细胞质基质

Another interesting phenomenon is that when CaLP and p21 are co-transfected, the expression of p21 can stimulate CaLP translocate from cytoplasm to nucleus. By using methods of bioinformatics, point mutation and flow cytometry, we confirmed the importance of Y139 in the nuclear-cytoplasmic trafficking of CaLP.

激光共聚焦实验还发现当p21过表达时能够引起CaLP从细胞质向细胞核的转移，通过生物信息学方法，免疫共沉淀实验和流式细胞分析表明，CaLP的139位酪氨酸残基存在着较高的磷酸化潜力且在CaLP的核质穿梭和蛋白相互作用中起到了重要的作用。

There were three new discovers : 1. The phago-assistant membrane was a nonciliary membrane between two pieces of wave membrane. It tightened with Endoral membrane into cytoplasm formatting cytostome and cytopharynx; 2. Cytoplasm formatted digestive structure which is a stable network digestive channel system but casual food vacuoles. 3. The Oral Primordium field , the assemble and transport of microtubules and membrane were all in the aborve channel system.

有三项新的发现：吞噬辅助膜为位于两波动膜之间的非纤毛膜，并与口内膜一道包卷进入胞质形成胞口、胞咽；细胞质分化出固定的网络状消化腔隙而不是形成临时性的食物泡；口原基场及微管、小膜的装配运输都在此腔隙中进行。

In this dissertation, firstly, the conditions of isolation and purification of specific toxin fractions produced by Exserohilum turcicum has been studied, and a high-efficient method has been established;secondly, the toxins were analysed by UV-Vis spectrophotometry;then the effect of specific toxin on death rate of corn leaves protoplast was studied by FDA as a tinct reagent;finally, using ANS as a probe and SDS-PAGE to study the membrane protein of the corn leaves with Htl gene. In a word, the aim of the research is to search some existent evidences of the toxin binding site on protoplasmic membrane, and provide proofs for nosogenesis of specific toxin in terms of cytology and the molecular biology.

本研究对玉米大斑病菌1号小种毒素的特异性组分进行分离，建立了高效的特异性毒素分离纯化方法，并对其进行了紫外波谱分析；用荧光素双醋酸酯染色法，测定了毒素特异性组分对原生质体死亡率的影响；进而用1-苯胺基-8-萘磺酸荧光探针标记法和SDS-PAGE电泳法对特异性毒素组分与Htl基因玉米细胞互作过程中的质膜蛋白进行了研究，旨在证明Htl基因玉米细胞质膜上存在特异性毒素组分结合蛋白，为从细胞和分子生物学角度阐明毒素的致病机理提供证据。

The mouse embryonic stem cells cDNA library was screened by yeast two-hybrid assay,some candidate molecules were get,including DDX39 DEAD (Asp-Glu-Ala-Asp box polypeptide 39, BAT1A(HLA-B-associated transcript 1A), NAC1 ( nucleus accumbens-1 ), BicD2 (bicaudal D homolog 2)、MLL3 (myeloid/lymphoid or mixed-lineage leukemia 3). NAC1 and MLL3 were associated with regulation of gene transcription. DDX39 andBAT1A were associated with pre-mRNA processing and mRNA export from nucleus to cytoplasm. BicD2 was associated with the regulation of Golgi body.

通过利用酵母双杂交方法，以小鼠全长Plk1作为诱饵蛋白筛选小鼠胚胎干细胞cDNA文库中与其相互作用的蛋白，获得了DDX39DEAD(Asp-Glu-Ala-Aspbox polypeptide 39、BAT1A(HLA-B-associated transcript 1A)、NAC1(nucleus accumbens-1)、BicD2(bicaudal D homolog 2)、MLL3(myeloid／lymphoid or mixed-lineage leukemia 3)等几个候选蛋白，其中NAC1、MLL3与基因的转录调控有关，DDX39、BAT1A与mRNA前体的剪切、加工及mRNA从细胞核转运到细胞质紧密相关，而BicD2与高尔基体的调控紧密相关，提示我们Plk1与基因转录、mRNA前体的剪切、加工、mRNA的运输及胞质分裂中高尔基体的调控有关。

After the first division of the zygote, organelles from male and female gametes appear to be equally incorperated into each cell of the two-celled proembryo.

中央细胞极核周围的细胞质中有各种细胞器，包括质体、线粒体、粗面内质网和高尔基体。

A lot of organelles such as mitochondria, rough endoplasmic reticula and Golgi bodies were observed in fiber cell. Then, double karyotheca disappeared, and the organelles disintegrated. Multivesicular bodies appeared in fiber cytoplasm. With the further development of fiber, fiber wall underwent continual thickening with aging, and polylaminate structure gradually appeared. While the agglutinated nucleus, transfer vesicles, plasma membrane and plasmodesmata still presented.

研究发现，次生壁形成早期，细胞核具有双层核膜，染色质凝聚，可见大量的线粒体、粗面内质网和高尔基体等细胞器存在于纤维细胞中；随后，双层核膜消失，细胞器将逐渐解体，多泡体开始出现在纤维细胞的细胞质；随着年龄的增加，纤维细胞壁逐渐增厚，并出现多层结构现象，而运输小泡、细胞膜、胞间连丝和凝聚的染色质将持续存在。

Microsomes, lysosomes, mitochondria, and cytosol of hepatocytes were isolated by differential centrifugation.

证实正常人肝细胞存在WD蛋白，其存在部分可能在肝细胞质膜、内质网和高尔基器。

Before treatment,there are abundant microvtlli on the cell surface,many filoplodia on the cell rim,few of endoplasmic reticulums , Golgi complexes and mitochondrions with abnormal structure and lots of free ribosomes in the cytoplasm. The shape of nucleus is irregular with high nuclear-cytoplasmic ratio, many dark pellets of heteromatin and a few of nucleoluses in which there ar...

经1.5×10~2g/L地塞米松处理后，细胞表面微绒毛和丝状伪足显著减少或消失，出现皱褶状和小泡状结构；细胞核形规则，核质比值减小，异染色质团块减少，核仁数目减少、结构致密；细胞质中粗糙型内质网、高尔基体、线粒体数量增多，形态结构典型，游离核糖体减少，这些变化说明地塞米松改变了MGc80-3细胞的超微结构特征，具有明显的诱导分化作用。

It is possible to preparing neutralizing MAb which target to active region of Stx2 by synthetical B cell epitope peptides. At present, the crystal structure of the compound of Stx2 and its ligand adenines has been resolved, and the active site of Stx2 is definite, which is the seventy-seventh amino acids, Ganimalon, of Stx2 A1 fragment.

Stx2通过B亚基与受体Gb3结合后，进入细胞，在弗林蛋白酶(一种钙敏感的丝氨酸蛋白酶)作用下A亚基被裂解为A1和A2两个片段，A1片断经内质网进入细胞质暴露毒性活性位点―A1片段发挥N-糖苷酶活性，导致真核细胞蛋白质合成的终止，最终导致细胞的死亡。

Biloba was mainly synthesized in ER and plastids. More or less, mitochondria, Golgi body and ground cytoplasm also took part in the synthesis and transportation of secretory products.

银杏分泌腔内分泌物的合成主要在分泌细胞的质体和内质网中进行，但线粒体、高尔基体和细胞质也似乎参与分泌物的合成和转输。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失