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细菌抑制

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Twenty two strains(14 bacteria and 8 actinomyces strains) of antagonistic microorganisms against Fulvia fulva were isolated from vegetable field soil.

从蔬菜地田间土壤中筛选出对番茄叶霉病具拮抗作用的22株菌株,其中14株细菌,8株放线菌。孢子萌发抑制试验表明,细菌X14菌株和放线菌F15菌株的发酵液及其离心后上清液对番茄叶霉病菌孢子萌发抑制率均达100%。

The results showed that PSB and organic fertilizer could promote the growth of actinomyces, heterotroph bacteria, azotobbacter, ammonifying bacteria and nitrobacteria significantly, and restrain the growth of denitrifying bacteria in the soils.

结果表明,光合细菌和有机肥显著促进土壤中放线菌、异养细菌、固氮菌、氨化细菌和硝化细菌的生长,而抑制反硝化细菌数量。

The ferment product of strains JF and Al with a broad spectrum of antimicrobial activity, against one or more sensitive microbes, such as Staphylococcus epidermidis, Enterobacter aerogenes, Escherichia coli and Typhoid bacillus etc, were screened out using the filter paper assay, strain C6 have inhibiting effects on VSMC proliferation.

结果:从槲寄生中分离到JF、A1、G6等11株内生真菌;G6对牛主动脉平滑肌细胞增殖有抑制作用;JF、A1发酵产物具有较广语的抗菌活性,对革兰氏阳性细菌如表皮葡萄球菌,革兰氏阴性细菌如产气肠杆菌、大肠杆菌、伤寒杆菌等均具有较强的抑制作用。

We also studied the effect of several solutions for producing zoosporangium in Artificial culture condition, the result shows the hyphea culture with 0.1% KNO3 produced the most quantity of zoosporangiums.

将这8株内生细菌与烟草黑胫病菌共培,进一步测定拮抗效果结果显示YN-55、NN-3、YN-4和YN-10 4株拮抗效果最好,均在66%以上,并且这4种细菌均对孢子囊的产生有较强的抑制作用,而菌株发酵液对孢子囊的产生的抑制作用不明显。

By comparing with the curves of classical antibiotics in Doskochilova system, the antibiotic type of fermentation broths was identified. The antimicrobial tests showed, the inhibition rate of alt the twenty strains were considerably and ten of them reached 80% when the fermentation broths was diluted by 10 times. It showed strongest inhibition effect against Erwinia carotorora (17.75 mm). Organize method tests showed that inhibition rate of fermentation broths of the strain HJ1-2 to Sclerotinia sclerotiorum and Botrytis cinerea was 100% and 71.39%, respectively.

发酵液的抑菌活性测定结果表明,放线菌HJ1-2菌株发酵液对20种供试病原真菌均具有不同程度抑制作用,其10倍稀释液对其中10种病原真菌菌丝生长的抑制率达到80%以上;供试7种细菌中放线菌HJ1-2菌株发酵液对白菜软腐病菌的抑制作用最强,抑菌圈直径为17.75mn;放线菌HJ1-2菌株发酵液对油菜菌核病和番茄灰霉病的抑制率分别为100和71.39%。

By inoculated to tobacco plants, six strains could be re-isolated from the inoculated plants, indicating that they were endophytic bacteria in tobacco. Culture these eight bacteria and Phytophthora parasitica var. nicotianae together, the result showed four endophytic bacteria YN-55, NN-3, YN-4 and YN-10 have the best antagonistic effects.

将这8株内生细菌与烟草黑胫病菌共培,进一步测定拮抗效果结果显示YN-55、NN-3、YN-4和YN-10 4株拮抗效果最好,均在66%以上,并且这4种细菌均对孢子囊的产生有较强的抑制作用,而菌株发酵液对孢子囊的产生的抑制作用不明显。

According to the correlation of the inhibiting concentration to photobacterium and nitrifying bacterium by nitrification substrates and products, the change of luminous intensity of photobacterium can indicate the inhibition from nitrification substrates and products.

根据硝化基质和产物对发光细菌和硝化细菌抑制浓度的相关性,可用发光细菌发光强度的变化指示硝化基质和产物的抑制作用。

The power-time curves of E.coli reacting with Co-SG at different temperature has been determined by microcalorimtric method The multiplication rate constant k, generation time G,bacterial growth inhibition ratio I,total thermogenetic quantity Q, the heat quantity of a single bacterium Q_(0) and the heat quantity of a single bacterium per minute have also been calculated according to the power-time curves.

用微量热法测定了大肠杆菌及其在Co -SG配合物作用下的热谱曲线,计算了速率常数k ,传代时间G、抑制I、总产热量Q、单个细菌的产热量Q0 和每分钟单个细菌的产热量 Q0 ,建立了部分代谢参数之间的关系,探讨了大肠杆菌在不同温度和Co -SG作用下的代谢抑制,发现可用t、tr、tg和 Q0 定量表征在不同温度下大肠杆菌的生长代谢和药物的抗菌活性。

After insecticide carbofuran was applied to soil, the results were compared with control soil: The number of soil aerobic bacteria actinomyces, ammonifying bacteria and mold was strongly restrained, and the restrainratio and pesticide concentration have positive correlation.

土壤中添加杀虫剂呋喃丹后,与未添加农药的对照土壤相比,土壤中好氧性细菌、放线菌、氨化细菌、霉菌均受到较强的抑制,且抑制率与施药浓度呈正相关性。

In order to improve the efficiency of isolating actinomycetes from soil and solve the problem of contamination by bacteria and fungus when actinomycetes isolated and cultivated, the effect of K2Cr2O7, actidinoe, nystatin and curbenduzim on bacteria, fungus and actinomycetes was studied in this article.

为了提高从土壤中分离放线菌的效率,解决放线菌分离培养中细菌和真菌的污染问题,研究了重铬酸钾、放线菌酮、制霉素和多菌灵等4种抑制剂对细菌、真菌及放线菌的作用,在此基础上提出了用重铬酸钾作为高效、方便、廉价的放线菌杂菌抑制剂的新观点。

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