细胞计数

The level of tumor necrosis factore alpha, interleukin-6 (IL-6) and interleukin-8 (IL-8) were evaluated using ELISA method. Western blotting was used to detect the protein expression of protein kinase B and lipid phosphatase PTEN. Immunoprecipitations and radioactivity of γ〓P incorporated into its specific substrate histon H〓B was utilized to determine the PKB activation. Phosphate concentration released from PTEN-specific substrat diC16PIP3 was assessed using green reagent method.

以细胞计数、噻唑蓝比色法测定细胞增殖能力，酶联免疫法检测培养上清液中细胞因子TNFα、IL-6、IL-8水平，Western免疫印记定量蛋白激酶B、蛋白磷酸酶PTEN表达水平，免疫沉淀、特异底物组蛋白H2Bγ〓P掺入量测定PKB活性，特异底物dic16PIP3脱磷酸绿色试剂法反应PTEN活性。

Abnormalities initially detected on CBC included heterophilic leukocytosis and anemia; lymphocytosis and monocytosis were detected later.

全血细胞计数异常包括嗜异染的白细胞增多和贫血，淋巴细胞和单核细胞计数稍后增高。

After therapy, we compared the change of the patients' platelet count and megakaryocyte count and the time when the bleeding symptom is disappeared.

观察治疗前及治疗开始后第3、6、9、14天血小板计数，和巨核细胞计数，并统计治疗开始后出血症状消失的时间。

Objective: To explore the relationship between thrombopoietin levels and blood platelet count, megakaryocyte count in patients with thrombocytopenia and provide theoretical basis of clinical application of rhTPO.Methods: We measured blood platelet count, megakaryocyte count and the serum level of TPO in three group patients of differential diagnosis of thrombocytopenia .

目的 通过对不同类型的血小板减少患者进行血小板计数、巨核细胞计数，血小板生成素的测定，来探讨三者之间的关系及各种疾病造成血小板减少的原因，针对不同病因提出不同的治疗方案，为重组人血小板生成素的临床应用提供理论依据。

The splitting of the cytoplasm is also restrained therefore they form many multi-core cells. With the reducing of the microfilament the pseudopodium of the cell surface become fewer, shorter and wider.

结果发现，在CB作用下，癌细胞生长受到抑制，细胞计数减少；胞质分裂受到抑制，形成多核细胞；微丝结构减少，细胞表面的伪足变少变短变粗。

Bronchoalveolar lavage fluid was assessed for total and differential cell counts, and cytokine and chemokine levels.

收集支气管肺泡灌洗液，评价总细胞和不同细胞计数、细胞因子和趋化因子水平。

METHODS: LAK cell proliferation was assayed in the presence of either Tre or Ret by cell counting.

分别用细胞计数和MTT法测定LAK细胞的增殖和细胞毒作用。

Methods:SD rat dermal cells were cultivated in vitro adherently on regenerated silk fibroinfilms and observed the effects of regenerated silk fibroin films on growth curve and chromosome of cells by viable cell counts and normal detection of chromosome.

采用静置贴壁培养法在再生丝素膜上培养大鼠真皮细胞，并用活细胞计数法和常规染色体检测技术，观察再生丝素膜对细胞生长和染色体的影响。

Rats in LPS group (n = 12) were intratrachealy administered 200 μg of LPS dissolved in 100 μl saline after anesthetization, rats in control group(n = 12 ) were given intratracheal 100 μl saline.

通过检测LPS组大鼠气管注入LPS后第2、4、7天肺组织炎症指标包括肺炎症积分、支气管肺泡灌洗液中性粒细胞计数、巨噬细胞计数、TNF-α、IL-1β及IL-8含量和气道黏液分泌指标包括肺组织阿先蓝—过碘酸雪夫阳染面积、免疫组化黏蛋白Muc5ac及逆转录-聚合酶链反应Muc5ac mRNA的表达，对LPS诱发大鼠肺炎症和气道黏液高分泌的改变进行动态观察。

The methods used for collecting samples and establishing specific HAB cultures are described in detail. Methods of SEM and ESEM (environmental scanning electron microscope) for Gymnodinium were developed an...

建立了赤潮监测中样品采集处理、分离和纯化培养的技术体系；建立和优化了裸甲藻扫描电镜观察技术和环境样品的扫描电镜观察方法；提出了库尔特计数是相对简便、快捷、准确、可行的细胞计数方法；应用基于反相高效液相色谱的光合色素分析技术及原位荧光激发光谱测定方法，得出不同类群浮游植物对不同的营养盐限制存在不同响应机制的结论；用荧光强度来监测浮游植物生理状态及营养状态变化具有一定的可行性，HPLC和原位荧光两种方法指示浮游植物的生理状态存在一定差异。2。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。