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Pentosaceus. These four bacteriocins showed the highest antibacterial activity under acidulous and neutral conditions. The bacteriocin-producing strains inhibited most of related lactic acid bacteria. The bacteriocins produced by strains L. acidophilus Ind-1 and P.

戊糖片球菌产生的细菌素对木瓜蛋白酶敏感,而其它三种细菌素对这种蛋白酶稳定。pH稳定性研究表明,在弱酸性或中性条件下,这些细菌素表现出较高的活性。

Summarizing the studies obtained,sediment,commensal,and symbiotic bacteria were more important in searching for nature pharmaceutics.

从报道的研究结果看,沉积物细菌、共栖细菌和共生细菌是海洋微生物天然药物筛选的重要来源。

Carl Sagan has entertainingly described this phenomenon, musing about the destiny of bacteria that reproduce by dividing into two every 15 minutes. Says Sagan:"That means four doublings an hour, and 96 doublings a day. Although a bacterium weighs only about a trillionth of a gram, its descendants, after a day of wild asexual abandon, will collectively weigh as much as a mountain...in two days, more than thesun - and before very long, everything in the universe will be made of bacteria." Not to worry, says Sagan: Some obstacle always impedes this kind of exponential growth. The bugs run out of food, or they poison each other, or they are shy about reproducing in public.

Carl Sagan 曾经开玩笑地解释这种现象,假设有一种细菌每15分钟可以分裂一次,亦即代表每一小时复制四次,一天可复制96次,虽然单一个细菌其重量只有一兆分之一公克重,但是只要经过一天无止尽的无性生殖,其重量可能会比一座山还重,经过两天后,甚至会比太阳还重,要不了多久所有的宇宙都将由这种细菌所组成,不过 Sagan 又说,大家不必担心,上天一定会安排障碍来阻止其倍数成长,有可能他会耗尽食物,或是毒死自己,或是有可能羞于在大庭广众之下复制。

Carl Sagan has entertainingly described this phenomenon, musing about the destiny of bacteria that reproduce by dividing into two every 15 minutes. Says Sagan:"That means four doublings an hour, and 96 doublings a day. Although a bacterium weighs only about a trillionth of a gram, its descendants, after a day of wild asexual abandon, will collectively weigh as much as a mountain...in two days, more than the sun - and before very long, everything in the universe will be made of bacteria." Not to worry, says Sagan: Some obstacle always impedes this kind of exponential growth.

Carl Sagan 曾经开玩笑地解释这种现象,假设有一种细菌每 15 分钟可以分裂一次,亦即代表每一小时复制四次,一天可复制 96 次,虽然单一个细菌其重量只有一兆分之一公克重,但是只要经过一天无止尽的无性生殖,其重量可能会比一座山还重,经过两天后,甚至会比太阳还重,要不了多久所有的宇宙都将由这种细菌所组成,不过 Sagan 又说,大家不必担心,上天一定会安排障碍来阻止其倍数成长,有可能他会耗尽食物,或是毒死自己,或是有可能羞于在大庭广众之下复制。

A great deal of bacteria was found intracellularly and extracellularly in these positive samples under transimission electron microscopy. 4. The nanobacteria which was identified by transimission electron microscopy was isolated from 5 atherosclerotic plaque samples.

阳性标本透射电镜观察见细胞间质和细胞浆内均有较多的细菌,细菌表面有一层致密的外衣。4.6例腹主动脉瘤粥样斑块标本中有5例分离培养出纳米细菌,阳性率为83.3%,并予以透射电镜鉴定。6。

The behaviour of bacteria on the surface of grains and in the solution as well as the effect of mineralogical composition and structure on the activity of bacteria were investigated.

细菌氧化动力学研究表明,在本实验范围内毒砂的细菌氧化是以化学反应为主、兼有细菌作用的复杂多相反应过程。

A clone from each genotype was randomly selected as representative for sequencing. The obtained 16S rDNA gene sequences had a similarity of 87%-100% with those in the GenBank (www. ncbi. nlm. nih. gov), and more than half of them had a similarity lower than 97%, being of new species. Based on phylogenetic analysis, the bacteria in the two soils were classified into 10 groups, with 5 groups in common. The dominant bacterial groups in the two soils differed obviously. In primeval forest soil, the dominant group was Proteobacteria, which had 39 genotypes, occupying 58.0% of all the clones; while in the soil of degraded ecosystem the dominant groups were Acidobacteria and Proteobacteria, which had 19 and 15 genotypes occupying 32.5% and 30.5% of all the clones, respectively. In the soil of degraded ecosystem, Proteobacteria group decreased while Acidobacteria group increased markedly, compared with those in primeval forest soil.

从每种基因型中随机选择一个克隆子作为代表进行测序分析,所有序列与GenBank数据库中序列的同源性为87%~100%,且两样地中均有超过一半的基因型序列与数据库中已知序列同源性低于97%,属于分类在"种"地位上的新发现细菌;通过系统发育研究将两样地的细菌分为10大类群,两样地共同拥有5大类群,但两样地的细菌优势类群明显不同,原生土壤为Proteobacteria,含39种基因型,占总克隆子数的58.0%,退化生态系统土壤为Acidobacteria和Proteobacteria,分别含19种和15种基因型,占总克隆子数的32.5%和30.5%;与原生土壤细菌类群相比,退化生态系统土壤Proteobacteria类群明显减少,Acidobacteria类群明显增加。

To sum up, the research for the first time ascertained INA species in apricot trees and their activities by using the rational method to imatate the temperature dropping process in frost nights--computer temperature controlling with semiconductor cooling and artificial frost box with self-recording. Studies were given to responds of INA to freezing temperature of floral organs, biochemical cold resistance parameters, contents of ABA and IAA, chlorophyll fluorescent parameters of young fruits and tissue structure and ultrastructure of apricot. The mechnism of INA induced frost injury of apricot was found out. And it was demonstrated that INA is a critical factor in the inducing and aggravation frost injury of apricot floral organs.

综上所述,本研究采用能够准确模拟自然界霜夜降温过程的较为理想的方法——半导体致冷电脑控温和自动记录的人工霜箱,首次查明了10个杏树品种上INA细菌种类和冰核活性,从INA细菌对杏树花器官过冷却点、抗寒生理生化指标、激素ABA、IAA含量、幼果叶绿素荧光参数以及组织结构和超微结构的影响等方面进行了系统深入的研究,阐明了INA细菌诱发杏树霜冻害的机理,证明了INA细菌是诱发和加重杏花霜冻害的重要因素。

Seven major bacterial isolates were obtained and purified. Their 16S rRNA genes were amplified by PCR for sequencing and their identities were determined with homology comparisons. Five of the seven isolates belong to Actinomycetales including Kocuria KD139, Rhodococcus KD140, Rhodococcus KD142, Arthrobacter KD230, and Arthrobacter KD232; one is classified as Bacillus d KD178; and another one as Stenotrophomonas KD237. The phylogenetic tree was also constructed based on the analysis of 16S rRNA gene sequences.

通过对分离菌株的16S rRNA基因序列进行分析,发现其中5株细菌分别属于放线菌目的考克氏菌属(KD139)、红球菌属(KD140和KD142)和节杆菌属(KD230和KD232), 1株细菌属于杆菌目的芽胞杆菌d属(KD178),另外1株细菌属于黄色单孢菌目的寡食单胞菌属(KD237);同时我们构建了系统进化树,确定分离菌株的相对进化地位。

In this paper, 1554 strains of bacteria were isolated from the soybean rhizophere in the different continuous systems. Those biocontrol bacteria were screened in vitro and in vivo. 6 strains of them had the better control efficacy. They exhibited the different antagonism to the pathogens of soybean root in vitro. The strains 18BR2-2 and 18BR9-1 showed a wide range of antagonism against Fusarium oxysporum, F. avenaoeum, F. solani and Rhizoctonia solani in vitro. The 6 strains exhibited different control efficacy to soybean root rot in greenhouse and in field. In greenhouse, they could decrease the degree of soybean disease significantly, and the control efficacy of 18BR2-2 was the best and can reach 45.90%. In theory, soybean treated by 18BR2-2 may postpone the days of outbreaking of disease for 6 days. The strain 18BR2-2 also promoted the growth of soybean seedling and, raising the soybean height for 11.24%, increasing dry weight for 40.91% and fresh weight for 35.27%.

本研究从长期连作田种植的大豆及其根际土壤中分离细菌1554株,采用平板测试、温室盆栽及田间试验等方法,筛选了对大豆根腐病菌(主要是尖孢镰孢菌Fusarium oxysporum)有很好作用效果的生防细菌6株,在离体的条件下均表现出不同程度的拮抗作用,其中生防细菌18BR2-2和18BR9-1表现出很广的杀菌谱,对大豆根腐病菌尖孢镰孢菌(F.oxysporum)、燕麦镰孢菌(F.avenaoeum)、茄腐镰孢菌和立枯丝核菌都有很好的离体拮抗作用,并且这6株生防细菌在盆栽和田间的情况下均表现出对大豆根腐病一定的防治效果。

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