细菌蛋白

The results shows that suppurative hemolytic streptococus such as S. pyogenes, S. agalactiae and S. eguisimilis are obviously related to guttate psoriasis, and M6- proteins plays an important role. Other bacteria containing M6- protein liking S. uberis can alsc induce or exaggerate the psoriasis.

研究结果显示，化脓性溶血链球菌中的化脓链球菌、无乳链球菌和似马链球菌与点滴型银屑病的发生有明显相关性，可能主要是菌体中的M6蛋白在起作用，其它含有M6蛋白的细菌如乳房链球菌也可诱发或加重银屑病的发生。

The PCR based assay was tested on 19 strains isolated in throats from patients with guttate psoriasis. The results demonstrated that 6 strains were positive in 15 streptococus strains among which there were 3 streptococus pyogenes, 1.S.agalactiae, 1 S. eguisimilis and 1 S. uberis, and 4 non- streptococus strains were negative. Emm6 was found not only in suppurative hemolytic streptococus, but also in S.uberis.

应用聚合酶链式反应方法对点滴型银屑病患者咽部培养出的19株细菌中的M6蛋白基因进行检测，结果，15株链球菌中6株为阳性，其中化脓链球菌3株，无乳链球菌、似马链球菌和乳房链球菌各1株，4株非链球菌均为阴性。M6蛋白基因不仅存在化脓性溶血链球菌中，在其它链球菌如乳房链球菌中也存在。

The fatter mice in Gewirtz's study had been bred to lack a protein known as toll-like receptor 5 (TLR5), which most intestinal cells sprout on their surface. Its job is to recognize and bind to the whiplike flagella that bacteria use to move around. TLR5 acts as a traffic cop for controlling the mass of pathogens living in the intestine; without it, the normally harmless gut bacteria tend to overflourish and expand in number. See and listen to an audio slideshow about obesity rehab.

Gewirtz 实验中的胖小鼠普遍缺乏一种被称之为 toll 样受体5 (TLR5)的蛋白，这种蛋白是肠上皮细胞表面分泌的， TLR5的作用是识别并结合在肠道细菌的运动器官鞭毛上，在对大多数肠道病原体的控制中， TLR5扮演着交通警察的角色，没有它，一般无害的肠道菌就会异常活跃并且大量繁殖。

Two relevant sites of enzymatic digestion were added to the mTNF-α by PCR. The mTNF-α was linked to the 3'end of m/〓 in pGEX4T-1 vector. The prokaryotic expression vector pGEX4T-1m/〓-mTNF-α was constructed successfully. After induction and expression by IPTG, the expression of two kinds of fusion protein is 15% and 12% of total bacteria proteins respectively. The anti-HCC bifunctional antibodies m/〓-mTNF-α were identified by electrophoresis after the inclusion bodies were purified, denature, renature, re-purified, digested by thrombin and further purified.

采用PCR的方法在mTNF-α的两端加上所需要的酶切位点，将之连接在m／〓的3'端，构建原核表达载体pGEX4T-1 m／〓-mTNF-α，通过IPTG的诱导表达之后，两种融合蛋白的表达量分别占细菌总蛋白的15％、12％，表达产物经包涵体的纯化→变性→复性→纯化→凝血酶酶切→进一步纯化后，可以得到纯度为电泳纯的m／〓-mTNF-α抗肝癌双功能抗体。

PSRP-3, on the small subunit and unassociated with rRNA, is an evolutionarily conserved chloroplast ribosomal protein It is the higher plant orthologue of protein YCF65 (ycf65 gene product) in cyanobacteria.

其中PSRP-3位于30S小亚基上，不与rRNA连接，是一个进化保守的叶绿体核糖体蛋白，YCF65(ycf65基因产物)是它在光合蓝细菌中的同源蛋白。

The mc2155 carrying pMind-glmU-AS was induced by different concentration of tetracycline, and GlmU protein was detected in the mc2155 carrying pMind-glmU-AS by Western blotting. The results showed that tetracycline of 20ng/ml has obviously inhibited the growth rate of mc2155 carrying pMind-glmU-AS, but the amount of GlmU protein did not change compared to that of uninduced mc2155 carrying pMind-glmU-AS.

glmU反义RNA的诱导表达与GlmU蛋白的检测分别用5 ng/ml、10 ng/ml和20 ng/ml的四环素诱导携带pMind-glmU-AS表达载体的mc~2155菌株表达反义RNA，绘制细菌生长曲线，结果表明20 ng/ml浓度的四环素可抑制mc~2155/pMind-glmU-AS的生长，但Western Blotting分析结果显示经四环素诱导和未诱导的mc~2155/pMind-glmU-AS中，GlmU蛋白表达量无明显的变化。

The eukaryotic translation initiation factor 5A (eIF-5A) is a small acidic protein (18k Da), widely presents in all organisms from archebacteria to mammals but not in eubacteria, with highly conserved sequences. eIF5A is unique in that it is the only protein known to contain the unusual amino acid residue hypusine (hydroxyputrescine-lysine), which is essential for eIF5A function and is formed by post-translational modification of a specific lysine residue.

真核生物翻译起始因子5A(eIF5A)是一个分子量约18 kDa的酸性蛋白，其序列从古细菌到哺乳动物高度保守，并广泛存在于各种真核生物细胞中。eIF5A是迄今为止唯一一种已知含有特殊氨基酸残基hypusine的蛋白质。hypusine是一种蛋白翻译后修饰产物，它的形成对eIF5A的功能完整至关重要，相关的研究结果表明hypsine在控制细胞周期循环的过程当中扮演着一个重要的角色。

Design of peptide inhibitors for furin based on the C-terminal fragment of histone H1.2The mammalian proprotein convertase furin has been found to play an important role in diverse physiological and pathological events,such as the activation of viral glycoproteins and bacterial exotoxins.

基于组蛋白H1-2片段的furin抑制剂的设计改造在哺乳动物的许多生理和病理活动中，前体加工酶furin起到了非常重要的作用，例如对病毒糖蛋白和细菌毒素的激活。

Homology analysis demonstrated that, McChi5 protein, which contained a conserved domain of family 18 glycosyl hydrolyse, had the sequence similar to tabacco class V chitinases, several putative chitinases and chitinase-like proteins of Arabidopsis thiliania , and some chitinases from mammals, insects and bacteria.

同源性分析表明，McChi5蛋白与烟草V类几丁酶、拟南芥的推测几丁酶和类几丁酶蛋白、以及哺乳动物和细菌的一些几丁酶有序列相似性，并具有第18家族糖基水解酶的保守域。

The recombinants expression the fusion protein at a level of about 35% of total cell proteins.

表达的融合蛋白量约占细菌总蛋白的35%。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。