细胞计数

RESULTS: 1 Through counting method,growth curve of HepG2 was set out.

结果： 1通过细胞计数法绘出HepG2细胞的生长曲线。

With cells 7404 as the target cells, the killer role of rIL-2 and cytotoxin of LAK cells in the case of pH 6.8, 7.3 and 7.6 will be assayed one by one by the MTT method.

应用细胞计数方法观察pH值分别为6.8、7.3、7.6的培养液，对rIL-2激活单个核细胞的影响。

With cells 7404 as the target cells, the killer role of rIL-2 and cytotoxin of LAK cells in the case of pH 6.8, 7.3 and 7.6 will be a ayed one by one by the MTT method.

应用细胞计数方法观察pH值分别为6.8、7.3、7.6的培养液，对rIL-2激活单个核细胞的影响。

The normal cell growth curve of the transduced lymphocytes indicated that these cells could survival for long periods and be strained.

细胞计数测定细胞生长曲线，结果表明转染的淋巴细胞能长期存活和建株，其本身生长不受影响。

More thicker epiphyseal plate were found in the left femurs than the right femurs of the group fixed with plates at 3 and 6 month after operation. In the group fixed with plates, Safranine O staining showed epiphyseal plates ot the left femurs had more fuscous staining than the right femurs at 3 and 6 month after operation. Electron microscopy also found that the cells of the epiphyseal plates of left femurs were more eugenic than the right femurs in the group fixed with plates at 3 and 6 month after operation.

光镜学实验结果示滑动钢板固定的左侧股骨骨骺板总厚度及肥大细胞计数均明显大于普通组；组织化学观察示滑动钢板固定的左侧股骨骺板于术后3个月及6个月Safranine O组织化学染色明显深于普通组；电镜观察示滑动钢板固定的左侧股骨骨骺于术后3个月及6个月软骨增殖区软骨细胞分裂及生长较普通钢板固定的右侧活跃。

Methods Cell number counting, MTT reduction assay, transmission electron microscope were performed in skin fibroblasts,and sodium arsenite was used as tester for inorganic arsenics.

方法通过直接细胞计数法和噻唑蓝还原法检测亚砷酸钠对皮肤成纤维细胞生长的影响。

Methods Immunohistochemistry was used to detect the expression of OPN and CD44 in the LECs of anterior subcapsular cataracts(22 cases)、nuclear cataracts(17 cases)、cortical cataracts(14 cases) and normal lenses(11 cases),and positive cells were counted.

方法采用免疫组织化学法检测22例前囊下白内障1、7例核性白内障、14例皮质性白内障患者和11例正常晶状体上皮细胞中OPN和CD44的表达，并进行阳性细胞计数和各组间比较。

Proliferation of cells was analyzed by mitochondrial reduction of tetrazolium salt MTT to formazan and actual cell count.

用四甲基偶氮唑盐法和细胞计数法分析细胞增殖情况。

Medicinal vesiculation or moxibustion of wheat grain form on "Dazhui","Shenshu" and "Zusanli" has significant effect on bone marrow inh...

天灸或麦粒灸"大椎"、"肾俞"、"足三里"穴，不但可以不同程度地减轻骨髓抑制，促进骨髓增生活跃，提高骨髓有核细胞计数，提前回升外周血WBC数，缩短化疗所致WBC的持续低值期，其中尤以天灸Ⅱ、Ⅳ组的效果为佳，而且还可增强化疗小鼠巨噬细胞诱生GM－CSF的能力。

The method of vitrification cryopreservation of mouse 2-cell embryos was studied. Several vitrification solutions named EFS30, EFS40, EDFS30 and EDFS40 were prepared. The solution contains ethylene glycol, Ficoll, sucrose and DMSO. Straw method was used to vitrify and cryopreservate in vivo mouse 2-cell embryos at room temperature (20℃ or 25℃). OPS method was used to vitrify the embryo at 25 ℃ or 37℃.

本试验以毒性低、渗透性较强的乙二醇和DMSO为主体，添加聚蔗糖、蔗糖配制的玻璃化溶液(EFS30、EFS40、EDFS30和EDFS40)，在室温(20℃和25℃)下利用细管法，以及在25℃和37℃恒温台条件下OPS法对小鼠2-细胞胚胎进行玻璃化冷冻保存，并对解冻后胚胎的体外培养、囊胚细胞计数及胚胎移植技术进行了研究。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。