细菌溶血素

Methods The amount of δ-hemolysin from different strains and growth phases is analyzed by high performance liquid chromatography and the curve of the amount of δ-hemolysin vs growth time was drawn.

采用高效液相色谱法检测不同菌株、不同时段的右溶血素的分泌量，绘制其与生长时间、细菌生长量的关系曲线。

But we have seldom found the reports about it in China; So writer summarized some literature more than twenty years; name,classification and family of hemolysin have been written, especially three of representative species:α-toxin, Streptolysin O and HlyA; Nowaday, A relationship between the hemolysin and pathopoiesis of bacterium is obvious.

另外，细菌溶血素的英文命名并不统一，检索时容易造成漏检或误检；通过查阅大量英文文献资料，归纳汇总近20年报道的溶血素研究进程；该文详细列出了细菌溶血素名称、分类以及各溶血素家族列表。并且分别介绍了其中三种典型的穿孔毒素：葡萄球菌α-毒素、链球菌溶血素-O、埃希氏大肠杆菌溶血素HlyA。

The major direction of the research within the first year will be outlined as following:( 1 )The background study, collection, analysis, and identification of the possible metabolites such as protease, hemolysin, hemagglutinin, cytotoxin, leukocidin, and lipopolysaccharide;( 2 )The collection of the pathogenic factor such as outer membrane protein of vibrio spp.;( 3 )The analysis of pathogenic factor through the application of SDS-PAGE technique; and ( 4 )The activity assay of outer membrane protein on substrate to characterize the possible function or study the reaction mechanism.

致病因子之收集：将利用生物化学之方法，以弧菌属细菌外膜蛋白质为目标，收集与溶血素相关之致病因子。3。致病因子之分析：将利用SDS-PAGE电泳，对所收集得到之致病因子进行分析，并估算其可能之分子量。4。致病因子之活性测试：将利用所纯化之外膜蛋白质进行对受质活性之测试，以确定其功能或可能之作用机制。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。