细菌计数

RESULTS: We built up two standard curves with the results of UV spectrophotometry and wet weight, to plate count, respectively. The analysis of accuracy rating proved that there was an approximal linear correlation between the two standard curves ranging from 105/mL to 108/mL.

结果： 建立了紫外分光光度计法和湿重法相对于平板计数法的标准曲线，通过准确度分析表明在细菌含量在每毫升105～108之间时，两标准曲线线性关系良好。

Have superconduction of Xi Menzi 1.5T machine of CT of many nuclear magnetic resonance, helix and examine of the division full automatic biochemical analyzer, full automatic corpuscular computation appearance, blood coagulates analyzer of appearance, specific protein, full automatic appearance of appraisal of immune glow appearance, bacterium large and medium-sized equipment of medical treatment instrument.

拥有西门子1.5T超导核磁共振、多排螺旋CT机以及检验科的全自动生化分析仪、全自动血球计数仪、血凝仪、特定蛋白分析仪、全自动免疫发光仪、细菌鉴定仪等大中型医疗仪器设备。

The detection limits were 0.05μg/ml for cadaverine,histamine and spermidine, 0.1μg/ml for tyramine, 0.25μg/ml for spermine. The recoveries rate of the method were 96.6%,101.94%,101.90%,98.65%,107.4% for cadaverine, histamine, tyramine, spermidine and spermine, respectively.

通过在发酵香肠中强化接种弯曲乳杆菌6031和肠膜明串珠菌51-5 D单一和混合菌种，使用高效液相色谱法检测了7种生物胺含量的变化，同时进行乳酸菌、球菌、总细菌、肠杆菌的计数。

With the denaturation and protein refolding, we gained the recombinant PlyC. To determine its biological activity, we adopted turbidimetry and plate count method. Before and after lysin treatment, the cell morphology was studied by scanning electron microscopy.

用透析复性方法得到目的产物重组链球菌噬菌体裂解酶PlyC，以浊度法和平板计数法检测其体外抗菌效果，扫描电子显微镜观察裂解酶作用前后细菌细胞形态变化。

Before and after lysin treatment, the cell morphology was studied by scanning electron microscopy. The results showed that the recombinant PlyC could specifically cleavage Streptococcus pyogenes (group A β-hemolytic streptococci).

用透析复性方法得到目的产物重组链球菌噬菌体裂解酶PlyC，以浊度法和平板计数法检测其体外抗菌效果，扫描电子显微镜观察裂解酶作用前后细菌细胞形态变化。

Measurement of certain metabolic parameters may also be used to quantitate bacterial populations.

一些代谢参数的测量可以用来计数细菌的群体量。

Methods The selected microorganisms were cultivated at 37℃ in culture media with different concentration of sodium arsenite and counted by microscope, and arsenic content in bacteria was detected by ASA.

采用在培养基中加入不同浓度亚砷酸钠的方法研究各菌株对砷的耐受性，并在显微镜下进行细菌量计数，采用超低温捕获原子吸收分光光度仪测定菌体中砷含量。

A basic knowledge of microbiology, will be detected with a microscope observation of micro-organisms, medium Preparation, disinfection and sterilization, separation and purification of micro-organisms, bacteria counting method A chemical analysis of the theoretical knowledge and can use chemical analysis methods and equipment of the components of material analysis Master fermentation process principles, understanding of beer, liquor, fermentation production technology Master protein related knowledge, PAGE gel electrophoresis technology, high-speed centrifugal Genetic engineering master the basic theory of knowledge, the extraction and purification of genes, PCR principle, gene recombination technology Master of the basic theoretical knowledge, the immobilized enzyme, the enzyme preparation Application Master of animal and plant tissue culture techniques of experimental steps studied experimental apparatus: Balance (scales, electronic scales, Analytical Balance), or acidity, spectrophotometer, liquid gas chromatography, acid-base titration apparatus, microscopes, centrifuges, ultrasonic cleaning, constant temperature oscillation incubator, super-clean table, steam sterilization pot, the pot constant temperature water bath, PAGE device apparatus, oven, fermenter, such as PCR instrument.

具备微生物理论基础知识，会用显微镜检测观察微生物，培养基的制备、消毒与灭菌，分离与纯化微生物，细菌的计数法具备分析化学的理论知识，能运用化学分析和仪器分析方法对物质的组分进行分析掌握发酵工艺原理，了解啤酒、白酒、发酵生产工艺掌握蛋白质相关知识，PAGE凝胶电泳技术，高速离心掌握基因工程的基本理论知识，基因的提取和纯化，PCR技术原理，基因重组技术掌握酶的基本理论知识，酶的固定化方法，酶制剂的应用掌握动植物组织培养技术的实验步骤学过的实验仪器：天平（台天平，电子天平，分析天平）、酸度计、分光光度计、液气相色谱仪，酸碱滴定仪器，显微镜，离心机，超声波清洗器、恒温振荡培养箱、超净工作台，蒸气灭菌锅、恒温水浴锅，PAGE电泳装置仪器、干燥箱、发酵罐、PCR扩增仪等。

Samples of bacterium taken from Huguangyan Maar Lake are isolated and purified using spread plate method and streak plate technique. The partial characteristics is analyzed morphologically and physiologically.

采用涂布法、划线分离法以及形态学和生理生化特性分析，对湛江湖光岩微生物进行了分离纯化及部分性质研究；同时用平板菌落计数法对其异养细菌、真菌和弧菌等的数量进行初步统计。

METHODS: The 144 BALB/c male mice were abdominally irradiated with 1150 cGy 60Coγray in 1-3 h, and then they were injected intestinal RNA from normal rats on jejunum. On day 1, 3 and 5 after irradiation,the mice were sacrified after anesthesia for determining endotoxin in blood and bacterial metathetic rate, and intestinal crypt survival rate.

选用BALB/c雄性小鼠144只，用1150 cGy 60Coγ射线进行腹部一次照射，于照后1~3 h采用局部肠腔扩张注入法给小鼠空肠肠腔内注入正常大鼠小肠RNA，分别于照后1，3，5 d麻醉后解剖，取血，测定内毒素含量；取肠系膜淋巴结，测定细菌移位率；取空肠段，计数肠腺存活率。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。