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Using Pseudomonas syringae and Pantoea ananas , the dominant ice nucleation active bacteria strains from Yunnan province, as receptors of genetic engineering recombination, and Escherichia coli S17/pZJ25∶∶Tn5 as donator. By transposon Tn5 induced mutants method, the Tn5 was inserted and integrated into the genome of wild type INA + bacteria. INA + mutant as INA - when the linear continuous of INA + gene destroyed.

以云南省冰核细菌的优势种类丁香假单胞菌Pseudomonassyringae和菠萝泛菌Pantoeaananas为重组基因工程菌的受体菌,采用Tn5转座子诱变技术,以大肠杆菌EscherichiacoliS17/pZJ2 5∶∶Tn5作为转座子诱变的供体菌,使Tn5插入并整合到冰核细菌基因组中,使冰核基因的线性连续性被破坏,失去冰核活性。

Some antibiotics work by gumming up the ribosomes of bacteria, allowing those bacteria to be stopped at no danger to their host.

有些工作脱胶了抗生素的细菌的核糖体,让这些细菌必须在不停止其主机的危险。

Phylogenetically, they are mainly affiliated to Alpha and Beta-proteobacteria, and some Gamma-proteobacteria-like AAPB also were found newly.

AAPB丰度的季节差异要比异养细菌的小得多,并且AAPB在总异养细菌中的比例高值出现在冬季。

As the aging temperature increases they evolve into rodlike and keep certain orientation relationship with the ferrite matrix.

在抗菌不锈钢抗菌过程的前期,抗菌性能表现为对细菌生长和繁殖的抑制,后期表现为对细菌的杀灭。

This inhibits the ability of the bacteria to grow.

这就阻止了细菌的生长,细菌的数量会逐步减少,皮肤被感染的部分就更容易恢复。

Young therefore suggests that many nanobacteria in the blood may be coated in albumin, and the test detected these.

约翰·杨由此认为血液中的许多纳米细菌的表面可能也包有一层清蛋白。该项试验也的确检测到了一些这样的纳米细菌。

Gerba is the germ guy. He has spent years swiping cotton swabs across the floors of bathrooms, counters, tables and door handles, trying to figure out where the ickiest of germs live.

Gerba一直在研究细菌,他曾花费好几年的时间来做他的一项研究,既是用棉签擦拭卫生间地板、收银台、桌子、和门把手等以收集标本,看看什么地方最适合细菌的生存。

In vivo-induced genes spd_0300, spd_0414, spd_0622, spd_1663, spd_1719, spd_0235, spd_0873, spd_1672 regulated by transformation were screened out and they may participate in the processes such as growth regulation, temperature reception, carbohydrate metabolism, and lipoids metabolism.

筛选出受转化调控的体内诱导基因spd_0300、spd_0414、spd_0622、spd_1663、spd_1719、spd_0235、spd_0873、spd_1672,它们可能参与生长调节、温度感应、糖脂代谢等环节,表明细菌转化可通过调节某些体内诱导基因的表达来增强细菌的毒力。

Different frequency and density of INA bacteria were showed in the different parts of tea plant.

此外,在雨水较多、湿度较大的季节茶树上冰核细菌的密度较高,而在干燥寒冷的时候则较难分离得到冰核细菌。

The optimized conditions for isolation and culture of endophytic bacteria isolated from grasses in alpine area in this study. The different tissues of grasses were immersed in 0.1% of SDS for 15 min, 3% of NaClO for 3 min, 0.1% of mercuric chloride for 10 min, 75% of alcohol for 1 to 2 min. The endophytic bacteria were isolated after aseptic grinding and diluted to 10^(-3) and using TSA culture medium for 5 to 7 days under 28℃.

通过对高寒牧草内生细菌的分离培养方法研究,得出了优化的分离培养条件,即牧草不同组织器官用0.1%SDS浸泡15min、3%NaClO浸泡3min0.1%升汞浸泡10min、75%酒精1~2min处理后(各步间均用无菌水冲洗3~4次),研磨并稀释至10^(-3),涂布于TSA培养基上,置28℃恒温条件下培养5-7d,可从高寒牧草组织中分离获得数量、种类较多的内生细菌。

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