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细胞质

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With the inducing time prolonged, the amount of fusion protein increased, and the highest content amounted to 39. 14% of total protein. Different temperature (15℃, 25℃ and 37℃) could induce expression of fusion protein, and the higher inducing temperature was, the more total fusion protein and soluble fusion protein was produced. Most fusion protein was expressed in the cytoplasm and was soluble, a little of fusion protein was expressed in periplasm or expressed as inclusion bodies, and no fusion protein was found in culture. Furthermore, the results of in vitro enzymatic reaction suggested that the fusion protein could catalyze the methylation of theobromine.

随着诱导时间的延长,表达的融合蛋白产量逐渐增加,表达蛋白总量最高可达到菌体总蛋白的39.14%;在15℃、25℃和37℃三个不同的诱导温度下,均能诱导产生融合蛋白,而且产生的融合蛋白的总量及其可溶性部分所占比例均随诱导温度升高而增加;在菌体的各个部位中,融合蛋白主要在细胞质中以可溶的形式进行表达,有少量在外周质中表达或以包含体形式在细胞质中表达。

In the stage of sporogenous tissue, the Ca^2+ fluorescence intensity was well-proportioned in different cell layer of anther wall.

早期四分体小孢子细胞质中Ca^2+荧光强度较强,胼胝质壁无荧光;后期细胞质中的Ca^2+荧光减弱,而胼胝质壁处Ca^2+荧光增强。

The male sterility resulted from nucleus-cytoplasmic interaction (nuclear genome of common wheat--cytoplasmic genome of D〓-type cytoplasm donor), D〓-type CMS was sporophytic sterility pattern.

一、D〓型CMS系的花粉育性对光周期的变化不敏感(不同于已有光敏不育性的报道),属非光敏细胞质雄性不育系;其不育性起因于核质互作(普通小麦核基因组--D〓型细胞质变异基因组)、属孢子体不育类型;雄性不育受核内隐性基因控制、育性恢复受核内显性基因控制。

The disintegration of tapetal cell was relayed, the inner wall of tapetal cell secreted fewer Ubish bodies.

花粉败育过程中,小孢子液泡膜和细胞质膜断裂破碎,细胞质解体,线粒体、质体、内质网等细胞器解体或退化,绒毡层持续不解体,并缺少乌氏体的分泌。

HOEFERT L L Ultrastructure of developing sieve elements in Thlaspi arvense L.:Maturation 10.2307/2442642 AMERICAN JOURNAL OF BOTANY , 1980,2

王毅%胡适宜棉花小孢子发生过程中细胞质的超微结构变化:着重&细胞质改组&问题植物学报, 1993,4

Methods Telomerase activitiy in cytoplasmic and nuclear extracts of HCC samples was analyzed by an unamplified telomerase assay based on the radioactive incorporation and liquid scintillation counting and silver|staining TRAP(telomeric repeat amplification protocol).

海医系防化医学教研室目的比较肝癌组织细胞质和细胞核中端粒酶活性。方法以端粒酶延伸产物液体闪烁计数法和端粒重复序列扩增法分别检测肝癌组织细胞质与细胞核中端粒酶活性。

By PCR analysis using specific primers, one specific band at 600~700 bp was distinctively present in cms backcrossed generations. It was genetically stable and cytoplasmically inheritable in the various backcrossed progenies.

根据Brassica nap型细胞质雄性不育相关基因orf222设计兼并引物,利用特异引物PCR方法,分别在不育源、F1、BC2以及BC3世代中扩增出一条600~700 bp 大小的特异条带,而在叶用芥菜保持系中无此条带,具细胞质基因遗传特性。

The cytoplasmic male-sterility was transferred into leaf mustard by inter-varietal hybridization and subsequent backcrosses using cms donor of tuber mustard.

采用榨菜细胞质雄性系为不育源,通过变种间杂交和回交的方法,转育叶用芥菜细胞质雄性不育种质。

The cytoplasmic male sterile line CMS96 was transferred into Chinese cabbage by hybridization and subsequent backcrosses using CMS donor of B.napus.

大白菜CMS96细胞质不育系是由大白菜株系与甘蓝型油菜细胞质不育源杂交、多代回交后获得的。

The disorganizing sequence of microspore is as follow: firstly, cell membrane dissolved, cytoplasm degenerated and became thiner; then, nuclear membrane dissolved, nuclear degenerated, and the last was nucleolus.

小孢子细胞解体顺序为:细胞膜首先断裂,细胞质分解变稀薄,然后是核膜断裂,细胞核降解,细胞质及细胞核降解物质充满整个药室,最后核仁解体。

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