细胞质结合

In this dissertation, firstly, the conditions of isolation and purification of specific toxin fractions produced by Exserohilum turcicum has been studied, and a high-efficient method has been established;secondly, the toxins were analysed by UV-Vis spectrophotometry;then the effect of specific toxin on death rate of corn leaves protoplast was studied by FDA as a tinct reagent;finally, using ANS as a probe and SDS-PAGE to study the membrane protein of the corn leaves with Htl gene. In a word, the aim of the research is to search some existent evidences of the toxin binding site on protoplasmic membrane, and provide proofs for nosogenesis of specific toxin in terms of cytology and the molecular biology.

本研究对玉米大斑病菌1号小种毒素的特异性组分进行分离，建立了高效的特异性毒素分离纯化方法，并对其进行了紫外波谱分析；用荧光素双醋酸酯染色法，测定了毒素特异性组分对原生质体死亡率的影响；进而用1-苯胺基-8-萘磺酸荧光探针标记法和SDS-PAGE电泳法对特异性毒素组分与Htl基因玉米细胞互作过程中的质膜蛋白进行了研究，旨在证明Htl基因玉米细胞质膜上存在特异性毒素组分结合蛋白，为从细胞和分子生物学角度阐明毒素的致病机理提供证据。

Logic Mathematics For a universal set, the set of all elements in the lysis of target cells on contact.

植物凝血素：任一种与细胞质膜上的特定碳水化合物结合的植物糖蛋白，在实验室中用来加速淋巴细胞的繁殖和红血细胞的凝结

The location and structures of sex-pheromone-producing gland in female H.insularis were studied by EAG,GC,SEM,and TEM.These studies showed that thegland situate in the intersegmental membrane between the eighth and ninthabdominal segments,and is an eversible abdominal fold;Many plump cones disturbon the surface of the gland.The glandular cells of 2-day old virgin female H.insularis are arranged in one layer,among which the central cells are columnarepithelial cells and flat on two sides.The nucleus is irregular elliptical.There isevident conjugation between cells and the involution is more in the basal membraneof cell.Microvilli are distributed on the cytoplasmic membrane and linked withendocuticle on which there are many layers of chitin,and the outer cuticule is staineddeeper.The cell contains bubbles,mitochondria,glycogen deposits,roughendoplasmic reticulum and smooth endoplasmic reticulum.

结合触角电位、毛细管气相色谱、扫描电镜、透视电镜等技术对小线角木蠹蛾雌蛾腹尖末端不同组织部位提取物的测定分析以及腺体位置和形态结构的观察发现：小线角木蠹蛾性信息素分泌腺位于腹部末端8～9节之间，是一个由节间膜特化而成的上皮结构，为一可外翻的腹褶，腺体表面分布着饱满的锥形体，羽化后2天未交尾的雌蛾腺体细胞呈单层排列，腹面中央由密集的柱形上皮细胞组成，细胞排列向两侧延伸至背部，其形状由柱形逐渐变为扁平形，细胞核为椭圆形，细胞与细胞间有明显的胞连接，细胞基底膜基褶较多，质膜上分布着微绒毛，并与内表皮连接，内表皮上有多层几丁质，外角质层染色较深，细胞质中含有空泡、线粒体、脂质粒、粗面内质网和光面内质网。

The results showed that there were differences among treatments in transformation of high quality gene(Glu\|D 1 5+10) in F 1 grains, and heterozygous type was more frequently appeared in the treatment of irradiation. In the F 2 segregative population, heading date deviated to early\|mature parent in the treatment of reciperocal cross and its combination with irradiation, indicating that cytoplasm and irradiation could promote the heading. In F 3 the expressive frequency of (5+10) gene in the cross was lower than that in reciperocal cross, and irradiation treatment could raise the expressive frequency of(5+10) gene. These results showed varience in different crosses.

结果表明，不同组合处理间F1籽粒优质亚基(Glu D15+1 0 )的转化存在差异，辐射处理的多以杂合型出现。F2 分离群体中，反交及反交结合辐射处理的抽穗期明显倾向于早熟的亲本，细胞质与辐射具有促进抽穗期的作用。F3 植株Glu D15+1 0亚基的表达频率，不论是哪种组合，反交时 5+1 0亚基的表达频率高于正交；无论正交还是反交，辐射处理提高了 5+1 0亚基的表达频率，而且在不同组合间存在差异。

As we show in detail in Figure 29.31, the result of Wnt signalling is to increase the level of -catenin, which causes it to translocate to the nucleus.

Ras固定在细胞膜里面向细胞质的一侧，以活化的Ras与Ser/Thr激酶Raf结合，因而将Raf带到质膜，使得Raf被激活。

In unstimulated cells, NF-κB exists in the cytosol with an inhibitor protein termed IκB. Various stimulation lead to the proteolytic degradation of IκB and then NF-κB is released.

在静息细胞中，NF-κB与抑制蛋白IκB结合以非活性的状态存在于细胞质中，各种外界刺激可以导致IκB的降解从而释放NF-κB。

Two types of pyrophosphatase are present in plant tissues: soluble one in cytoplasm and insoluble one on the vacuolar membrane.

植物组织中有两种类型的焦磷酸酶，一类是细胞质中可溶性的无机焦磷酸酶，另一类是与细胞液泡膜结合的不可溶性焦磷酸酶(vacuolar PPase，V-PPase)。

Kelch-like ECH-associated protein-1 (Keap1) normally sequesters Nrf2 in the cytoplasm in association with the actin cytoskeleton, but upon oxidation of cysteine residues Nrf2 dissociates from Keap1, translocates to the nucleus and binds to ARE sequences leading to transcriptional activation of antioxidant and phase II detoxifying genes.

在正常情况下，Kelch样环氧氯丙烷相关蛋白-1 (Kelch-like ECH-associated protein-1， Keap1)可将Nrf2与肌动蛋白细胞骨架一起收集在细胞质内，但是在半胱氨酸残基发生氧化的情况下，Nrf2和Keap1脱离，进入细胞核并与ARE结合，从而激活多种抗氧化基因和II相解毒基因的转录。

The cleaved form of SSH3 translocated from cytosol to nuclear and bound to chromatin.

在细胞凋亡过程中，这种剪切形式的SSH3可以从细胞质转移到细胞核，并结合到染色质。

AtDFA is dihydrofolate synthase localized in mitochondria, it catalyzes one glutamate added to the dihydropteroate and produces dihydrofolat. AtDFB, AtDFC and AtDFD are folylpolyglutamate synthase localized in plastid, mitochondria and cytosol respectively. A short chain ofγ-linked glutamates can be added by FPGS.Folate molecules exist in vivo mainly as polyglutamates and these are preferred by folate-dependent enzymes.

AtDFA为二氢叶酸合成酶(dihydrofolate synthase，DHFS)，定位于线粒体，在它的催化作用下，一个谷氨酸分子与二氢蝶酸(dihydropteroate ， DHP)相结合，形成二氢叶酸；AtDFB、AtDFC、AtDFD为多聚谷氨酸合成酶(folylpolyglutamate synthase，FPGS)，分别定位于质体、线粒体和细胞质，在多聚谷氨酸合成酶的作用下，谷氨酸分子可以逐一连接到四氢叶酸的谷氨酸γ位。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。