终止链

Objective:To analyse the McAb anti blood group A substance secreted by 50A hybridoma at molecular level and then lay the foundation for preparation of the reagent used for blood grouping of new generations genetic engineering antibody.

从杂交瘤细胞提取总RNA，经RT－PCR扩增、克隆抗体的轻、重链可变区基因，用PCR及双脱氧核苷酸链终止法分析其核苷酸序列，采用PCR技术重叠延伸拼接法构建50A单抗单链抗体基因。

A detailed analysis of Schulz—Flory distribution has been given and a calculation method for growth rate and termination rate of Chain in Fischer Tropsch synthesis is presented here.

经过对Fischer—Tropsch合成产品分布和Schulz—Flory方程的研究与分析，、提出了对决定F—T合成产品分布的重要参数——链生长几率、链生长速率及链终止速率的直接计算方法。

A detailed analysis of Sehulz—Flory distribution has been given and a calculation method for growth rate and termination rate of Chain in Fischer Tropsch synthesis is presented here, Calculation method was based on experiment data and the development doesn't need to be confined by postulating mechanism.

经过对Fischer—Tropsch合成产品分布和schulz—Flory方程的研究与分析，提出了对决定F—T合成产品分布的重要参数——链生长几率、链生长速率及链终止速率的直接计算方法。

It should be noted that the authors consider here only industry value chains terminating with the consumer, as opposed to intermediate goods value chains.

应该指出的是，作者在这里只考虑产业价值链与消费者终止，而不是中间产品的价值链。

Falciparum isolates prepared directly from 5 cases of cerebral malaria patients'blood in Mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding the regions 12—17 in MAD20 merozoite surface protein 1(MSP1) of Papua New Guinea strain of P . falciparum were used as primers. The PCR products were digested with EcoRI and KpnI, respectively, and the generated fragment regions 16—17 were cloned into M13mp18 and M13mp19 vectors and their DNA were analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应技术对中国5 例脑型疟患者恶性疟原虫云南省勐腊县勐罕CMH/YN 分离株和云南省盈江县农场CYJ/YN 分离株基因组DNA 裂殖子表面蛋白1 (MSP1)第13—17 区基因进行扩增，将扩增产物分别经EcoRI 和KpnI 双酶切后，回收的MSP1 第16—17 区基因分子定向克隆M13mp18 和M13mp19 载体，按Sanger 双脱氧链终止法进行DNA 序列测定，并与MAD20、K1 和Wellcome 株原型基因进行同源性分析比较。

Yellow-throated marten\'s relationship is between Stone marten and Fisher marten.Fisher marten is the first species who was divided from Martes.The second one were Yellow-throated marten and Stone marten.Based on the analysis of the fossil note,Martes came from Central Europe,Fisher marten,Yellow-throated marten and Stone marten belonged to a branch which evolved to temperate zone.Boreal forest martens was another branch which evolved to cold environment.It is suggested that Yellow-throated marten and Fisher martenwere ascertained genus separately.4、By contrasting and analyzing 11 complete mitochondrial control region gene sequences of Mustelidae,the extended termination associated sequence domain、the central domain and the conserved sequence block domain were identified.A extended termination associated sequence(ETAS1) and eight conserved sequence(CSB-F、E、D、C、B、1、2、3) was indicated,and sequence mode was presented.Different kind of short repeat sequences were found between CSB1 and CSB2.With wolf as the outgroup,the phylogenetic evolution relationship of Mustelidae was analyzed using the neighbor-joining method.

而基于鼬亚科动物线粒体DNA12种重链编码蛋白基因序列及石貂和渔貂线粒体ND2和cyt b全序列，以狼、狗獾和水獭为外类群，应用NJ、MP和ML法构分别构建貂属和貂熊的系统进化树，结果表明：紫貂与日本貂亲缘关系较美洲貂近，石貂与真貂亚属亲缘关系较近，黄喉貂介于石貂和渔貂之间；渔貂是最早从貂属分化出的种类，其次是黄喉貂和石貂；结合化石记录分析，貂属起源于中欧地区，渔貂、黄喉貂和石貂属于一个向温带进化的分支，而真貂亚属为另外一个向寒冷环境进化的分支，建议将黄喉貂和渔貂分别定为单独的属。4、比对并分析11种鼬科动物线粒体DNA控制区序列，识别出延长终止序列区、中央区和保守序列区3个区域，指出了一个终止相关序列ETAS1及8个保守序列(CSB-F、E、D、C、B、1、2和3)，并给出了序列通式，在CSB1和CSB2之间发现具有不同形式的短串联重复序列。

Furthermore, phosphorylated ACV directly inhibits HIV-1 reverse transcriptase, terminating DNA chain elongation, and can trap RT at the termination site.

此外，磷酸化的阿昔洛韦能直接抑制HIV-1逆转录酶，将其阻止在终止位点，从而终止DNA链的延长。

The end-nodes of the supply chain are always Points of Sales, where demand is entered into the integral system; by POS we do not refer only to consumer outlets, but to terminating nodes of the supply chain that are linked to retailers.

结束节点的供应链都是销售点，其中需求是进入积分系统，通过POS机我们不只是指消费者发售，但终止节点供应链链接到零售商。

For most viruses,there is aneed for antimicrobials that target unique viral molecular properties.Acycloviris one such drug.It is activated into ahuman herpesvirusDNA polymerase inhibitor exclusively by HHV kinases and,thus,does not suppress other viruses.Here,we show that ACV suppresses HIV-1in HHV-coinfected human tissues,but not in HHV-free tissue or cell cultures.However,addition of HHV-6-infected cells renders these cultures sensitive to anti-HIV ACV activity.We hypothesized that such HIV suppression requires ACV phosphorylation by HHV kinases.Indeed,an ACV monophosphorylated prodrug bypasses the HHV requirement for HIV suppression.Furthermore,phosphorylated ACV directly inhibits HIV-1reverse transcriptase,terminating DNA chain elongation,and can trap RT at the termination site.These data suggest that ACV anti-HIV-1activity may contribute to the response of HIV/HHV-coinfected patients to ACV treatment and could guide strategies for the development of new HIV-1RT inhibitors.

对大多数病毒而言，都需要有针对其分子特性的靶向杀毒剂阿昔洛韦就是这样一种靶向药物在人疱疹病毒酶的特定作用下，阿昔洛韦被激活成为人疱疹病毒DNA聚合酶抑制剂，因此不能再抑制其它的病毒我们的研究发现阿昔洛韦在共感染人疱疹病毒的组织中可以抑制HIV-1，但在无人疱疹病毒感染的组织或细胞中无此作用然而，加入人疱疹病毒-6感染的细胞却使得其对抗HIV的阿昔洛韦变得敏感我们推测这种抑制作用依赖于人疱疹病毒酶导致的阿昔洛韦磷酸化实际上，单磷酸化的阿昔洛韦前体药物无需人疱疹病毒的参与即可抑制HIV此外，磷酸化的阿昔洛韦能直接抑制HIV-1逆转录酶，将其阻止在终止位点，从而终止DNA链的延长这些结果提示阿昔洛韦的抗HIV-1活性决定了艾滋病病毒/人疱疹病毒共感染的患者对阿昔洛韦的治疗反应，也有助于开发新的HIV-1逆转录酶抑制剂

The experiments showed that polypeptides were formed by self-assembly of N, O- bis amino acids with the assistance of o-phenylene phosphoro- chloridate. Three steps were involved in the self organization reactions, i.

侧链无官能团的N，O-二-α-氨基酸在O，O-亚苯基磷酰氯辅助下能自组装得到系列2～n聚寡肽，其自组装成肽过程大致分为三步：氨基酸的活化、肽链的延长和链反应终止。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。