细菌蛋白

H2-uptake phenomenon of the bacterial ferritin is still found in an analogy system of micro-oxygen environment of entire cell Azotobacter vinelandii in vitro, which indicates that the H2-uptake reaction can be carried out by the ferritin within the cell.

此外，细菌铁蛋白在体外模拟棕色固氮菌整体细胞内的微量氧环境体系中仍有氢还原现象，因而推测细菌铁蛋白在该菌体内也能进行吸氢反应。

The bacterial protein that is used in the present invention is bacterial flagella.

用于本发明的细菌蛋白是细菌鞭毛。

They were named respectively as muramidase-released protein; succinate dehydrogenase/fumarate reductase flavoprotein subunit (SDHFp/FRDFP, extracellular); bacterial trigger factor; elongation factor G (EF-G, extracellular); PTS system sorbose subfamily I (PTS/MAN-ⅡAB, cytoplasmic); pyruvate kinase; phosphofructokinase, PK.

经MALDI-TOF-MS鉴定，这8个蛋白分别为溶菌酶释放蛋白、琥珀脱氢酶／延胡索酸还原酶黄素蛋白亚单位(SDHFp/FRDFp)、触发因子、延长因子G、细菌糖磷酸转移酶系统甘露糖特异ⅡAB亚单位、丙酮酸激酶、6-磷酸果糖激酶(6-PFK)、色氨酸－tRNA合成酶。

Result show:the solution,extracted from rat rail tendons,consist mainly of type I collagen;No growth of bacteria in 37℃ thermostatic culture box for 72 hours;Absorbing the height of ultraviolet-spectrophotometry is 230 nm by using flicker photometer examination;Amino acid in type I collagen consist of aminoacetic acid(40 per cent) and proline(14.3 per cent);Under microscope:collagen gel netted constraction consist of original collagen fibril that had been fairly well-distributed and had a quite regular striate lines ;The collagen solution prepared collagen gel matrixes in 37℃ thermostasis for culture cells and also prepared collagen solid film by washing collagen gel with a phosphate solution for plasty surgery materials.

结果：提取液主要为Ⅰ型胶原蛋白；72小时37℃恒温培养箱内培养无细菌生长；紫外分光光度计测定，吸收高峰为230nm；氨基酸成分主要为甘氨酸：占40%，脯氨酸：14.3%。显微镜下见：相互交织的胶原原纤维结构组成的网状结构；纤维均匀，有规则横纹。胶原蛋白液在37℃恒温下，可制成凝胶，用于培养细胞；用磷酸液冲洗胶原蛋白凝胶可成固体片状，制成胶原膜，用作医用材料。

Antibacterial function of body wall was conducted by the following way: microorganism was directly resisted through the change of cuticle, such as incrassation of up-cuticle protuberance and increasing length of micro-floccus in the up-cuticle dissociation surface; exudation function of large granular orthochromatic mucous cells and reticulation mucous cells in the epidermis could be enhanced when the Eisenia fetida was induced by E. coli. Moreover, the mucus that contained compound substance of mucopolysaccharide and protein could make the bactericidal function or dilute the microorganism on the surface of earthworm, which could protect earthworm from invasion of microorganism; the content of neutral mucopolysaccharide of antibacterial function could be increased according with the exudation increment of small granular proteinaceous cells in the epidermis, which could resist bacteria invading into the epidermis of earthworm.

结果表明：蚯蚓的体壁主要是通过以下途径来抗细菌的侵袭：一是通过角质层的变化(如上胶层突起的增厚、上角层游离面中微绒毛的变长等)直接抵抗微生物的入侵；二是增加表皮中大颗粒粘液细胞和网状细胞的分泌功能，分泌出大量含有粘多糖蛋白液体复合物的粘液，在蚯蚓体表对细菌进行稀释或者起到直接杀菌的作用；三是通过增加小颗粒蛋白细胞的分泌作用，在蚯蚓的表皮中分泌出更多的抵抗疾病的中性粘多糖，从而抵抗进入蚯蚓体表内的细菌。

While protamine inhibit or kill bacteria, bacteria are induced a variety of mechanisms to resist it.

在鱼精蛋白抑制细菌的同时，细菌也产生多种机制对抗鱼精蛋白。

To investigate the presence of specific adhesion activity, the mrkD adhesin gene of the established type 3 fimbriae expression system was replaced respectively by each of the fimbrial adhesins. Transmission electron microscopy and western blot analysis revealed that most of the recombinant type 3 fimbriae expressed and assembled properly.

位於纤毛末端的黏附蛋白为决定细菌黏附专一性的主要因子，为了测试黏附蛋白的活性，我们进一步以过去建立的第三型纤毛表现系统为平台，将MrkD黏附蛋白基因分别置换为kpa、kpb、kpc、kpd、kpe、kpf或kpg的黏附蛋白基因，并经西方墨点法确认这些重组第三型纤毛的结构蛋白的表现和组装正常。

①The collected clinical Acinetobacter baumannii isolates were more seriously resistable to Ciprofloxacin than to Imipenem;②The antibiotic resistance of clinical Acinetobacter baumannii isolates may be associated with the decline of CarO protein expression;③There is some relationship between CarO protein and ciprofloxacin-resistant Acinetobacter baumannii strains,it proves that CarO protein supports bacteria on multi-drug resistance;④CarO protein may be related to cell signalling;⑤The difference in CarO protein functional sites and structure between the sensitive strains and the resistant strains may be related to bacterial resistance.

①收集的临床鲍曼不动杆菌对环丙沙星的耐药情况较亚胺培南严重；②CarO蛋白的表达下调可能与临床鲍曼不动杆菌的耐药性相关；③CarO蛋白与鲍曼不动杆菌耐环丙沙星有一定相关性，佐证了CarO蛋白可能与细菌多重耐药有关；④CarO蛋白可能与细胞信号传导有关；⑤敏感菌株与耐药菌株的CarO蛋白功能位点和内部结构有差异，可能与细菌耐药有关。

Results After screening, 59 subtracted library clones were isolated which were specific for strain VIB72, and the DNA sequences of these clones were determined. Seventeen fragments showed high homology to the genes of known functions in other bacteria. This includes soluble lytic murein transglycosylase, mobilization protein, transposase (IS66), resistance-related protein (metallo-beta-lactamase and acetyltransferase family), toxin protein (DT-201 and alveicin A immunity protein), ATP-dependent endonuclease of OLD family like protein, SocE and GTP-binding protein HflX (high frequency of lysogenization).

通过对差减文库筛选，分离到59个对菌株VIB72的克隆，并对这些克隆的DNA序列进行了测定。17个基因片断与其它细菌的已知功能的基因有较高的同源性，其中包括可溶性溶胞壁质转糖基酶、转移蛋白MobA和MobC、转座子IS66、抑制相关蛋白（金属β-内酰胺酶和乙酰转移酶家族）、毒素蛋白（DT-201和alveicin A免疫蛋白）、与OLD 家族相似的ATP依赖性核酸内切酶以及SocE 和GTP结合蛋白HflX。

A bacterial protein conjugated to small antigenic determinants such as peptides or other small haptens.

本发明涉及一种与小的抗原决定簇结合的细菌蛋白，如肽或其它小的半抗原。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。