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细胞计数

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Methods Twelve healthy volunteers, of them 5 smokers and another 7 nonsmokers, underwent right middle lobe bronchoalveolar lavage and transbronchial instillation of KLH into lingula. Dimeric and monomeric IgA, albumin and cellular differential count in BALF were measured. Serum anti-KLH IgA was tested at various days after instillation of antigen.

对12名健康志愿者(其中5名吸烟)行右肺中叶肺灌洗,分析肺灌洗中IgA型别、细胞分类计数、白蛋白含量,并从左肺舌肺叶内注入钥孔血蓝蛋白抗原,观察肺局部抗原免疫后血循环中特异性IgA抗体的免疫应答能力。

The CBC here shows a markedly increased MCV, typical for megaloblastic anemia.

全血细胞计数显示MCV明显地升高,是典型的巨幼细胞贫血的表现。

Results Erythrocyte count, hemoglobin concentration and hematocrit of all the patients with malignant digestive tumor decreased, mean corpuscular volume and mean corpuscular hemoglobin increased, arid there was inconspicuous change in total leukocyte count. The indications of megaloblastic anemia and proliferative anemia in bone marrow hemogram were found in the patients. It was common that the percentage of neutrophils was higher and that of lymphocytes was lower than that of the normal controls.

结果 消化道恶性肿瘤患者的红细胞计数、血红蛋白、红细胞压积均减少,平均红细胞体积、平均红细胞血红蛋白量增高,均表现为巨幼细胞性贫血,骨髓象呈增生性贫血改变,而白细胞总数的变化较小,白细胞分类,中性粒细胞百分比超过正常范围与淋巴细胞百分比低于正常范围者多见,血小板数异常远较血红蛋白与白细胞为少,但36例肝癌患者中28例出现血小板减少。

By chromosome counting, the detected hybrids were mixoploid.

通过染色体计数,发现所检测杂种为混倍体,同一植株的细胞有多种染色体数目构成。

ICH was induced by the injection of 50 ul autologous blood into the right caudate nucleus, while 50 μl natural saline was injected in the control group. The rats were killed after 6h, 24h, 2d, 3d, 5d and 7d respectively, the morphology changes of nerve cell oncosis and count by electron microscope and the immunohistochemical method and computer image analysis were performed in rats brain by using antibody of VEGF.

采用二步法制备大鼠脑出血模型,脑出血组注入自体血50μl,对照组注入等量生理盐水,分别于6、24h、2、3、5、7d后处死,行HE染色和免疫组化染色观察脑内VEGF表达变化;电镜下观察胀亡细胞形态学变化并计数。

Electrophysciological indexes: in the experimental group ,the tardy rate of induced motorius action potential ,recovery rates of the amplitude of compound muscle action potential and twitch tension and tetanic tension of triceps surae muscle ,were significantly higher than in the control group.(4) Compared with the control group ,the triceps surae muscle wet weight was significantly greater in the experimental group.(5) histomorphological indexes: the count of myelinated nerve fibers ,diameter of regenerated axon,thickness of nerve myelin sheath and area of capillary were much more in the experimental group than in the control group.(6) observation of ultrastructure with the transmission election microscope: more abundant organella and maturer myelin sheath lay in the injured peripheral nerve of the experimental group, while not lay in the control group.

分别在术后第2、4、6周时进行各项指标的检测,结果发现:(1)实验组SD大鼠在整个实验过程中,其精神、毛发、体重,运动灵活性等一般情况优于对照组;(2)实验组的坐骨神经功能指数恢复率显著优于对照组,P<0.01;(3)神经电生理指标:实验组运动神经诱发电位潜伏期的延迟率显著优于对照组,P<0.05;复合肌肉动作电位振幅的恢复率显著高于对照组,两组比较第2周时P<0.01,第4、6周时,P<0.05;小腿三头肌单收缩力和强直收缩力的恢复率实验组显著高于对照组,P<0.01;(4)小腿三头肌湿重的恢复率,第2、4周时实验组高于对照组,差异非常显著,P<0.01;第6周时,差异仍显著,P<0.05;(5)组织形态学指标:有髓神经轴突计数、再生轴突直径的恢复率,毛细血管面积,实验组均显著大于对照组,P<0.01、P<0.05、P<0.001;实验组髓鞘厚度的恢复率也显著高于对照组,第2周时P<0.01,第4、6周时,P<0.05;(6)透射电镜超微结构观察:实验组再生轴突中细胞器丰富,髓鞘结构成熟,神经再生情况优于对照组。

Cells in the bron choalveolar lavage fluid were counted and differentiated, as well as the amounts of total protein, albumin and the activities of alkaline phos phatase, lactate dehydrogenase and type Ⅳ collagenase (matrix metallco proteinases, MMP-2, MMP-9) were detected.

计数支气管肺泡灌洗液中的细胞数并行分类,测定总蛋白、白蛋白含量及碱性磷酸酶、乳酸脱氢酶及Ⅳ型胶原酶(MMP-2、MMP-9)活性。

Lymphocyte stimulation index was calculated after counting the flares using β liquid scintilloscope.

同时建立成骨细胞、淋巴细胞混合培养模型,利用β液闪计数仪测定每分钟闪烁数,计算淋巴细胞刺激指数。

Methods TGFβ1 protein in 53 cases of RA synovia [including 26 cases of RA,20 cases of control non-RA (14 cases of OA and 6 cases of traumatic arthritis) and 7 cases of normal synovia] was detected by immunohistochemical LSAB method.

采用免疫组织化学LSAB法检测了53例(其中26例RA,20例其他关节炎和7例正常人)滑膜组织中TGFβ1蛋白的表达情况,并用目镜网格测微尺和图像分析系统分别进行TGFβ1阳性细胞的半定量计数和TGFβ1染色强度及面积的定量分析。

The quantity and appearance of Foslike immunopositive neurons in ventrolateral part of PAG were investigated by immunocytochemical staining method.

采用免疫组织化学ABC法对大鼠中脑冰冻切片进行染色,显微镜下进行观察并拍照,对Fos免疫阳性细胞进行形态与计数分析。

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