细胞遗传学

The principle fields of the Institute's activity are related to molecular genetics, cytogenetics, immunogenetics, embryology, biochemistry, the physiology of animal stress and ethology, embryo biotechnology and biotechnics, introductions of the results of basic investigations into animal improvement programmes, determination of animal genotypes and their adaptation to environmental conditions and production demands.

该所的主要活动领域是关于分子遗传学、细胞遗传学、免疫遗传学、胚胎学、生物化学、动物胁迫和动物行为方面的生理学、胚胎生物技术和应用生物学、改善动物的项目基础研究结果的介绍、动物基因型的决定及其对环境条件的适应与产品要求等方面的内容。

Methods Morphology observation, chromosome cytogenetics, tumorigenesis in nude mice, and the sensitivity to virus were examined.

采用细胞形态学观察、细胞遗传学染色体分析、细胞在裸鼠体内恶性生长情况的组织病理学检测以及细胞对病毒的敏感性等方法。

Florescence in situ hybridization, one of the most important molecular cytogenetic techniques, overcomes the main inconvenience in classical cytogenetic methods in solid tumors.

荧光原位杂交是当前分子细胞遗传学最重要的技术之一，克服了实体瘤经典细胞遗传学研究的主要障碍。

The journal publishes original research articles, research reports, technical notes, scientific commentaries, news, views and review articles on genetics, evolution and molecular biology, including animal genetics, ethics, genetics of microorganisms, plant genetics, human and medical genetics, population and quantitative genetics, mutation and mutagensis, bioinformatics and evolutionary genomics, cytogenetics, and immunogenetics.

杂志刊载原始研究文章，研究报告，技术札记，科学评注，新闻，观点和评论文章，内容涉及遗传学，进化和分子生物学，包括动物遗传学，道德规范，微生物遗传学，植物遗传学，人类与医学遗传学，种群与数量遗传学，突变与突变发生，生物信息学和进化基因组学，细胞遗传学，和免疫基因组学。

To identify whether our previous founding were exist in the series of our newly established cell cultures with the same genetic background and different metastatic potentials, and to make clear what are congenerous and various traits in the chromosomal or DMA sequence levels between these cells, in this study, a combination of conventional G-banding, comparative genomic hybridization, multiplex fluorescence in situ hybridization and arm or locus-specific fluorescence in situ hybridization were used to comprehensively characterize molecular cytogenetics aberrations of the above cell cultures.

为查明我所新建立的这些具有同一遗传背景而又在肝癌的转移潜能上存在明显差异的细胞是否也具有8p的缺失，并弄清它们在其它染色体及其区段的DNA水平上有着怎样的相似和不同，这些相似与不同又有着怎样的意义，本课题联合应用G显带技术、比较基因组杂交技术、多重荧光原位杂交技术和位点或臂特异荧光原位杂交技术，对上述细胞进行细胞及分子遗传学研究，以期了解其分子细胞遗传学异常的特征、找出转移相关的遗传学标记，为探讨肝癌转移机制提供材料。

Different cytogenetical subtypes had different clinical, hematological and cytogenetical characteristics in subacute myeloid leukemia.

不同的细胞遗传学亚型有不同的临床、血液学和细胞遗传学特征。

Methods Totally 173 patients diagnosed as MDS according to FAB or WHO criteria with complete clinical and cytogenetical data were included in this reseach.

方法应用骨髓细胞短期培养法和染色体G显带技术，部分病例联合应用荧光原位杂交技术，对42例细胞遗传学检查具有+8异常克隆，16例具有-7/7q-异常克隆，以及55例虽然经常规细胞遗传学检查未检出异常克隆，但是骨髓涂片原始细胞≥0.10，按照FAB或WHO标准既往诊断为MDS的病例进行了临床及血液形态学和细胞遗传学的系列研究。

Methods Totally 173 patients diagnosed as MDS according to FAB or WHO criteria with complete clinical and cytogenetical data were included in this reseach. Among them 42 had +8 chromosome aberration, 16 had -7/7q-, and 55 had normal karyotypes and more than 0.10 blast cells in the bone marrow. Short term culture and G-banding techniques and in some specimens fluorescence in situ hybridization method were used to do chromosome analysis.

方法应用骨髓细胞短期培养法和染色体G显带技术，部分病例联合应用荧光原位杂交技术，对42例细胞遗传学检查具有+8异常克隆，16例具有-7/7q-异常克隆，以及55例虽然经常规细胞遗传学检查未检出异常克隆，但是骨髓涂片原始细胞≥0.10，按照FAB或WHO标准既往诊断为MDS的病例进行了临床及血液形态学和细胞遗传学的系列研究。

After having been sequenced of the genomes in many species, one of the most advanced studies is to cytogenetically locate the DNA sequences to chromosomes, and construct integrated map combined the genetic map, contig map, chromosome banding pattern map, and cytogenetic map.

多种生物全序测定完成后，本领域最前沿的研究之一是将已测顺序进行细胞遗传学定位，作遗传图，重叠群图，染色体带型图和细胞遗传学图的整合图。

As a new molecular cytological method, it has widely been used in cellular genetics and molecular biology.

作为一种新型的细胞分子遗传学技术，目前已广泛应用于细胞遗传学、分子生物学等领域。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。