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Methods Microsatellite instable-high colon cancer cell line HCT116, microsatellite stable colon cancer cell line HT29, as well as control MSS breast cancer cell lines were treated by NMD inhibition drug emetine and actinomycin D.

方法采用NMD抑制药物emetine联合actinomycinD,分别处理高度微卫星不稳定型大肠癌细胞株HCT116和微卫星稳定的大肠癌细胞株HT29,以及对照的MSS乳腺癌细胞株,以全基因组cDNA芯片检测药物处理和不药物处理的细胞株的mRNA表达变化。

The endermic LD50 to rat and mouse were 926 mg/Kg and 1210 mg/Kg respectively. The results of subscute toxicity test in rats showed that the content of blood urea nitrogen in exposure groups was higher than that in control group, the histopathological changes of testis observed in exposure groups were obvious. Mutagenic tests including bacillus subtilis DNA rec-assay, micronucleus ...

其大、小鼠皮LD_(50)分别为926mg/kg和1210mg/kg;大鼠90天皮染毒,试验组BUN含量高于对照组,光镜下,试验组睾丸的组织学结构有明显改变;枯草杆菌DNA重组试验、小鼠骨髓多染红细胞微核试验和小鼠睾丸染色体畸变分析试验均获得阳性结果。

A PCR product was amplified using RT-PCR method from total RNA from the abdomens of R strain using the degerated primer. The PCR product was purificated and cloned. The plasmid is pBluescript Ⅱ KS. Recombined plasmid DNA was identified by endoenzyme. The results indicated there were different P450 genes in the PCR product.

运用这对简并引物首次从德国小蠊抗拟除虫菊酯的品系R中扩增出的特异性的DNA片段,纯化、连接和转化,重组至pBS质粒,筛选阳性克隆,提取重组质粒的DNA,并初步的酶切鉴定,发现存在着不同的CYP4基因。

Observations by laser confocal microscope and determinations by electron probe revealed that neither carbonaceous chondrite nor enstatite chondrite were found among these meteorites.

激光共聚焦显微镜观察,未查出碳质球粒陨石。电子探针鉴定,也未发现顽火辉石球粒陨石。

Methods of stem cell transplantation for acute myocardial infarction included direct epicardium and endocardium injection, intravenous infusion and coronary catheter injection, of which coronary catheter injection was a prospective approach that was direct and with small wound.

干细胞移植治疗急性心肌梗死的方法主要有直接心外膜、心内膜注射、静脉输注和冠状动脉导管注射4种形式,其中冠状动脉注射较为直接,创伤小,可能是最有前途的移植途径。

Result Based on directly regenerated plant of in vitro plant of Xinjiang cotton, the cotton epicotyl treated by pre-culture for 3-5 d was immersed in bacteria for 15 min and co-cultured for 2 d. Cephalothin at 100 mg/L was used to inhibit bacteria after 50 mg/L kan selection. And the combination of cytokinin with relatively high content and auxin with low content was used in its directly budding culture to obtain resistant seedlings.

结果]在新疆棉花离体培养植株直接再生植株基础上,3~5d预培养处理过的棉花上胚轴,在浸菌15 min共培养2 d后,50 mg/L kan筛选,用1000 mg/L头孢霉素抑菌,并用相对高含量细胞分裂素和低含量生长素的组合对其进行直接出芽培养,从而获得抗性小苗。

ResultBased on directly regenerated plant of in vitro plant of Xinjiang cotton,the cotton epicotyl treated by pre-culture for 3-5d was immersed in bacteria for 15min and co-cultured for 2d.Cephalothin at 1000mg/L was used to inhibit bacteria after 50mg/L kanselection.And the combination of cytokinin with relatively high content and auxin with lowcontent was used in its directly budding culture to obtain resistant seedlings.

结果在新疆棉花离体培养植株直接再生植株基础上,3~5d预培养处理过的棉花上胚轴,在浸菌15min共培养2d后,50mg/Lkan筛选,用1000mg/L头孢霉素抑菌,并用相对高含量细胞分裂素和低含量生长素的组合对其进行直接出芽培养,从而获得抗性小苗。

Methods A restropective analysis was made of the data of 11 instances undrgoing epigastrium operation review quality analysisResults FDGE usually occured druing 4~10 days postoperatively.11 instances can be cured by nonsurgical;The diagnosis means is main depenies on the gastroscope and gastrointestinal radiography;The rear 9 instances (82%) who received nonoperative therapy restored in fifth weeks postoperatively.2 cases (18%) who recovered afterwards starts the a sick person in half a year.

对1996—2002年临床诊治11例施行上腹部手术后发生术后胃瘫病人进行回顾性分析。结果功能性胃排空障碍发生在术后4~10 d.11例均非手术治疗痊愈;非手术治疗后9例(82%)胃肠动力在术后4周内恢复,2例(18%)在手术半年后恢复。

Objectie: To determine radiographically the anatomic length difference between the nasal and oral aerodigestie tracts and to clarify gaps in the literature regarding standardized landmark measurements and documentation techniques in transnasal esophagoscopy, as opposed to traditional transoral esophagoscopy.

目的:因为反对传统的口食道镜检查,本研究确定放射影像学上呼吸消化道起始端鼻腔和口腔之间的解剖长度差异,并阐明文献报道的关于其间隙标准化的界标测量和鼻食道镜记录技术。

This article provided a new route to produce 4-dimethoxy phenethylamine. In basic condition, the product could be obtained through etherization, bromation, Grignard reaction, hydroxyethylation, esterification and Gabriel reaction with phenol as raw material, the overall yield is 45.0%.

提出了合成4-甲氧基苯乙胺的新方法,即在碱性条件下,苯酚首先与硫酸二甲酯反应,再选用高选择性的溴化剂溴化后,格氏反应、羟乙基化反应和酯化反应,最后Gabriel反应可以制备目标产物,反应总收率为45.0%。

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Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

However, to get a true quote, you will need to provide detailed personal and financial information.

然而,要让一个真正的引用,你需要提供详细的个人和财务信息。