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Two polar nuclei move to the micropylar end before sperm is released in a gap between the egg cell and central cell and subsequently taking off cytoplasm one after another. The sperm nucleus moving to the egg nucleus runs faster than the one to the polar nuclei. It fuses with the polar nuclei that are moving towards the antipodal cells at the chalazal end, Images showed the fusion process of male and female nuclei and polyspermous fertilization.

精子释放前,两极核移向卵细胞的合点端;两精子释放于卵细胞与中央细胞的间隙后,先后脱去细胞质,然后分别移向卵核和极核,移向卵核的精核快于移向极核的精核;精核与两极核在向反足细胞团方向移动的过程中完成雌雄核融合。

In this dissertation, firstly, the conditions of isolation and purification of specific toxin fractions produced by Exserohilum turcicum has been studied, and a high-efficient method has been established;secondly, the toxins were analysed by UV-Vis spectrophotometry;then the effect of specific toxin on death rate of corn leaves protoplast was studied by FDA as a tinct reagent;finally, using ANS as a probe and SDS-PAGE to study the membrane protein of the corn leaves with Htl gene. In a word, the aim of the research is to search some existent evidences of the toxin binding site on protoplasmic membrane, and provide proofs for nosogenesis of specific toxin in terms of cytology and the molecular biology.

本研究对玉米大斑病菌1号小种毒素的特异性组分进行分离,建立了高效的特异性毒素分离纯化方法,并对其进行了紫外波谱分析;用荧光素双醋酸酯染色法,测定了毒素特异性组分对原生质体死亡率的影响;进而用1-苯胺基-8-萘磺酸荧光探针标记法和SDS-PAGE电泳法对特异性毒素组分与Htl基因玉米细胞互作过程中的质膜蛋白进行了研究,旨在证明Htl基因玉米细胞质膜上存在特异性毒素组分结合蛋白,为从细胞和分子生物学角度阐明毒素的致病机理提供证据。

Cytopathological study on leaves of pumpkin infected with PRSV-W, WMV showed that there were large amount of inclusion in shape of pinwheel, lamina, and braid in the cells, and there were many linear virus particles fascicled or scattered in the cytoplasm, causing the cytopathological characters which was similar to typical potato virus Y .

对PRSV-W、WMV侵染的南瓜病叶的细胞病理学观察结果表明,在感病细胞中有大量的风轮状、片层状和条带状内含体,线状病毒粒体呈束状或散布在细胞质中,具有典型的马铃薯Y病毒属侵染寄主引起的细胞病理特征。

Cytoplasmic body found in the blood plasma of mammals that promotes blood clotting.

血小板在哺乳动物的血浆中发现的能促进血液凝固的微小的,圆盘状的细胞质个体

A minute,disklike cytoplasmic body found in the blood plasma of mammals that promotes blood clotting .

血小板在哺乳动物的血浆中发现的能促进血液凝固的微小的,圆盘状的细胞质个体

A minute,disklike cytoplasmic body found in the blood plasma of mammals that promotes blood clot ting.

血小板在哺乳动物的血浆中发现的能促进血液凝固的微小的,圆盘状的细胞质个体

Three genes specified expressed in hybrids and one gene over-expressed in hybrid were found. By cloning, sequencing and BLAST analysis, one specific expression cDNA band was cytosolic isocitrate dehydrogenase gene, the other two were new genes which function were unknown, and the over-expressed cDNA band was proteosome subunit p112 gene.

发现了 3 个杂种鸡特异表达的基因和 1 个杂种鸡表达增强的基因,进一步克隆、测序和比对分析初步表明:杂种特异表达的 cDNA 片断分别为鸡细胞质异柠檬酸脱氢酶基因和 2 个未知功能的新基因;杂种表达增强的 cDNA 片断为鸡蛋白酶体亚基 p112 基因。

Inhibition of PKCdelta by rottlerin or knockdown of TG2 protein by a TG2-specific siRNA resulted in a marked increase in autophagy shown by presence of autophagic vacuoles in the cytoplasm, formation of the acidic vesicular organelles, membrane association of microtubule-associated protein 1 light chain 3 (LC3) with autophagosomes, and a marked induction of LC3-II protein, important hallmarks of autophagy, and by electron microscopy.

抑制PKCdelta的rottlerin或击倒的TG2蛋白质的TG2特定的siRNA导致显着增加,表明自噬存在自泡在细胞质,形成酸性囊泡细胞,膜协会微管相关蛋白1轻链3( LC3 )与autophagosomes ,并显着诱导LC3 -Ⅱ蛋白的重要标志吞噬,并通过电子显微镜。

The size of follicle begin primordial follicle to tertiary follicle(1.5~3mm).The number of mitochondrion increased,and the cristae increased.Gogi body change to type,cortical granule increased,and moved behind cytolemma.The proportion of small,middle and large follicles on ovary were 92.51%(92.51±5.87),4.60%(4.60±4.91) and 2.88%(2.88±2.22) respectively.

卵泡在发育过程中,卵泡细胞形成的突起与卵母细胞始终保持密切的联系:卵母细胞从原始卵泡到直径1.5~3mm的三级卵泡,细胞质中线粒体数量由少到多,线粒体内的嵴不断增加,高尔基体由不典型到变成典型,皮质颗粒由开始形成到形成较多的皮质颗粒,并逐渐地迁移到卵母细胞膜下。

Conversion of the different forms of these coenzymes is the main reason for these two enzymes performing the particular function and giving the particular phenotype.

线粒体NADPH的主要供应者Pos5p可以使NAD向NADP转变,细胞质NADPH的主要供应者Zwf1p可以使NADP+向NADPH转变,对不同形式的辅酶的转变是它们行驶特定功能及产生特定表型的重要原因。

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