细胞质

By using the method of Western blot on fractions of nuclear and cytosol of primary cultured mantle cells and immunogold electron microscopy, we found out the completely different localization of these two proteins: CaM was localized both in cytoplasm and nuclear but CaLP was localized only in the cytoplasm.

通过对体外培养的珍珠贝外套膜上皮细胞核质分离后进行的Western blot分析和免疫电镜研究发现，CaM和CaLP的亚细胞定位是截然不同的：CaM分布于外套膜上皮细胞的细胞核与细胞质中，而CaLP却仅分布于外套膜上皮细胞的细胞质中。

CaLP's phosphorylation state analysis of different localization showed that when CaLP is overexpressed alone, it is in state of phosphorylation and stays in the cytoplasm; when CaLP is co-transfected with p21, p21 overexpression causes its unphosphorylation and translocated from cytoplasm to nucleus.

对定位于不同部位的CaLP的磷酸化状态的分析表明：当在细胞中单独表达CaLP时，它以磷酸化形式存在，且磷酸化的位点发生在Y139上，CaLP因此定位于细胞质中；当共表达CaLP与p21时，p21的过表达引起CaLP发生去磷酸化，CaLP由细胞质穿梭入细胞核并与p21发生直接相互作用。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在囊胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

The smell is usually consists of monoterpenoids produced in Plant Secondary metabolism, and this kind of smell is considered to be Synthesized through two ways-Cytoplasm way and Cytoplast way.

这些气味一般都是属于次生代谢过程中的单格类化合物。产生这些气味目前认为是通过细胞质和细胞质体两种途径来合成的。

In experiments,an injectionchanges the pattern of sinistral eggs,(80) does not influence dextral eggs.

在实验中，用取自右旋卵子的细胞质进行的注射，可改变左旋卵子的模式；用取自左旋卵子的细胞质进行的注射却不能影响到右旋卵子

In experiments, injection of cytoplasm from dextral eggs changes an the pattern of sinistral eggs,but an injection from sinistral eggs does not influence dextral eggs.

在实验中，用取自右旋卵子的细胞质进行的注射，可改变左旋卵子的模式；用取自左旋卵子的细胞质进行的注射却不能影响到右旋卵子。

In experiments, injection of cytoplasm from dextral eggs changes the pattern of an sinistral eggs,but an injection from sinistral eggs does not influence dextral eggs.

在实验中，用取自右旋卵子的细胞质进行的注射，可改变左旋卵子的模式；用取自左旋卵子的细胞质进行的注射却不能影响到右旋卵子。

In experiments, an injection of cytoplasm from dextral eggs change s the pattern of sinistral eggs, but an injection from sinistral eggs does not influence dextral eggs.

在实验中，用取自右旋卵子的细胞质进行的注射，可改变左旋卵子的模式；用取自左旋卵子的细胞质进行的注射却不能影响到右旋卵子。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。