经

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Background— Mild commissural mitral regurgitation is associated with significantly higher restenosis-free surial after percutaneous mitral aluloplasty, which suggests that different mechanisms of significant MR after PM may hae different clinical courses.

背景：经皮二尖瓣成形术后严重高度非再狭窄存活率与轻度交界处损伤的二尖瓣关闭不全相关，表明经皮二尖瓣成形术后严重二尖瓣关闭不全的不同机制可能有不同的临床过程。

Background— Mild commissural mitral regurgitation is associated with significantly higher restenosis-free surial after percutaneous mitral aluloplasty, which suggests that different mechanisms of significant MR after PM may hae different clinica

背景：经皮二尖瓣成形术后严重高度非再狭窄存活率与轻度交界处损伤的二尖瓣关闭不全相关，表明经皮二尖瓣成形术后严重二尖瓣关闭不全的不同机制可能有不同的临床过程。

The electron microscope scanning results showed that dense mycelia and conidial colonies were observed on the leaves of non K2SiO3-applied plants, while loosened mycelium or no mycelium on the leaf surface of K2SiO3-applied plants.

经电镜扫描分析显示，诱导接种后，未加硅酸盐的叶片布满浓密的菌丝体及分生孢子团，经硅酸盐处理的叶片上仅有松散的菌丝或者没有菌丝分布。

Methods Two or more than two methods of patholoˉgy,cytology,B-ultrasound,CT,MRI,ECT,tomography,surgical dissection verified the diagnosis of202cases of panˉcreatic cancer,who were inpatient.

202例住院胰腺癌病人经病理学、细胞学、B超、CT、MRI、ECT、摄片、手术探查等两项或两项以上手段证实，并经X 2 检验。

The results showed that the D50 of Astragalus membranaceus and Epimedium brevicornum ultra-fine powder were 6.82μm and 8.40μm separately. The broken cellular walls of two ultra-fine herbal powder were bigger than those of the commonly cribble, and intact cells were not visible under SEM, and it improved the mixed uniformity. On the contrary, organization blocks of the commonly cribble were clearly discernible, also the shape and granularity were irregular, and the organization cells were still complete. The bulk density and liquidity of Astragalus membranaceus and Epimedium brevicornum increased significantly after ultra-fine Smashing. The component icariin extraction content of the ultra-fine powder was enhanced by 18.31% compared with the cribble by the chromatograph analysis of icariin. At the same time, Astragalus membranaceus polysaccharide and Epimedium brevicornum polysaccharide content of the ultra-fine powder were enhanced by 117.13% and 315.15% compared with the cribble.

结果发现黄芪和淫羊藿经超微粉碎后，粉体的粒度分布中心D_(50)分别为6.82μm和8.40μm，经电镜观察均难以看到完整的细胞形态，只能看到细胞碎片，破壁率远远大于普通粗粉，其混合的均匀度显著提高，而普通粗粉在显微镜下，组织块清晰可见，且形状不规则，粒径分布不均匀，组织细胞仍然完整；超微粉碎后，黄芪和淫羊藿的堆密度和流动性显著增强；通过对淫羊藿有效成分淫羊藿苷的色谱分析，其中淫羊藿超微粉的淫羊藿苷体外溶出量较淫羊藿粗粉中提高了18.31%；同时黄芪、淫羊藿超微粉多糖的提取率分别较黄芪、淫羊藿粗粉提高了117.13%，315.15%。

The proliferation of thyroarytenoid muscle and lateral cricoarytenoid muscle cell of dog remained at high level in 5 months after denervation and then slowed down and atrophy of muscles occurred 6 months after denervation.

甲杓肌及环杓侧肌失神经支配后5个月内保持高增殖水平，以后均降低，失神经支配6个月后肌萎缩开始明显。

Result :There was no significant difference between sexes,ages and sides.The fibrillation potential amplitude was maximum at 3 to 4 months after denervation of the posterior cricoarytenoid muscle or at 5 to 6 months after denervation of the thyroarytenoid muscle and still remained at certain level for years in some patients.Significant difference was showed in complete and incomplete nerve injuries groups,but changes of amplitude with time in incomplete nerve injuries group were small.

结果：不同性别、年龄及不同侧的纤颤电位波幅间的差异无显著性，失神经环杓后肌纤颤电位波幅均值在病程2～＜4个月时最高，而失神经甲杓肌纤颤电位波幅均值在病程4～＜6个月时最高，相当一部分患者的纤颤电位波幅晚期仍维持一定水平，并且神经完全损伤者和不完全损伤者各时间纤颤电位波幅均有显著性差异，而神经不完全损伤者各时间幅度变化小。

Route of Administration:Inhalation, Orally,Cutaneously

染毒途径：吸入，经口，经皮

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。