经

The proving up and exploiture of the carbonic and niton hotspring have been drafted, which have been approved Emei Plan Economy Development [2001] No.

峨眉山市计经局峨计经发[2001]199号文件批准建设，已完成项目规划设计、方案设计、可行性研究。

Results: Porphyrin nitrogen mustard (5 μg/ml ) decreased the D value of human liver cancer cell line SMMC7721. The activity of LDH was increased after laser irradiation. The3H-TdR of human liver cancer cells was decreased with or without laser.

结果：卟啉氮芥(5μg/ml，培养1h)可使人肝癌细胞SMMC7721D值明显降低、经光激发可使培养上清LDH活性显著升高；可使体外培养人肝癌细胞SMMC7721的3H-TdR掺入量显著下降、经光激发可使体外培养人肝癌细胞SMMC7721的3H-TdR掺入量进一步显著下降。

Article 9 Licensees shall uninstall all of the hot spring facilities and take proper action for restoration or remediation under any of the following situations: failing to capture hot spring water, or cancellation or revocation of the permit, or nonuse of hot spring for more than one year.

第9条经许可开发温泉而未凿出温泉或经直辖市、县主管机关撤销、废止温泉开发许可或温泉停止使用一年以上者，该温泉取供事业应拆除该温泉有关设施，并恢复原状或为适当之措施。

Objective To evaluate the feasibility of auxiliary orientation by X ray with an oblique angle in percutaneous nephrolithotomy.

目的探讨在经皮肾镜取石术中应用X线斜向辅助定位法建立经皮肾通道的可行性。

Methods Methods Tension-free vaginal tape Obturator was applied for 8 women with stress urinary incontinence.

采用经闭孔经阴道无张力尿道中段悬吊术治疗女性压力性尿失禁8例。

In our experiment, the GADes gene from rat brain was cloned and expressed in E.coli, so as to make the recombinant GADes produced by gene technology for clinical application and further study on the relation between GAD and type 1 diabetes mellitus.Firstly, with the pUClS/GADes recombinant plasmid as template, the full-length encoding sequence of GADes was amplified by PCR, and cloned into pGEM-T vector. Secondly, the target gene was cut by EcoRI and inserted into restriction sites of pET42a vector for expression.

本研究首先用PCR方法，以pUC18/GAD_(65)为模板，扩增目的基因，克隆到pGEM-T载体中，构建pGEM-T/GAD_(65)重组质粒，然后用EcoRI酶切，切下的目的基因片段插入pET42a融合型表达载体中，经酶切鉴定和测序，证实插入方向、读码框架正确；重组表达质粒转化大肠杆菌BL21(DE_3)中诱导表达，表达产物经亲和层析纯化后获得了融合蛋白GST-GAD_(65)。

Methods The total RNA was extracted from the brain of human embryo. The segment of open read frame of DOC-1 was amplified by RT-PCR and was further cloned into the vector, pMD18-T, and the prokaryotic expression vector, pGEX-4T-1, by turns, to produce the new construct pGEX-4T-1-DOC-1. After restriction enzymes digestion analysis and sequenced, pGEX-4T-1-DOC-1 was transformed into E.coli BL21（DE3）. GST-p12 recombinant protein was expressed under IPTG induction and purified by affinity column.

从人胎脑组织中提取总RNA，经逆转录-聚合酶链式反应扩增DOC-1的CDS序列，再通过基因重组技术将该基因片段依次克隆到pMD18-T和pGEX-4T-1载体中，构建融合表达载体pGEX-4T-1-DOC-1，经酶切、测序鉴定后，用该重组质粒转化E.coliBL21，用IPTG诱导表达，Glutathione Sepharose 4B柱亲和层析纯化重组蛋白。

Please attach a name list of the board of directors, an organisation chart, organization's Constitution or Articles and Memorandum and the audited accounts of the organisation of

请夹附机构董事会成员名单、机构管理架构图、机构组织章程的详细资料，以及机构上一年的经审计帐目或最近经核证的管理帐目。

Iv a name list of the board of directors, an organisation chart, organisation's Constitution or Articles and Memorandum and the audited accounts of the organisation of the previous year or certified management accounts (for NGOs currently not receiving SWD subventions only); and

iv 贵机构董事会成员名单、机构管理架构图、机构组织章程的详细资料，以及机构上一年的经审计的帐目或最近经核证的管理帐目(只适用於目前并无接受社署资助的非政府机构)；以及

Please attach a name list of the board of directors, an organisation chart, organisation's Constitution or Articles and Memorandum and the audited accounts of the organisation of the previous year or certified management accounts, please check the checklist in Item 7 for documents submission.

请夹附机构董事会成员名单、机构管理架构图、机构组织章程的详细资料，以及机构上一年的经审计帐目或最近经核证的管理帐目，请参阅第七项递交文件核对清单。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。