细胞计数

Methods Fifty F-344 rats were divided into four groups, GM only、PAA+GM、PAA only and saline control. ABR thresholds at different frequencies were measured at different times and hair cell losses were numerated. Two-dimensional diffusion assays in the culture medium were performed to evaluate the effect of PAA on the antimicrobial activity.

选用健康F-344大鼠50只，随机分4组：Ⅰ为GM、Ⅱ为PAA+GM、Ⅲ为PAA、Ⅳ为生理盐水对照组；通过观测4组大鼠不同时期、不同频率听性脑干反应（auditory brainstem respons，ABR）阈值的改变；计数耳蜗毛细胞死亡率，以观察PAA对F-344大鼠GM耳蜗毒性的拮抗作用；用双向扩散血清培养基检测法观察PAA 对 GM 抗菌活性的影响。

All mice were killed by cutting neck on 7th day,ulnas and femur were taken out.The bone marrow histologic morphology and ultrastructure of mice ulnas were ovserved.

于第7天断颈处死小鼠，取出尺骨和股骨，计数骨髓单个核细胞，并行尺骨组织学切片和超微结构观察。

Experiment 1, let rats acquire taste preference to quinine solution, and take account of β-EP-immunoreactive neurons in different nucleuses of hypothalamic areas, separately adopted taste preference learning training and immunohistochemistry; Experiment 2, Added banana essence into quinine solution to discuss the connection between flavor preference learning and the expression of β-EP; Experiment 3, To research endogenous opiate like substances contribute to the establishment of CTP and influence the behavior of taste preference by naloxone was i.p.

实验一，采用味觉嗜好学习训练和免疫组织化学方法，让大鼠获得对奎宁的味觉嗜好，计数了下丘脑不同核团内β-内啡肽免疫反应细胞数；实验二，向奎宁溶液中加入香蕉气味，探讨风味嗜好学习与下丘脑β-内啡肽表达的关系；实验三，腹腔内注射纳络酮，探讨内阿片肽对CTP建立及味觉嗜好行为表达的影响。

Objective:Discusses the BC-3000plus entire automatic blood corpuscle analyzer white blood cell classification to count with the manual classified result difference,understands the instrument dialogue cell classification the accuracy.

手工法与BC－3000血球分析仪白细胞分类的比较目的：探讨BC-3000plus全自动血细胞分析仪白细胞分类计数与手工分类结果的差异，以了解仪器对白细胞分类的准确性。

In order to discuss the harm of long-time surfing the internet to the human body, we extract 35 undergraduate in our university and carry on the net continuously experiment. At the same time, 23 people (male 15 person, female 8 person) have been sampled on the track inspection on routine blood index. We discovered that continual 56 hour surfers can c ause obvious blood corpuscle metabolism function disorder, There are radical ascensions concerning the absolute value of blood routine inter cells, the number of white blood cells, the volume and quantity of the blood platelet and so on. The average volume of the red blood cells reduces significantly. The distributing volume and width of the red blood cells increase correspondingly.

为了探讨长时间上网对人体的危害，我们抽取在校本科大学生35人进行连续上网实验，对其中的23人(男15人、女8人)进行了跟踪血常规检查，结果发现连续56小时的上网，导致受试者出现明显的血细胞代谢功能紊乱，血常规中间细胞绝对值、白细胞计数、血小板体积及数量等多项指标出现应激性升高，平均红细胞体积显著性降低，红细胞体积分布宽度百分比明显增高。

The results of laboratory studies performed on admission revealed an elevated white-cell count of 18,200 per cubic millimeter, with 76 percent neutrophils, 9 percent lymphocytes, 11 percent monocytes, 3 percent eosinophils, and 1 percent basophils.

患者入院时实验室检查结果示白细胞计数增高，18，200mm3，中性粒细胞76%，淋巴细胞9%，单核细胞11%，嗜酸性粒细胞3%，嗜碱性粒细胞1%。

After calculating the concentration by counting board, cell suspension was cultured on the coverslips coated poly-L-lysine at 1×109/L, then the coverslips were paved in the bottom of 35 mm culture dish for 3 weeks.

经12 h+ 24 h两次差速培养后，吸出细胞上悬液，计数板计算浓度后，按1×109 L-1浓度重新种植于载有多聚赖氨酸包被盖玻片的35 mm培养皿中继续培养3周。

MAIN OUTCOME MEASURES: One week later, percentage of rat peripheral blood leucocytes and mononuclear cells was counted in each group. Heart function was compared among groups 4 weeks later.

主要观察指标：1周后计数3组大鼠外周血白细胞及单个核细胞百分比，4周后比较3组大鼠在体心功能。

Methods After immunohistochemical staining, light microscope was employed to determine expressions of VEGF and TAMs. Statistical analysis was performed by using SPSS software.

采用免疫组织化学染色，在光镜下对VEGF阳性细胞评分及TAMs计数进行分析。

Methods after immunohistochemical staining,light microscope was employed to determine expressions of vegf and tams. statistical analysis was performed by using spss software.

采用t型淋巴癌是什么组织化学染色，在光镜下对vegf阳性细胞评分及tams计数进行分析。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。