终止链

The association between the G residues requires that two of them change the orientation of the base with regard to the sugar (from the usual anti to the unusual syn configuration).

我们不知道端粒的互补链是如何组装的，但是我们可以假设它用终止G-T发夹的3'-OH作为DNA合成的引物来合成。

When termination occurs partly by disproportionation, the prepolymer chains in the product carry branches as well as cross-links.

当终止反应一部分是由歧化所起时，则在产物中预聚物链不但带有交联也带有支链。

Methods Genomic DNA samples of two isolated Plasmodium falciparum isolate strains prepared directly from 5 cases of cerebral malaria patients′ blood in mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding FC27 merozoite surface protein 2 (MSP2) of Papua New Guinea strain of Plasmodium falciparum were used as primers. The PCR products were digested with BamH1 and Hind Ⅲ respectively, and the generated fragment MSP2 were cloned into M13mp18 and M13mp19 vectors and their DNA was analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应对5例中国脑型疟患者恶性疟原虫云南省勐腊县勐罕分离株和云南省盈江县农场分离株基因组裂殖子表面蛋白2(MSP2)基因进行扩增，将扩增产物分别经BamHI和Hind Ⅲ双酶切后，回收的MSP2基因分子定向克隆M13mp18和M13mp19载体，按Sanger双脱氧链终止法进行DNA序列测定，并与恶性疟原虫株FC27、K1、IC1和CAMP株进行同源性分析比较。

By using PCR method, a 1kb fragment was gained from Heliothis amigera nuclear polyhedrosis virus genome.

用PCR方法从棉铃虫核多角体病毒基因组中扩增到了1kb的DNA片段，用双脱氧链终止银染测序法测定了该片段的全序列，发现它含有一个完整。。。

Translation termination is the last step of protein biosynthesis, which contains hydrolysis of peptidyl-tRNA and release of renascent peptide.

翻译终止是蛋白质合成的最后一步，它包括肽酰t-RNA的水解以及新生肽链的释放。

In the forespore itself, another factor, G, is the product of one of the early sporulation genes.

在终止点上，酶停止向生长的RNA链添加新的酶，释放终产物，并且从DNA模板上分离。

The PCR products respectively from a donor (TX2), a chronic hepatitis B patient (TX3), a chronic hepatitis C patient (TX4) and a hepatitis patient with unknown etiology (TX1) were sequenced by the dideoxy chain terminating method.

以套式聚合酶链反应测定TT病毒核酸，取献血员(TX2)、慢性乙型肝炎(TX3)、慢性丙型肝炎(TX4)及慢性非甲～戊型，非庚型肝炎患者(TX1)各1例TT病毒PCR阳性产物，以双脱氧核苷酸链终止法测定核苷酸序列。

The multi-copy expression vector pZM is a shuttle plasmid which can replicate in Escherichia coli, Streptomyces and S. erythraea. It contains PermE promoter, fd terminator, multiple clone site, thiostrepton and ampicillin resistance genes, and ColE1 ori and pJV1 ori replicons which make the vector replicate in E.

游离型多拷贝表达载体pZM是可以在大肠杆菌、链霉菌和糖多孢红霉菌中扩增的穿梭型质粒，带有PermE启动子、fd终止子、多克隆位点、硫链丝菌肽和氨苄青霉素抗性基因以及在大肠杆菌和糖多孢红霉菌中复制的ColE1 ori和pJV1 ori复制子，载体大小9880bp。

Thedideoxynucleotides act as chain terminators since they have no 3'-OHgroup on the deoxyribose which is needed by the synthesis step.

双脱氧核苷酸在脱氧核糖上没有聚合酶延伸链所需要的3'－OH基团，所以可被用作链终止试剂。从得到的PAGE胶上可以读出我们需要的序列。

The dideoxynucleotides act as chain terminators since they have no 3'-OH group on the deoxyribose which is needed by the synthesis step.

双脱氧核苷酸在脱氧核糖上没有聚合酶延伸链所需要的3'－ OH 基团，所以可被用作链终止试剂。从得到的 PAGE 胶上可以读出我们需要的序列。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。