处理过的花粉给扬麦5号和绵阳11号授粉，M1代植株进行PMC MI染色体配对分析和Giemsa C-分带，选出PMCMI小麦和大赖草染色体发生配对的植株进行自交，M2代经RTC M期染色体C-分带和原位杂交，鉴定出3个小麦-大赖草易位系，其中T12为由Lr.14大部分和一个小麦染色体片段组成的易位染色体(TLr.14L·Lr.14S-W)；T13含由Lr.14染色体绝大部分与小麦染色体的一个小片段组成的易位染色体(TLr.14L·Lr.14S-W)；T14易位系的染色体易位发生在小麦6BS2.2区和大赖草Lr.14L之间，易位断点在T6BL·6BS-Lr.14L易位染色体的FL0.22附近。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。