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At the same time, intracellular DNA was extracted and subjected to Southern blot, hybridized with a ~(32)P-labeled HBV probe and autoradiographed. The intensity of the autoradiographic bands was quantitated by densitometric scans of computer and ED_(50) was calculated.

然后以2.2.15细胞培养为基础,用不同浓度的β-L-D4A进行干预后,ELISA检测上清液中HBsAg及HBeAg的含量,提取病毒DNA后进行Southern印迹、~(32)P标记HBV探针杂交、放射自显影,计算机图像处理进行定量分析,求出50%抑制的药物浓度,即EC_(50)。

Results:β-L-D4A was successfully synthesized and confirmed by IR, NMR and MS. Autoradiographic bands showed similar for supernatant and intracellular HBV DNA. Episomal HBV DNA was inhibited in a dose-dependent manner. EC50 of 3 -L-D4A was 0.2 μM. The experiment of cytotoxicity gained its IC_(50) 200 μM.

结果一系列步骤合成的化合物β-L-D4A红外光谱、质谱和核磁共振谱鉴定正确;2.2.15细胞培养上清液病毒DNA的Southern印迹、自显影结果显示药物对病毒的抑制呈明显的浓度依赖性,计算出EC_(50)为0.2μM;细胞毒性实验显示IC_(50)为200μM。

All the three yin meridians of the hand come out of the axillae without exception, all the three yin meridians of foot run along the entral aspect.

所有的手三阴无一例外的起于腋窝部,所有的足三阳沿腹部循行。

All the three yin meridians of the hand come out of the axillae without exception, all the three yin meridians of foot run along the ventral aspect.

所有的手三阴无一例外的起于腋窝部,所有的足三阳沿腹部循行。

Dissections were performed on 1.2cm interval cross-sections of the thorax of 30 embalmed cadavers. The results showed 20.00±7.30%of the aortic arch and arch of the azygos vein at the same plane and 40.00±8.94%of the arch of the azygos vein at the level of concavity of the aortic arch.

为描述和阐明主动脉弓平面横断层、纵隔内结构的解剖学特征,为医学影像CT、MRI提供解剖学依据,把防腐固定的30具成年男性尸体制成间距为1.2cm的连续横断层标本,进行观察。

Wild baccae of lonicera edulis was used as raw material.

用野生蓝靛果新鲜浆果为原料,烫漂、硬化、浸糖、50℃渗糖、干燥,可制出蓝靛果低糖果脯;鲜果打浆,加入白砂糖、氯化钙、柠檬酸、低甲氧基果胶和β-环状糊精进行控制浓缩,可制得蓝靛果低糖果酱。

The results showed that the carbon membranes preoxidized at 460℃,30min,and carbonized at 650℃reached the highest permeability.The permeabilities of H_2,CO_2,O_2,N_2 were 1016.4、710.3、187.8、14.0 Barrer1 Barrer=10~(-10cm~3·cm·cm~2·s~(-1)·cmHg~(-1),respectively.The carbon membranes preoxidized at 460℃,30 min,and carbonized at 850℃reached the highest selectivity.The ideal separation factors of H_2/N_2、CO_2/N_2,O_2/N_2 were 129.2、93.1、21.9、respectively.

结果表明:PPESK前驱体460℃,30 min预氧化处理,650℃炭化得到的炭膜气体渗透性最佳,H_2、CO_2、O_2、N_2的渗透系数分别达到了1016.4、710.3、187.8、14.0 Barrer1 Barrer=10~(-10cm~3·cm·cm~(-2)·s~(-1)·cmHg~(-1);460℃,30 min预氧化处理,850℃炭化得到的炭膜气体选择性最佳,H_2/N_2、CO_2/N_2、O_2/N_2的分离系数分别达到了129.2、93.1、21.9。

It was found using microwave could eliminate the lesion located at the medial, basial and posterolateral walls of maxillary sinus through enlarged ostium completely and avoid the trauma caused by the operation via the labiogingival groove approach.

发现采用微波热凝术扩大自然开口能顺利地清除上颌窦腔内、底壁及后外侧壁等部位的病变,避免了病变组织的残留和唇龈沟径路手术的创伤,提高了ESS的疗效。

FEATURES: Hardened and precision-ground bedways, the spindle is supported with precision taper roller bearings, gears in the headstock are made of high-quality steel, hardened and ground, speed-changing levers driven by fan-shaped gears, operating easily, safe interlock and a overload safety devices in apron, electromagnetic braking, cooling and lighting devices are supplied.

特点:床身导轨表面淬火和精密磨削,主轴支撑采用精密圆锥滚子轴承,主轴箱内齿轮采用优质钢并表面淬火和磨削,变速机构采用扇型齿轮传动,操作灵活方便,溜板箱设有安全互锁装置和过载保险机构,配有电磁刹车、冷却及照明装置。

The data of mechanical properties test proved that the composite materials compounded with 0.39~0.46um PS organic rigid particles at 0.2% and PP have excellent strength and toughness. SEM photograph found that the organic rigid particles were separated into 60~80nm size in the composite by melting process, which has belter toughness compare with the PP compounded with ultra-fine organic rigid particles.

用2%粒径为0.39~0.46um的XPS复合PP,力学性能测试,有最好的增韧效果;SEM扫描电镜发现,热加工的复合材料中最小的有机刚性粒子粒径可部分分散到60~80nm,该复合材料比超细有机刚性粒子复合的PP有更好的冲击韧性。

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Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

However, to get a true quote, you will need to provide detailed personal and financial information.

然而,要让一个真正的引用,你需要提供详细的个人和财务信息。