经

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果：倒置显微镜和透射电镜观察显示，经SAHA处理的细胞增殖速度显著减慢，细胞体积增大，细胞核较小，形状较为规则，核仁数量减少、体积变小，核内常染色质增多而异染色质减少，核质比例减小，细胞质内线粒体数量增多、线粒体嵴发达，高尔基体较为典型，粗糙型内质网增多，呈现出与正常上皮细胞相似的形态变化；MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用，并有明显的剂量依赖和时间依赖关系；免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达；流式细胞仪检测结果显示，SMMC-7721细胞经SAHA处理后，G0/G1期细胞明显增加，S期细胞则明显减少，细胞被阻滞于G0/G1期；RT-PCR检测结果表明，SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加，24h后更为明显。

The nanoplex were smaller than the unmodified nHAP; thelipiodol/nanoplex emulsion, with the mean diameter at 75±11.8nm can disperse moreuniformly and be more soluble and stable, contrary to the unmodified nHAP and nanoplex Conclusion: Nanoplex/pDNA mass ratios greater than 15 had exhibited completepDNA absorption onto positive charged polyplex and pDNA protection from thedestruction of nucleinase in rabbit serum. Unmodified nHAP may can\'t induce effectivegene delivery.PartⅡThe construction of the recombint eukaryotic expressive vector ofwild-type human p53 and RbObjective: To construct the recombint eukaryotic expressive vector of wild-type humanp53 and Rb by utilizing the theory of molecular medicine.

结果nHAP颗粒本身大小小于100nm，但由于颗粒表面本身带负电，不能包括有效地结合及保护DNA；经Ca~(2+)修饰后，颗粒可带正电，可一定程度地结合和保护DNA，但聚合物本身的中性电与带负电的细胞膜结合，而且本身的颗粒则变地容易聚集而成为很大的颗粒，也不利于细胞的吞噬；经PLL修饰后，颗粒溶解性提高，直径变小，表面带正电，电泳结果显示PLL-nHAP颗粒可包括有效地结合和保护DNA免受兔血清的破坏，而且复合物表面的正电也包括有利于与细胞膜的结合与吞噬。

A total of 304 patients were willing to choose Gongle IUD,of which 280 parous women and 24 nulliparous women were found no insertion contraindication following the gynaecological examination and the examination of vaginal discharge.

304例受术者均为自愿选择宫乐，其中经产妇280例，未孕妇24例，经妇检及阴道分泌物检查无放置禁忌证。放置后1、3、6、12

The various treatment options available for these patients are isolated C1–C2 fusion, occipitocervical fusion with or without transoral surgery.

治疗选择多样有单独的寰枢椎融合，枕颈融合，经口或不经口的手术。

Objective: To clarify the technical parameters in posterior atlanto-occipital transarticular screw fixation and discuss the clinical significance of occipitocervical junction fixation with transarticular screws.

目的：明确后路寰枕关节经关节螺钉固定的技术参数；探讨枕颈部经关节螺钉内固定技术的临床意义。

Text of Bhaddekaratta read as "Pandita' in Madhyamagama 34th Sutra (See Vol. I, Taisho Tripitaka p. 886 B). It reads "A single Excellent Night" in the English translation of Majjhima-nikaya 132nd Sutra; notes in the FoGuangShan Edition translated as "A single worthy night", which elaborated as "one who living in a worthy life day and night p.

三、「跋地罗帝」，《佛说尊上经》作「贤善」（大正大藏经第一册第八八六页中），《中部第一三二阿难一夜贤者经》作「一夜贤者」，英译本作「A single Excellent Night」，佛光本注解译为「贤善一夜」，演义为「夜夜全然如是贤善生活之人」。

In conclusion, antisera collected from skin graft immunized cattle had higher antibody titer but with more cross-reaction than sera collected from parous cows. The cross-reaction of alloantisera from skin graft immunized animals existed although they had been diluted and absorbed.

综观二种方法之比较，经由筛选经产母牛血清而制得之标准抗血清，其抗体力价偏低，然其交叉反应情形较少，特异性较高；而经由皮肤移植及埋植方法免疫而获得之抗血清力价虽高，但经用稀释及吸收方法仍难避免交叉反应。

Results By celioscopic diagnosis, 109 females (54.5%) found ill with chronic pelvic inflammatory disease, 55(27.5%) with endometriosis 18 with phase Ⅰ~Ⅱ, 37 with phase Ⅲ~Ⅳ, 12(6% with polycystic ovarian syndrome, 8(4%) with pelvic tuberculosis and 4(2%) with malformation of internal genitalia. The other 12 ones (6%) had normal pelves.

结果 200例中经腹腔镜诊断为慢性盆腔炎并输卵管病变109例(54.5%)；子宫内膜异位症55例(27.5%)，其中Ⅰ~Ⅱ期的18例，Ⅲ~Ⅳ期的37例；多囊卵巢综合征12例(6%)；盆腔结核8例(4%)；内生殖器畸形4例(2%)；正常盆腔12例(6%)。60例经腹腔镜治疗后妊娠(30%)。

The analysis of X-ray diffraction and scanning electron microscope energy dispersion spectra show that the primary hydrolysis products of antimony pentachloride and its hydrochloric acid solution are amorphous antimony oxide rather than SbO2Cl.

X射线衍射和扫描电镜能谱分析表明：五氯化锑及其盐酸溶液的初始水解产物不是SbO2Cl，而是一种非晶态锑氧化合物；随着水解母液酸度的降低，五氯化锑溶液水解产物陈化五天后的晶型由非晶态向晶态变化；五氯化锑溶液水解产物经无水乙醇洗涤后水解产物仍为非晶态，而经蒸馏水洗涤虽主要为非晶态，但有少部分结晶的Sb2O5·4H2O晶体存在。

Objective To study the therapeutic efficacy of percutaneous implantation of ~(125)Iodine seeds, Methods Under guiding of untrasound,~(125)Iodine seeds were implanted percutaneously into pancreatic cancerous masses.

目的研究经皮埋置~(125)碘籽对不能手术切除性胰腺癌的治疗价值。方法在B超监视下，经皮穿刺注入~(125)碘籽人胰肿瘤内。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。