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The results showed that there were differences among treatments in transformation of high quality gene(Glu\|D 1 5+10) in F 1 grains, and heterozygous type was more frequently appeared in the treatment of irradiation. In the F 2 segregative population, heading date deviated to early\|mature parent in the treatment of reciperocal cross and its combination with irradiation, indicating that cytoplasm and irradiation could promote the heading. In F 3 the expressive frequency of (5+10) gene in the cross was lower than that in reciperocal cross, and irradiation treatment could raise the expressive frequency of(5+10) gene. These results showed varience in different crosses.

结果表明,不同组合处理间F1籽粒优质亚基(Glu D15+1 0 )的转化存在差异,辐射处理的多以杂合型出现。F2 分离群体中,反交及反交结合辐射处理的抽穗期明显倾向于早熟的亲本,细胞质与辐射具有促进抽穗期的作用。F3 植株Glu D15+1 0亚基的表达频率,不论是哪种组合,反交时 5+1 0亚基的表达频率高于正交;无论正交还是反交,辐射处理提高了 5+1 0亚基的表达频率,而且在不同组合间存在差异。

In response to TCDD exposure, AhR nuclear translocator forms a heterodimer with the aryl hydrocarbon receptor after the ligand-dependent translocation of AhR to the nucleus, and eventually the complex binds to the xenobiotic response elements, which promote transcription of several specific genes, such as CYP1A1.However, the diverse effects of TCDD could not be simply explained by the AhR pathway.

对於戴奥辛的毒性机制,以往的研究认为与芳香基碳氢化合物受体(arylhydrocarbon receptor-AhR)的活化有关;TCDD 活化了AhR,被活化的AhR 从细胞质转移到细胞核内,在细胞核与另一个蛋白质AhR nuclear translocator形成二聚体,并与DNA 上的xenobiotic regulatory element结合,诱发某些基因(如:CYP1A1)的转译与表现;不过,单从AhR的代谢途径并不能解释所有生物上的毒性现象。

objective the aim of this study is to investigate the expression and the distribution of the nerve growth factor during the period of neural tube development of human embryo.method early development of neural tube was studied in human embryos about 35 gestational days by using immunocytochemical abc technique.result there were ngf immuno-positive substances in the cytoplasm and nuclei of neuroepithelial cells in the ventricular zones of neural tube.in the intermediate zone of neural tube,ngf immunoreactivity was detected in the nuclei of some neurons,or the processes of other neurons which contained no ngf-immunoreactive substances in their nuclei;the expression pattern of ngf in the marginal zone of neural tube was similar to that of the intermediate zone.the density of ngf-immunorecative particles was higher on the rostrum side of neural tube than on the caudal side.the ngf immuno-positive cells were also observed among the somites of embryo under the neural tube.conclusion these results suggest that ngf was an important signal molecule to induce neural tube differentiation,and that ngf may play a significant role in regulation of the biological function of neurons in developing neural tube.

目的 研究捷安肽素的抗真菌作用机理。方法采用形态学方法和同位素标记法。显微形态观察经捷安肽素处理后的供试真菌的形态学变化。进一步采用14c同位素标记的特异底物&尿苷二磷酸-(14c)-葡萄糖&示踪,研究捷安肽素对真菌(1,3)-β-d-葡聚糖合成酶活性反应的影响。结果研究神经生长因子在早期人胚神经管发育过程中的定位表达。方法采用免疫细胞化学 abc法染色,研究35天人胚的发育情况。结果在人胚神经管的室管带中,神经元的细胞质和细胞核ngf免疫反应阳性;在中间带,一部分神经元的细胞核ngf免疫反应阳性,另外一部分神经元的细胞核ngf免疫反应阴性,而其突起ngf免疫反应阳性;在边缘带ngf的表达与中间带相似。在神经管的头侧ngf阳性反应较强,神经管的尾侧ngf阳性反应较弱。结论 ngf在人胚神经管免疫反应阳性,表明ngf可能是诱导神经管分化发育的重要信号分子,提示ngf可能在人胚神经管的发育中具有十分重要的作用。神经生长因子;人胚;神经管;发育

Histologically, the seminiferous tubules contained numerous Sertoli cells and more Sertoli-spermatozoa complexes, accompanied by the depletion of Leydig cells with deeply stained nuclei. Mature spermatozoa were stored up in the epididymis, but only a few in the efferent ducts. In the second place was testicular atrophy(32/120; 26.7%). The seminiferous tubules showed moderate to severe inactivity of spermiogenesis with evidence of only spermatogonia, spermatocytes and Sertoli cells. The Leydig cells were obviously decreased in numbers associated with decrease of lipid droplets in their cytoplasms. Testicular hypoplasia was the third disorders(22/120; 18.3%). Only a few spermatogonia and Sertoli cells appeared without any spermiogenesis. The associated changes was decreased in Leydig cells and fibrous hyperplasia in the interstitium.Epididymal stones were sometimes found(12/120; 10%). Grossly, yellowish-white nodules with various sizes and firm in consistency were observed in the epididymis and the front efferent ducts. Microscopically, the epididymal ducts were dilated with voluminous spermatozoa storage, even showed calcification in severe cases. The deposited calcium salts were stained positively by Von Kossa and Alizarin red methods.Amyloidosis was also detected in 10 roosters(8.3%). Eosinophilic, homogeneous, amyloid-like substances were deposited mainly in the testicular interstitium and the periphery of blood vessels. These substances showed positive reaction by Congo red staining. Five roosters(4.2%)had Marek's lesions in the testis, epididymis and peripheral nerves with infiltration of pleomorphic lymphocytes. Only one case showed epithelial necrosis of seminiferous tubules accompanied by fibrous proliferation in the interstitium.

结果发现,在总共搜集的120个病例中,其中因年老所导致的产精力不佳为最多,占38例(31.7%),於镜下可见大量精虫黏附於Sertoli cell的表面,并可见Sertoli cell数量明显增多而Leydig cell明显减少,且其细胞核呈现浓染的现象,而在其副睪中仍可见到成熟精虫蓄留於管腔中,但在其输精管内却只有少量精虫存在;其次为睪丸萎缩,占32例(26.7%),镜下可见中度至重度无造精作用,其生精小管中只见到精母细胞、精原细胞及Sertoli cell存在,但Leydig cell数量明显减少且其细胞质内的脂质也明显减少;睪丸发育不全,占22例(18.3%),於生精小管内只见到精母细胞及少量Sertoli cell存在,不见造精细胞分化,於生精小管间质可见Leydig cell减少并伴随结缔组织增生;副睪结石,占12例(10%),肉眼下可在副睪及输精管前段见到黄白色大小不一的结节,触感坚硬,於镜下可见副睪管扩张并有大量成熟精虫蓄积,严重时可见钙化现象,以Von Kossa及茜素红染色均呈阳性反应;类淀粉沉著症,占10例(8.3%),镜下在睪丸间质及血管周围可见粉红均质样的物质沉积,以刚果红染色成阳性反应;马立克病,占5例(4.2%),镜下可在睪丸、副睪实质及周边神经内均可见到嗜碱性大小不一的淋巴样细胞浸润;睪丸坏死,占1例(0.8%),镜下可见生精小管上皮细胞坏死脱落及间质结缔组织增生。

This page provides some protocols about porcine brain tubulin, sea urchin sperm axonemes, rat liver cell cytosol and organelle fractions, organelle motility assay, tissue culture of PtK1 cells, basic protein chemistry and so on.

该网页提供了一些有关猪脑的微管蛋白、海胆的精子基因丝、大鼠肝脏细胞的细胞质和细胞器的级分、细胞器的运动性实验、PtK1细胞的组织培养、基本的蛋白化学等等方面的操作流程。

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Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

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