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细菌抑制

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The columna soil study showed that strain NBT had the ablity of potassiumuptake ,making losed potassium from the soil inoculated with the bacterium wasdecreased by

通过土柱试验发现,硅酸盐细菌NBT菌株还具有保钾功能,供试土壤接菌处理后的土壤滤液中流失的钾比对照减少35.5-56.5%,经方差分析,差异达显著水平;另外,硅酸盐细菌NBT菌株与农药的相容性试验表明,无论在大田农药施用浓度和残留浓度内的丁草胺、绿黄隆、三唑酮粉、多菌灵、马拉硫磷等农药对NBT菌株没有抑制作用;乐果、甲基1605在大田施用浓度下,对NBT菌株有一定抑制作用,而在残留浓度下,对NBT菌株无明显抑制作用。

The way of high frequency intermittent aeration suppresses the activity of nitrobacteria, and also...

实验结果表明,高频间歇式曝气方式在抑制了硝酸细菌的活性的同时也消除了亚硝酸盐浓度和pH大幅波动对亚硝酸细菌和厌氧氨氧化细菌活性的影响;在曝气阶段,溶解氧浓度被控制在1.2~1.4mg·L-1,亚硝酸细菌成为主体细菌,亚硝酸盐积累;在缺氧阶段,随着溶解氧浓度迅速降低,厌氧氨氧化细菌成为优势菌种,曝气阶段积累的亚硝酸盐与氨氮同时被去除。

S extract had an inhibition effect against Sphaerotheca fuligenea,The inhibition rate was 100% when extract concentration was 20mg/mL and the inhibition rate got to 67.66% even at the concentration of 2.5mg/mL. The extract of E. humifusa had different preventive effects against four strains of S. fuliginea from four districts in Shanghai. The preventive effects on the Qibao and Baoshan were better than those of Nanhui and Qingpu. The microorganism inhibition experiment by filter papers indicated that the extract of E. humifusa had higher inhibition effect against bacteria than on pathogenic fungus. Diameters of the zone of inhibition of salmonellas, Echerichia coli and Staphylococcus aureus were 15.77mm, 12.27mm and 10.53mm respectively. The extract of E. humifusa had a certain effect against Colletotrichum lagenarium, Botrytis cinerea, Rhizoctomia solan and Pyricularia oryza but no effect against Helminthosporium maydis and Gibberella zea. The 4 cation exchange resins and ethanol deposition were used to isolate the active fractions from supernate after alkali deposition.

提取液对黄瓜白粉病菌体外分生孢子萌发抑制实验表明地锦提取液对黄瓜白粉病菌分生孢子的萌发有很好的抑制作用,抑制率随着地锦提取液浓度的增大而增高,当浓度为20mg/mL时,抑制率达到100%,即使在浓度为2.5mg/mL时,抑制率也达到了67.66%;室内盆栽苗期预防性实验表明了地锦提取液对上海4个不同地区的黄瓜白粉病菌均具有不同程度的抑制效果,对七宝和宝山地区的菌株抑制效果优于南汇和青浦地区的菌株;用滤纸片法测定了地锦提取液的抑菌谱,实验结果表明地锦提取液对供试的4种植物病原真菌:黄瓜炭疽病菌、黄瓜灰霉病菌、水稻纹枯病菌和水稻稻瘟病菌均具有一定的抑制效果,而对玉米小斑病菌和小麦赤霉病菌没有抑制效果,对供试的3种人类致病细菌沙门氏菌、大肠杆菌和金黄色葡萄球菌均具有很好抑菌效果,抑菌圈直径分别为:15.77mm, 12.27mm和10.53mm。

The fungicidal activity of methanol extract was higher than that of ethylacetate extract. The bioassay showed that the methanol extracts of strain As fermentation products had certain degree fungicidical activity against Botrytis cinerea, Alternaria longipes, Glomerella cingulata, Curvulavia lunata, Gibberella zeae, Valsa malt under the concentration of 4000μg/mL, the inhibiting ratio against pathogen growth was ranging from 62.9%~85.1%; The results of the inhibition of spore germination indicated that the fermentation products exhibited obvious inhibition rate against Alternaria longipes, the EC50 values was 62.5328μg/mL; The bioassay showed that the methanol extracts of strain As fermentation products had certain degree fungicidical activity against Bacillus cereus , Bacillus subtilis.

其中甲醇提取物的抑菌活性较乙酸乙酯好,在4000μg/mL浓度下对番茄灰霉病菌、烟草赤星病菌、苹果炭疽病菌、玉米弯孢叫斑病菌、小麦赤霉病菌、苹果腐烂病菌6种植物病原真菌均有抑制作用,抑制率在62.9%~85.1%;抑制孢子萌发试验表明:菌丝体甲醇提取物对烟草赤星病菌的毒力较好,其EC50仅为62.5328μg/mL;抑制细菌活性表明菌丝体甲醇提取物对蜡状芽孢杆菌和枯草芽孢杆菌有一定的抑制作用,而对大肠杆菌无明显的抑制作用。

The purpose of this study is to screen microorganisms with growth inhibition against plant fungal pathogens. The plate-dilution method was used to isolate the bacteria from salt marshes at Dafeng, Jiangsu. The antifungal activity of the bacterial broth filtrates against 5 phytopathogens was determined using mycelial disk method. The antifungal compositions of four active bacteria were analyzed primarily with chromatography techniques. Two hundred and thirty-five aerobic (92) and anaerobic (143) bacterial strains have been isolated from the coast salt marsh and sixty-five strains, of which 39 are aerobic and 26 are anaerobic showed greater than 30% of inhibition rate against the growth of at least one of the phytopathogenic fungi on petri dish.

为了开发对植物病原真菌生长有抑制作用的药源微生物,本实验采用平板稀释法从江苏大丰盐沼湿地土壤中分离微生物,并通过菌丝块法检测这些微生物代谢产物对5种植物病原真菌生长的抑制活性,进而利用柱层析分析其活性成分;结果共分离得到235株细菌,其中好氧细菌92株,厌氧细菌143株;体外活性测试结果显示有65株至少对1种测试植物病原真菌的抑制率在30%以上,其中好氧菌39株,厌氧菌26株。

Results The compositions of 28 Chinese herbs can inhibit the growth of 5 bacteria in different levels, but the effects on other 3 bacteria are not obvious. Quercetin and usnic acid have better effects on 5 bacteria than other 26 herbs compositions, with MIC 0.03125~0.125mg/ml and 0.0156~0.0625mg/ml.

结果 28种中药有效成分对3种牙周细菌抑制作用不明显,对另5种口腔常见细菌有一定的抑制作用,其中松萝酸和槲皮素有较强的抑菌作用,MIC值分别在0.0156~0.0625mg/ml和0.3125~0.125mg/ml之间。

objective in order to exploit the natural resources of chinese herbs,the inhibitive effects of the compositions of28chinese herbs on the growth of8common bacteria in oral cavity were determined.methods sen-sitive test of these bacteria were carried out by mtt method.at the same time,chessboard dilution method was used for combined sensitive test.results the compositions of28chinese herbs can inhibit the growth of5bacteria in dif-ferent levels,but the effects on other3bacteria are not obvious.quercetin and usnic acid have better effects on5bacteria than other26herbs compositions,with mic0.03125~0.125mg/ml and0.0156~0.0625mg/ml.conclu-sion quercetin and usnic acid can inhibit the5bacteria growth more effectively than other26herbs compositions.

目的 测试28种中药有效成分抑制8种口腔常见致病菌的作用,为抗菌中药有效成分用于口腔感染性疾病的防治做一些探讨。方法选择28种有抗菌作用的中药的有效成分,采用噻唑蓝法对8种口腔常见致病菌作药物敏感实验,同时采用液体棋盘稀释法进行联合药敏实验。结果 28种中药有效成分对3种牙周细菌抑制作用不明显,对另5种口腔常见细菌有一定的抑制作用,其中松萝酸和槲皮素有较强的抑菌作用,mic值分别在0.0156~0.0625mg/ml和0.3125~0.125mg/ml之间。结论所选28种中药成份中,松萝酸和槲皮素具有较强的抑菌作用。

Pharmacology function】Huang An the AN oxygen Mi Ding be in the Huang An medicine to suppress the germ a very strong kind of the function to synthesize anti-virus medicine, because of it with for germ to synthesize folic acid demand of to ammonia Ji the benzene be sour to have likeness on the structure, exchange with PABA to contend for two hydrogen folic acids to synthesize Mao, bar the germ synthesize two hydrogen folic acids to synthesize Mao, bar the germ synthesize two hydrogen folic acids, end influence nucleic acid protein of synthesize, repress thus germ of growth breed, to variety E-and E+very strongly repress a function.

磺胺间甲氧嘧啶是磺胺类药物中抑菌作用极强的一种合成抗菌药物,因其与细菌合成叶酸时所需要的对氨基苯酸在结构上具有相似性,与PABA兑争二氢叶酸合成酶,阻碍细菌合成二氢叶酸合成酶,阻碍细菌合成二氢叶酸,最终影响核酸蛋白质的合成,从而抑制细菌的生长繁殖,对多种E-及E+有很强的抑制作用。

The results of inhibitor addition to methanogens and to eubacteria showed that both groups of anaerobes especially methanogens played important role in the process of dechlorination.

加入甲烷菌抑制剂氯仿、真细菌抑制剂青霉素降低了PCP脱氯速率,表明甲烷菌对还原脱氯有重要影响,其它厌氧微生物也有不同程度的影响。

Objective To compare two methods of phenylketonuria screening for newborn.

目的 比较筛查新生儿苯丙酮尿症的2种检测苯丙氨酸的方法―细菌抑制法和荧光法。

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