细胞计数

Methods the rabbit arterial smooth muscle cells were cultured ex vivo, cells of 4-7 generation were divided into groups interfered with ang-（1-7）, positive control groupsinterfered with ang-ii(10-6mol/l) and blank control group .cell counting with trypanblau dying and mtt were used to evaluate the growth of cells in different groups and results were compared .

体外培养兔肺动脉平滑肌细胞，鉴定后传代培养，取4～7代细胞，应用不同浓度ang-（1-7）干预，并与血管紧张素-ii(ang-ii，浓度10-6mol/l)、空白组对照。通过台盼蓝染色法细胞计数、四甲基偶氮唑蓝比色法，确定肺动脉平滑肌细胞的生长趋势变化。

METHOD: The extract of sago cycas leaves was added to K562 and HL-60 cell culture fluid respectively, Cell clusters, live-cells,dead cells were calculated and cell protein was detected. The results were compared with P. B. S. control group.

细胞培养液中分别加入铁树叶(实验组1和实验组2)及P.B.S(对照组1和对照组2)，观察集落细胞数、死活细胞计数及细胞蛋白质含量。

The method of cell count was used to draw cell growth curve.

细胞计数及MTT绘制细胞生长曲线及检测细胞增殖率的变化，并同时采用TUNEL法检测细胞凋亡形态学的变化。

Methods: morphological change observation, live cell accounting and clone formation were used to investigate the inhibitory effects of Cirsium setosum MB on human chronic myloid leukemia K562 cell, hepatoma HepG2cell, cervical carcinoma Hela cell and gastric carcinoma BGC823 cell.

细胞形态观察、活细胞计数法和克隆形成率法研究小蓟水提液对人白血病细胞K562、肝癌细胞HepG2、宫颈癌细胞Hela、胃癌细胞BGC823生长的抑制作用。

By FCM analy sing, p53 gene can induce cells apoptosis and make it G1 arrest; Cisplatine can induce cell apoptosis and make it G2 arrest.

流式细胞计数证实p53能诱导QBC939细胞发生凋亡并导致其发生G1期阻滞，顺铂能诱导QBC939细胞发生凋亡并导致细胞发生明显的G2期阻滞。

The biological features of hBMSCs and cryogentic storaged cells were analyzed.hBMSCs memberane antigens were identified by immunofluorescence staining.The potential osteogenous differentiation of hBMSCs was evaluated by the osteogeneic inducer kit.

倒置光学显微镜下观察细胞形态及生长情况，细胞计数法绘制细胞生长曲线，并分析冻存细胞复苏后的生长特性，免疫荧光染色测定hBMSCs表面标志，成骨诱导试剂盒检测hBMSCs向成骨分化的潜能。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜，用茜素红-台盼蓝染色染色行角膜内皮细胞计数；用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变；并于正常对照组角膜比较。

On human carcinoma cells.MethodsMorphological change observation and live cell accounting were used to investigate the inhibitory effects of Sanguisorba officinalis L.

方法细胞形态观察和活细胞计数法研究地榆水提液对人白血病细胞K562，肝癌细胞HepG2，胃癌细胞BGC823，宫颈癌细胞Hela生长的抑制作用。

METHODS: SGC7901 cells were transfected with siRNAs targeted against the human PLK1 by chemosynthesis. The PLK1 mRNA levels of cells transfected with siRNAs were monitored by real time PCR and the protein levels were detected by immunocytochemistry. Cell proliferation was evaluated by direct cell counting with trypan blue staining.

化学合成针对PLK1的小干扰RNA，转染至胃癌细胞株SGC7901中，利用实时定量PCR、免疫细胞化学、台盼蓝活性细胞计数和流式细胞术对PLK1的基因表达、细胞增殖、细胞周期和凋亡的检测。

In vivo treated sera from ATG-treated patients produced positive test results for T CDC and T/B FXM, while the B-cell CDC crossmatch remained negative.

来自于ATG治疗的病人血清测试发现，引起T细胞的补体依赖细胞毒性反应和 T/B的流式细胞计数阳性结果相关，而同时，B细胞补体依赖细胞毒性交叉配血保持阴性。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。