细胞计数

Methods after immunohistochemical staining,light microscope was employed to determine expressions of vegf and tams. statistical analysis was performed by using spss software.

采用免疫宫颈腺癌em化学染色，在光镜下对vegf阳性细胞评分及tams计数进行分析。

Methods Immunohistochemical staining was carried out to measure microvessel counts and interstitial infiltrating TAMs, MCs counts in 40 NSCLC tissues.

用免疫组化ABC法测定40例非小细胞肺癌组织石蜡切片标本中MV和TAMs、MCs计数。

Methods After immunohistochemical staining, light microscope was employed to determine expressions of VEGF and TAMs. Statistical analysis was performed by using SPSS software.

目的 研究胰腺癌组织中血管内皮生长因子(vascular endothelial growth factor，VEGF)表达和肿瘤相关巨噬细胞(tumourassociated macrophages，TAMs)计数与淋巴结转移的关系。

Results In 40 NSCLC tissues, Between MV, TAMs, MCs counts and IL-8, MCP-1. MIP-1 mRNA positive expressions shown mutually positive correlation.

结果 40例非小细胞肺癌组织中MV、TAMs、MCs计数和IL-8、MCP-1、MIP-1阳性表达之间以及它们相互之间均存在着密切的正相关。

Methods To detect lymphocyte subset in whole blood with different preservers by flow cytometric multi-color analysis.

流式细胞术多色分析方法计数不同保存剂处理后、不同时间内血液中淋巴细胞亚群。

All the indexes were tested in 2 -. 4 .. 6 weeks of operative intervals respectively. Results:(1) The general observation in the experimental group ,such as spirit, hair, body weight and activity ,was superior to that in the control group.(2) The recovery rate of sciatic nerve functional index (SF1) was statistically significantly higher in the experimental group than in the control group .(3) electrophysciological indexes: m the experimental group ,the tardy rate of induced motorius action potential ,recovery rates of the amplitude of compound muscle action potential and twitch tension and tetanic tension of triceps surae muscle ,were significantly higher than in the control group.(4) Compared with the control group ,the triceps surae muscle wet weight was significantly greater in the experimental group.(5) histomorphological indexes: the count of myelinated nerve fibers ,diameter of regenerated axon, thickness of nerve myelin sheath and area of capillary were much more in the experimental group than in the control group.(6) observation of ultrastructure with the transmission election microscope: more abundant organell a and maturer myelin sheath lay in the injured peripheral nerve of the experimental group, while not lay in the control group.

分别在木后第2、4、6周时进行各项指标的检测，结果发现：（1）实验组SD大鼠在整个实验过程中，其精神、毛发、体重，运动灵活性等一般情况优于对照组；（2）实验组的坐骨神经功能指数恢复率显著优于对照组，P＜0.01；（3）神经电生理指标：实验组运动神经诱发电位潜伏期的延迟率显著优于对照组，P＜0.05；复合肌肉动作电位振幅的恢复率显著高于对照组，两组比较第2周时P＜0.01，第4、6周时，P＜0.05；小腿三头肌单收缩力和强直收缩力的恢复率实验组显著高于对照组，P＜0.01；（4）小腿三头肌湿重的恢复率，第2、4周时实验组高于对照组，差异非常显著，P＜0.01；第6周时，差异仍显著，P＜0.05；（5）组织形态学指标：有髓种经轴突计数、再生轴突直径的恢复率，毛细血管面积，实验组均显著大于对照组，P＜0.01、P＜0.05、P＜0.001；实验组髓鞘厚度的恢复率也显著高于对照组，第2周时P＜0.01，第4、6周时，P＜0.05；（6）透射电镜超微结构观察：实验组再生轴突中细胞器丰富，髓鞘结构成熟，神经再生情况优于对照纽。

Firstly, we analyze the color feature of leukocyte images, and then apply an algorithm of segmentation for nucleus extraction based on the HSI color space, which establishes a foundation for the detection of leukocytes. For the localization and the counting of leukocytes, we use an efficient algorithm for labeling connected region to obtain several separate single-cell windows.

首先，我们讨论并分析了白细胞图像的彩色特征，提出了在HSI彩色空间中利用饱和度分割得到细胞核的方法，从而为白细胞的检出奠定了基础，在白细胞的计数和定位方面，主要采用了简捷的连通域标记算法，检出若干包含单个白细胞的细胞窗。

After 72 hours 3H-TdR were incorporated into culture matrix fluid and double channel liquid scintillation counter was used for measurement of radiactivity count per minute.

测定2，6，24 h细胞贴壁率，培养72 h进行3H-TdR掺入试验，双道液体闪烁计数器测定各组的放射性计数。

Statistical data of VEGF grading scores showed that its expression was not significantly related to the size and location of glioma, sex, and age of patients, but closely related to the glioma gradings. The expression of TF was positively correlated with that of VEGF, suggesting that TF might participate in the anigogenesis of gliomas, and provide proper environment for the growth of gliomas through its coagulant effect.

根据VEGF分级计数统计学分析，显示VEGF表达与肿瘤的大小、部位及患者性别、发病年龄等临床因素无显著相关性，而与肿瘤的组织学分级密切相关。3、胶质细胞瘤中TF与VEGF的表达正相关，TF可能参与了肿瘤血管的生成过程，并通过其促凝血活性作用为胶质瘤的生长创造适宜的内环境。

Statistical data of TF grading scores showed that its expression was not significantly related to the size and location of glioma, sex, and age of patients, but closely related to the glioma gradings and microvessel quantity of the tumor.VEGF was found in gliomas of all gradings and its expression was significantly enhanced with the increase of glioma gradings.

TF分级计数统计结果显示其表达与肿瘤的大小、部位及患者性别、发病年龄等临床因素无显著相关性，但与肿瘤的分级及肿瘤微血管数量密切相关。2、VEGF在各级胶质细胞瘤组织中均有表达，随着肿瘤组织学分级的增加，VEGF的表达明显增强。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。